Effects of Planting Densities on Growth and Physiological Characteristics of Achyranthes bidentata Blume in Tongliao

doi:10.11924/j.issn.1000-6850.casb2021-0086

Abstract

Abstract:

To explore the optimal cultivation density of Achyranthes bidentate Blume in Tongliao could provide certain reference for the standardized cultivation of Achyranthes bidentate Blume in this area. Using the method of combining field experiment and indoor measurement, a single-factor completely random design was carried out, and 5 different cultivation densities were set. The number of plants per hectare was about D1: 834000, D2: 556000, D3: 417000, D4: 333500, and D5: 278000. The growth and physiological indexes of Achyranthes bidentate Blume cultivated under different densities and the content of active ingredients during the harvest period were determined. The growth of Achyranthes bidentate Blume at the seedling stage of density D1 is relatively good, and the growth of the above-ground part is significantly restricted during the harvesting period, and the fresh weight of the above-ground part of single plant is significantly lower than that of D5. With the decrease of cultivation density, the chlorophyll content of Achyranthes bidentate Blume leaves gradually increased, and the relative conductivity gradually decreased. The chlorophyll content of the leaves under D4 and D5 was significantly higher than that of D1, and the relative conductivity of the leaves of Achyranthes bidentate Blume under D1 was extremely and significantly higher than that under D3, D4 and D5. There was no significant difference in the soluble sugar, soluble protein, malondialdehyde, SOD and POD of Achyranthes bidentate Blume leaves with different planting densities. There was also no significant difference in the content of β-ecdysterone in the roots during harvest. Considering the factors of yield and effective ingredient contents, the recommended planting density of Achyranthes bidentate Blume in Tongliao is about 834000 plants per hectare (D1).

Key words: Achyranthes bidentate Blume in Tongliao, planting density, yield, active ingredients, growth and physiological characteristics

CLC Number:

S567.21

BAO Jinhua, LI Xuxin, SUN Dezhi, JIA Junying, SHEN Xiangjun, YANG Hengshan. Effects of Planting Densities on Growth and Physiological Characteristics of Achyranthes bidentata Blume in Tongliao[J]. Chinese Agricultural Science Bulletin, 2021, 37(36): 66-72.

Figures/Tables 10

References 23

[1]	张永清. 药用植物栽培学[M]. 北京: 中国中医药出版社, 2013:298-303.
[2]	胡婷婷, 汪凤, 李桂荣, 等. 怀牛膝和川牛膝研究现状及未来研究方向探究[J]. 生物化工, 2020, 6(1):145-147.
[3]	王允荣, 蒋学杰. 怀牛膝标准化种植技术[J]. 特种经济动植物, 2016, 12:36-37.
[4]	于佳月. 土壤条件对怀牛膝品质的影响研究进展[J]. 南方农业, 2019, 13(13):38-40.
[5]	李询, 董诚明, 刘丹, 等. 氮磷钾肥配施对怀牛膝产量和质量的影响[J]. 黑龙江农业科学, 2020, 3:69-73.
[6]	杜真辉, 董诚明, 夏伟, 等. 不同土壤条件下怀牛膝质量评价[J]. 中国现代中药, 2016, 18(9):1164-1166.
[7]	李曼, 董诚明, 王鑫源. 植物生长调节剂对怀牛膝产量及品质的影响[J]. 现代园艺, 2020, 9:16-18.
[8]	田竞, 袁强华. 川牛膝质量控制的研究进展[J]. 化工设计通讯, 2019, 45(2):184-185.
[9]	MARIE E, JOACHIM S, KATJA T. Yield performance of Russian dandelion transplants (Taraxacum koksaghyz L. Rodin) in flat bed and ridge cultivation with different planting densities[J]. European journal of agronomy, 2018,126-134.
[10]	王晓飞, 魏国江, 关向军等. 密度与环境因子对蒙古黄芪育苗影响研究[J]. 中国农学通报, 2020, 36(15):43-47.
[11]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[12]	国家药典委员会. 中国药典[M].2015 版, 北京: 中国医药科技出版社, 2015: 3-4,118-124.
[13]	ZHANG H, HOU D P, PENG X L. Optimizing integrative cultivation management improves grain quality while increasing yield and nitrogen use efficiency in rice[J]. Journal of integrative agriculture, 2019,2716-2731.
[14]	林明, 白晓山, 刘华君, 等. 膜下滴灌甜菜最适种植密度的研究[J]. 新疆农业科学, 2016, 53(7):1245-1250.
[15]	马卉, 牛玉萍, 夏军, 等. 滴灌模式和种植密度对棉花叶片衰老特性的影响[J]. 新疆农业科学, 2017, 54(11):1972-1982. doi: 10.6048/j.issn.1001-4330.2017.11.002
[16]	王昱, 范杰英, 王玮, 等. 不同密度对大豆生理特性的影响[J]. 黑龙江省农业科学, 2012, 8:38-40.
[17]	陈菁瑛, 苏海兰, 黄颖桢, 等. 不同种植密度对短葶山麦冬生长动态及产量质量的影响[J]. 中国农学通报, 2011, 27(27):226-230.
[18]	赵锐明, 孙连虎, 郭凤霞, 等. 移栽密度对红芪生长发育及药材产量和质量的影响[J]. 草业学报, 2014, 23(3):167-174.
[19]	PATANÈ C, COSENTINO S L, CALCAGNO S. How do sowing time and plant density affect the pigments safflomins and carthamin in florets of safflower[J]. Industrial crops and products, 2020. Doi: 10.1016/j.indcrop.2020.112313. doi: 10.1016/j.indcrop.2020.112313
[20]	张艳丽, 张重义, 李友军, 等. 牛膝不同密度群体光合速率与干物质积累和品质的关系[J]. 西北植物学报, 2008, 28(4):793-798.
[21]	王文颇, 李彦生, 周印富. 牛膝在不同种植密度下的生长动态规律[J]. 中国中药杂志, 2005, 30(14):1069-1072.
[22]	李庆会, 李志刚, 智燕凤, 等. 通辽地区甜菜最适种植密度分析[J]. 中国糖料, 2014(1):48-50.
[23]	周远航, 曾翠云, 王慧珍, 等. 种植密度对新疆红花采收部位籽粒品质的影响[J]. 新疆农业科学, 2020, 57(7):1195-1200. doi: 10.6048/j.issn.1001-4330.2020.07.003

