Potential Saccharomyces cerevisiae Strains of Producing 2,3-butanediol: Screening

doi:10.11924/j.issn.1000-6850.casb20190700383

Abstract

Abstract:

The aims are to obtain the potentially high-production 2,3-butanediol Saccharomyces cerevisiae, and study the 2,3-butanediol producing potential of different strains. The most promising Saccharomyces cerevisiae strain W141 for producing 2,3-butanediol was screened by morphological observation, product tolerance detection, key enzyme activity assay, acetoin yield and fermentation test. The results showed that the W141 grew rapidly, could withstand 13% (w/v) ethanol and 0.8% (w/v) acetic acid, respectively, and had the high ethanol production (0.593 g/g) and carbon source utilization rate (the glucose consumption rate was 100% after fermentation for 24 h). The enzyme activities of key enzymes α-ALS and BDH were higher, 0.046 U/mg and 0.040 U/mg, respectively. The key intermediate of the 2,3-butanediol pathway, acetoin, had a high content, which reached 0.246 g/L. The study indicates that Saccharomyces cerevisiae W141 has certain transformation space and application prospect in the fermentation industry.

Key words: 2,3-butanediol, Saccharomyces cerevisiae, strain screening, fermentation, key enzyme activity, strain metabolite

CLC Number:

S182

Ding Hao, Liu Wenjuan, Sun Jian, Liu Lei, Ping Wenxiang, Ge Jingping. Potential Saccharomyces cerevisiae Strains of Producing 2,3-butanediol: Screening[J]. Chinese Agricultural Science Bulletin, 2020, 36(24): 107-115.

Figures/Tables 9

References 25

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