Effects of Acetoin Addition on 2,3-butanediol Production by Saccharomyces cerevisiae W5/W141

doi:10.11924/j.issn.1000-6850.casb20190500192

Abstract

Abstract:

To obtain a 2,3-butanediol (2,3-BD) producing strain with good performance, different concentrations of acetoin were added into two strains of Saccharomyces cerevisiae (W5/W141) producing 2,3-BD, and the changes of 2,3-BD, ethanol and glycerol production with fermentation time were determined. The optimum acetoin concentration of S. cerevisiae W5 was 12 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, it was 2.54±0.03 g/L, and the conversion of glycerol and ethanol decreased by 17.6% and 23.6% respectively compared with that without addition. The optimum acetoin concentration of S. cerevisiae W 141 was 10 g/L, and the concentration of 2,3-BD reached the maximum at 72 h, it was 1.71±0.02 g/L, and the conversion of glycerol and ethanol decreased by 57.1% and 16.7% respectively. By comparison, S. cerevisiae W5 was selected as the optimum strain to provide new resources for the production of 2,3-BD by microbial fermentation.

Key words: 2,3-butanediol, Saccharomyces cerevisiae, acetoin, ethanol, glycerol

CLC Number:

Q935

Yang Zhiyu, Tong Tianqi, Liu Lei, Ping Wenxiang, Ge Jingping. Effects of Acetoin Addition on 2,3-butanediol Production by Saccharomyces cerevisiae W5/W141[J]. Chinese Agricultural Science Bulletin, 2020, 36(23): 19-25.

Figures/Tables 10

References 22

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产物浓度	菌株		比值
产物浓度	S. cerevisiae W5	S. cerevisiae W5-12 g/L	比值
甘油/(g/L)	3.00±0.04 a(60 h)	2.73±0.03 a(48 h)	下降26.7%
乙醇/(g/L)	54.78±0.07 b(60 h)	44.05±1.09 a(36 h)	下降24.4%
2,3-BD/(g/L)	—	2.54±0.02(72 h)	—
甘油转化率/(g/g)	0.04±0.02 a(60 h)	0.034±0.002 b(48 h)	下降17.6%
乙醇转化率/(g/g)	0.68±0.03 b(60 h)	0.55±0.01 a(36 h)	下降23.6%
2,3-BD转化率/(g/g)	—	0.032±0.001(72 h)	—
甘油生产强度/[g/[L·h)]	0.20±0.03 a(12 h)	0.11±0.02 b(12 h)	下降81.8%
乙醇生产强度/[g/[L·h)]	2.67±0.06 b(12 h)	3.27±0.03 a(12 h)	提高22.5%
2,3-BD生产强度/[g/[L·h)]	—	0.035±0.001(72 h)	—
单个细胞甘油生产强度	0.40±0.03 a(24 h)	0.60±0.03 b(24 h)	提高50.0%
单个细胞乙醇生产强度	5.23±0.07 b(48 h)	5.34±0.09 a(24 h)	提高2.13%
单个细胞2,3-BD生产强度	—	0.356±0.002(72 h)	—

产物浓度	菌株		比值
产物浓度	S. cerevisiae W5	S. cerevisiae W5-12 g/L	比值
甘油/(g/L)	3.00±0.04 a(60 h)	2.73±0.03 a(48 h)	下降26.7%
乙醇/(g/L)	54.78±0.07 b(60 h)	44.05±1.09 a(36 h)	下降24.4%
2,3-BD/(g/L)	—	2.54±0.02(72 h)	—
甘油转化率/(g/g)	0.04±0.02 a(60 h)	0.034±0.002 b(48 h)	下降17.6%
乙醇转化率/(g/g)	0.68±0.03 b(60 h)	0.55±0.01 a(36 h)	下降23.6%
2,3-BD转化率/(g/g)	—	0.032±0.001(72 h)	—
甘油生产强度/[g/[L·h)]	0.20±0.03 a(12 h)	0.11±0.02 b(12 h)	下降81.8%
乙醇生产强度/[g/[L·h)]	2.67±0.06 b(12 h)	3.27±0.03 a(12 h)	提高22.5%
2,3-BD生产强度/[g/[L·h)]	—	0.035±0.001(72 h)	—
单个细胞甘油生产强度	0.40±0.03 a(24 h)	0.60±0.03 b(24 h)	提高50.0%
单个细胞乙醇生产强度	5.23±0.07 b(48 h)	5.34±0.09 a(24 h)	提高2.13%
单个细胞2,3-BD生产强度	—	0.356±0.002(72 h)	—

