Effects of Intensified Measures on Microbial Diversity in Copper Contaminated Soil

doi:10.11924/j.issn.1000-6850.casb2021-0550

Abstract

Abstract:

The effects of different intensified measures on microbial diversity in copper contaminated soil were explored to provide basic data and reference for explaining the mechanism of soil microorganisms affecting the absorption and accumulation of heavy metals by plants and improving the efficiency of plant-microbe combined remediation of heavy metal contaminated soil. Illumina-miseq high-throughput sequencing technology was used to sequence the V3-V4 region fragments of 16SrDNA gene under different intensified treatment measures in combination with field plot experiment, to reveal the composition and difference of microbial communities. The results show that a total of 42 phyla, 145 classes, 359 orders, 562 families, 1060 genera and 2290 species are identified in five different treatments. The composition of soil microbial community in different treatments is similar at phylum level, and the dominant species are same. The phyla with high abundance are Acidobacteria, Proteobacteria, Actinobacteria, Chloroflexi and Firmicutes. There are significant differences in soil microbial composition among different treatments at OTU level, and the order of effect on microbial community is copper stress > chelating agent > organic matter cover. Specific species appear in different treatments and the difference between blank control treatment and other treatments is the largest. The microbial community composition of copper stress treatment and organic matter cover treatment are the most similar. Cu stress increases the richness of soil microbial community. The diversity and richness of soil microorganisms are the highest under chelating agent treatment, and the difference of microbial community diversity between chelating agent treatment and organic matter cover treatment reaches a significant level.

Key words: copper contamination, chelating agent, organic matter cover, high-throughput sequencing

CLC Number:

S154.36

ZHANG Qian, ZHANG Guowei, SHANG Kankan. Effects of Intensified Measures on Microbial Diversity in Copper Contaminated Soil[J]. Chinese Agricultural Science Bulletin, 2022, 38(14): 96-103.

Figures/Tables 7

References 26

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序号	处理
CK	原土
T0	铜污染土壤
T1	铜污染土壤+6~8 cm树枝落叶类覆盖
T2	铜污染土壤+5 mmol/L EDTA
T3	铜污染土壤+6~8 cm树枝落叶类覆盖+5 mmol/L EDTA

序号	处理
CK	原土
T0	铜污染土壤
T1	铜污染土壤+6~8 cm树枝落叶类覆盖
T2	铜污染土壤+5 mmol/L EDTA
T3	铜污染土壤+6~8 cm树枝落叶类覆盖+5 mmol/L EDTA