Auxin Response Factor (ARF) Gene Family in Glycyrrhiza uralensis Fisch.: Identification and Expression Analysis

doi:10.11924/j.issn.1000-6850.casb2020-0304

Abstract

Abstract:

The purpose of the study is to identify the auxin response factor (ARF) gene family in Glycyrrhiza uralensis Fisch. and analyze their expression patterns under abiotic stress. G. Uralensis Fisch. was taken as the object. We identified the ARF gene family by bioinformatics tools, and analyzed the physicochemical properties of proteins, conserved motifs, gene structure, evolutionary relationships, cis-elements and expression profiles. 10 ARF genes (GuARFs) were identified. The length of these ARF proteins ranges from 301 to 945 amino acids and the relative molecular weight varies from 33.07 to 104.37 kDa, with PIs in the range of 5.93 to 8.50. Subcellular localization assays shows that the GuARF proteins are located in the nucleus. The conserved domain analysis indicates that most of GuARF proteins contain B3, Auxin_resp and Aux/IAA domain. Gene structure analysis reveals that the exon number of GuARF genes ranges from 6 to 22. There are 5 different types of cis-elements in the promoter region of GuARF genes. Phylogenetic analysis reveals that the GuARF proteins are clustered into 3 groups. RNA-seq data show that the expression level of 10 GuARF genes is different under abiotic stresses. The results indicate that the GuARF gene family may involve in multiple biological processes, and the study could provide valuable information for further research on physiological and molecular response of plants to abiotic stresses.

Key words: Glycyrrhiza Uralensis Fisch., auxin response factor, phylogeny, gene expression, expression pattern analysis

CLC Number:

S567.2

Guan Sijing, Gao Jing, Xu Rongrong, Ge Tiantian, Wang Nan, Yan Yonggang, Zhang Gang, Chen Ying, Liu Aping, Cheng Mengge. Auxin Response Factor (ARF) Gene Family in Glycyrrhiza uralensis Fisch.: Identification and Expression Analysis[J]. Chinese Agricultural Science Bulletin, 2021, 37(29): 20-27.

Figures/Tables 7

References 28

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基因名称	基因号	氨基酸数/aa	分子量/kDa	等电点	不稳定系数	亚细胞定位
GuARF1	Glyur000846s00019247.1	301	33.07	5.93	56.52	Nucleus
GuARF2	Glyur000322s00020885.1	606	66.04	6.14	40.06	Nucleus
GuARF3	Glyur000066s00006209.1	749	82.15	7.59	47.87	Nucleus
GuARF4	Glyur000081s00006796.1	711	78.74	6.64	51.32	Nucleus
GuARF5	Glyur002712s00037647.1	665	73.65	8.50	43.03	Nucleus
GuARF6	Glyur001170s00029947.1	945	104.37	6.06	64.68	Nucleus
GuARF7	Glyur000250s00014115.1	646	72.05	7.68	57.28	Nucleus
GuARF8	Glyur000413s00015469.1	856	95.42	6.27	61.46	Nucleus
GuARF9	Glyur000325s00013489.1	725	80.45	7.81	52.50	Nucleus
GuARF10	Glyur000156s00011992.1	780	87.06	6.08	52.35	Nucleus

基因名称	基因号	氨基酸数/aa	分子量/kDa	等电点	不稳定系数	亚细胞定位
GuARF1	Glyur000846s00019247.1	301	33.07	5.93	56.52	Nucleus
GuARF2	Glyur000322s00020885.1	606	66.04	6.14	40.06	Nucleus
GuARF3	Glyur000066s00006209.1	749	82.15	7.59	47.87	Nucleus
GuARF4	Glyur000081s00006796.1	711	78.74	6.64	51.32	Nucleus
GuARF5	Glyur002712s00037647.1	665	73.65	8.50	43.03	Nucleus
GuARF6	Glyur001170s00029947.1	945	104.37	6.06	64.68	Nucleus
GuARF7	Glyur000250s00014115.1	646	72.05	7.68	57.28	Nucleus
GuARF8	Glyur000413s00015469.1	856	95.42	6.27	61.46	Nucleus
GuARF9	Glyur000325s00013489.1	725	80.45	7.81	52.50	Nucleus
GuARF10	Glyur000156s00011992.1	780	87.06	6.08	52.35	Nucleus