栽培密度	取样时间（月/日）
栽培密度	7/17	7/28	8/8	8/25	9/10
D₁	0.23±0.02 Aa	0.31±0.01 Cc	0.54±0.01 Aa	0.54±0.01 Aa	0.54±0.04 Aa
D₂	0.25±0.02 Aa	0.40±0.02 abcABC	0.52±0.01 Aa	0.54±0.01 Aa	0.55±0.03 Aa
D₃	0.23±0.01 Aa	0.35±0.02 BCbc	0.53±0.01 Aa	0.54±0.03 Aa	0.53±0.04 Aa
D₄	0.25±0.02 Aa	0.43±0.03 ABab	0.54±0.02 Aa	0.55±0.02 Aa	0.53±0.02 Aa
D₅	0.21±0.03 Aa	0.47±0.02 Aa	0.57±0.02 Aa	0.59±0.04 Aa	0.58±0.03 Aa

栽培密度	取样时间（月/日）
栽培密度	7/17	7/28	8/8	8/25	9/10
D₁	0.23±0.02 Aa	0.31±0.01 Cc	0.54±0.01 Aa	0.54±0.01 Aa	0.54±0.04 Aa
D₂	0.25±0.02 Aa	0.40±0.02 abcABC	0.52±0.01 Aa	0.54±0.01 Aa	0.55±0.03 Aa
D₃	0.23±0.01 Aa	0.35±0.02 BCbc	0.53±0.01 Aa	0.54±0.03 Aa	0.53±0.04 Aa
D₄	0.25±0.02 Aa	0.43±0.03 ABab	0.54±0.02 Aa	0.55±0.02 Aa	0.53±0.02 Aa
D₅	0.21±0.03 Aa	0.47±0.02 Aa	0.57±0.02 Aa	0.59±0.04 Aa	0.58±0.03 Aa

栽培密度	取样时间（月/日）
栽培密度	7/17	7/28	8/8	8/25	9/10
D₁	18.98±0.78 Aa	30.93±1.04 Aa	46.92±2.10 Aa	52.80±2.87Ab	61.97±4.99 Bc
D₂	15.13±0.90 Aa	28.17±0.98Aa	47.90±2.97Aa	61.75±5.04Aa	63.98±3.69 Bb
D₃	19.62±1.12 Aa	28.63±1.49Aa	50.67±3.17 Aa	57.85±4.18Aa	71.52±2.01 Aa
D₄	18.10±0.86 Aa	32.83±1.76Aa	48.62±2.10 Aa	55.88±3.01Ab	65.58±3.69ABb
D₅	13.62±0.58 Aa	28.40±0.83Aa	49.83±1.54 Aa	58.88±3.05Aa	68.07±3.27Aab

栽培密度	取样时间（月/日）
栽培密度	7/17	7/28	8/8	8/25	9/10
D₁	18.98±0.78 Aa	30.93±1.04 Aa	46.92±2.10 Aa	52.80±2.87Ab	61.97±4.99 Bc
D₂	15.13±0.90 Aa	28.17±0.98Aa	47.90±2.97Aa	61.75±5.04Aa	63.98±3.69 Bb
D₃	19.62±1.12 Aa	28.63±1.49Aa	50.67±3.17 Aa	57.85±4.18Aa	71.52±2.01 Aa
D₄	18.10±0.86 Aa	32.83±1.76Aa	48.62±2.10 Aa	55.88±3.01Ab	65.58±3.69ABb
D₅	13.62±0.58 Aa	28.40±0.83Aa	49.83±1.54 Aa	58.88±3.05Aa	68.07±3.27Aab

栽培密度	取样时间（月/日）
栽培密度	7/17	7/28	8/8	8/25	9/10
D₁	18.37±0.01 Aa	23.73±0.30 Aa	26.40±0.70 Aa	31.03±0.17 Aa	31.73±0.3 Aa
D₂	15.03±0.01 Aa	22.60±0.21 Aa	26.70±0.50 Aa	28.37±0.73 Aab	28.17±0.94Aab
D₃	18.33±0.03 Aa	22.63±0.20 Aa	24.33±0.30Aab	29.10±0.25 Aab	29.73±0.94 Aab
D₄	18.20±0.02 Aa	32.83±0.73 Aa	23.67±0.33 Ab	25.60±1.58 Ab	27.10±0.38 Ab
D₅	17.60±0.04 Aa	23.03±0.46 Aa	26.73±0.77 Aa	27.87±1.17 Aab	27.23±1.32 Ab