产物浓度	菌株		比值
产物浓度	S. cerevisiae W141	S. cerevisiae W141-10 g/L	比值
甘油/(g/L)	3.36±0.04 a(60 h)	2.09±0.04 b(26 h)	下降59.0%
乙醇/(g/L)	54.90±0.98 a(60 h)	45.13±0.11 b(60 h)	下降15.6%
2,3-BD/(g/L)	—	1.71±0.04(72 h)	—
甘油转化率/(g/g)	0.04±0.01 a(60 h)	0.03±0.01 b(36 h)	下降57.1%
乙醇转化率/(g/g)	0.69±0.03 a(60 h)	0.56±0.02 b(60 h)	下降16.7%
2,3-BD转化率/(g/g)	—	0.02±0.001(72 h)	—
甘油生产强度/[g/[L·h)]	0.12±0.01 a(24 h)	0.12±0.01 b(12 h)	下降58.3%
乙醇生产强度/[g/[L·h)]	2.92±0.08 a(12 h)	2.58±0.04 b(12 h)	下降15.7%
2,3-BD生产强度/[g/[L·h)]	—	0.024±0.001(72 h)	—
单个细胞甘油生产强度	0.67±0.01 a(48 h)	0.72±0.02 b(12 h)	提高7.4%
单个细胞乙醇生产强度	7.93±0.12 a(24 h)	7.98±0.09 b(24 h)	提高0.6%
单个细胞2,3-BD生产强度	—	0.297±0.004(72 h)	—

产物浓度	菌株		比值
产物浓度	S. cerevisiae W141	S. cerevisiae W141-10 g/L	比值
甘油/(g/L)	3.36±0.04 a(60 h)	2.09±0.04 b(26 h)	下降59.0%
乙醇/(g/L)	54.90±0.98 a(60 h)	45.13±0.11 b(60 h)	下降15.6%
2,3-BD/(g/L)	—	1.71±0.04(72 h)	—
甘油转化率/(g/g)	0.04±0.01 a(60 h)	0.03±0.01 b(36 h)	下降57.1%
乙醇转化率/(g/g)	0.69±0.03 a(60 h)	0.56±0.02 b(60 h)	下降16.7%
2,3-BD转化率/(g/g)	—	0.02±0.001(72 h)	—
甘油生产强度/[g/[L·h)]	0.12±0.01 a(24 h)	0.12±0.01 b(12 h)	下降58.3%
乙醇生产强度/[g/[L·h)]	2.92±0.08 a(12 h)	2.58±0.04 b(12 h)	下降15.7%
2,3-BD生产强度/[g/[L·h)]	—	0.024±0.001(72 h)	—
单个细胞甘油生产强度	0.67±0.01 a(48 h)	0.72±0.02 b(12 h)	提高7.4%
单个细胞乙醇生产强度	7.93±0.12 a(24 h)	7.98±0.09 b(24 h)	提高0.6%
单个细胞2,3-BD生产强度	—	0.297±0.004(72 h)	—

产物浓度	菌株		比值
产物浓度	S. cerevisiae W5-12 g/L	S. cerevisiae W141-10 g/L	比值
甘油/(g/L)	2.73±0.03 a(48 h)	2.09±0.04 b(26 h)	下降30.6%
乙醇/(g/L)	44.05±1.09 a(36 h)	45.13±0.11 b(60 h)	提高2.5%
2,3-BD/(g/L)	2.54±0.02(72 h)	1.71±0.04(72 h)	下降48.5%
甘油转化率/(g/g)	0.034±0.002 b(48 h)	0.03±0.01 b(36 h)	下降13.3%
乙醇转化率/(g/g)	0.55±0.01 a(36 h)	0.56±0.02 b(60 h)	提高18.1%
2,3-BD转化率/(g/g)	0.032±0.001(72 h)	0.02±0.001(72 h)	下降60.0%
甘油生产强度/(g/L·h)	0.11±0.02 b(12 h)	0.12±0.01 b(12 h)	提高9.1%
乙醇生产强度/(g/L·h)	3.27±0.03 a(12 h)	2.58±0.04 b(12 h)	下降26.7%
2,3-BD生产强度/(g/L·h)	0.035±0.001(72 h)	0.024±0.001(72 h)	下降45.8%
单个细胞甘油生产强度	0.60±0.03 b(24 h)	0.72±0.02 b(12 h)	提高20.0%
单个细胞乙醇生产强度	5.34±0.09 a(24 h)	7.98±0.09 b(24 h)	提高49.4%
单个细胞2,3-BD生产强度	0.356±0.002(72 h)	0.297±0.004(72 h)	下降19.8%