Genetic Background Analysis of ‘Hetian’ Rose Based on DNA Sequence

doi:10.11924/j.issn.1000-6850.casb2022-0439

Abstract

Abstract:

‘Hetian’ rose is one of the main cultivated rose varieties in China, but its unclear genetic background is not conducive to product development and application. The suspected proximologous species of ‘Hetian’ rose were selected for this study, by using sequence alignment method and constructing the phylogenetic tree, we analyzed the genetic relationship of the varieties and clarified their genetic background. The results showed that the polymorphism of ITS sequence was high, especially ITS2 sequence, which could obtain more genetic information. Based on ITS2 sequence, ‘Hetian’ rose and Rosa gallica clustered into a small branch, indicating that their genetic relationship was close. The polymorphism of atpB-rbcL sequence was low among the tested plant materials, but it could reflect the maternal information. ‘Hetian’ rose was in the same branch with Rosa gallica, Rosa centifolia, Rosa phoenicia and Rosa rugosa. Combined with the clustering results of ITS, the study presumed Rosa gallica to be the female parent of ‘Hetian’ rose which had the common parental origin with Rosa damascena.

Key words: ‘Hetian’ rose, ITS, atpB-rbcL, clustering analysis, genetic relationship

CLC Number:

Q341

SONG Yao, WANG Hui. Genetic Background Analysis of ‘Hetian’ Rose Based on DNA Sequence[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 36-40.

Figures/Tables 5

References 25

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编号	种（品种）名	拉丁名或英文名	名称缩写
1	‘和田’玫瑰	‘Hetian’rose	ht
2	腓尼基蔷薇	Rosa phoenicia	fnj
3	麝香蔷薇	Rosa moschata	sx
4	法国蔷薇	Rosa gallica	gl
5	大马士革蔷薇	Rosa damascena	dm
6	百叶蔷薇	Rosa centifolia	by
7	大花香水月季	Rosa odorata var. gigantea	gig
8	‘月月粉’	R. chinensis ‘Old Blush’	yyf
9	‘墨红’	R. ‘Crimon Glory’	mh
10	‘滇红’	‘Dianhong’rose	dh
11	玫瑰	Rosa rugosa	mg
12	腺果蔷薇	Rosa fedtschenkoana	xg

编号	种（品种）名	拉丁名或英文名	名称缩写
1	‘和田’玫瑰	‘Hetian’rose	ht
2	腓尼基蔷薇	Rosa phoenicia	fnj
3	麝香蔷薇	Rosa moschata	sx
4	法国蔷薇	Rosa gallica	gl
5	大马士革蔷薇	Rosa damascena	dm
6	百叶蔷薇	Rosa centifolia	by
7	大花香水月季	Rosa odorata var. gigantea	gig
8	‘月月粉’	R. chinensis ‘Old Blush’	yyf
9	‘墨红’	R. ‘Crimon Glory’	mh
10	‘滇红’	‘Dianhong’rose	dh
11	玫瑰	Rosa rugosa	mg
12	腺果蔷薇	Rosa fedtschenkoana	xg

引物	序列(5'-3')		产物大小/bp	退火温度/℃	延伸时间/s
ITS	F	TCCTCCGCTTATTGATATGC	736~743	58	45
ITS	R	GGAAGTAAAAGTCGTAACAAGG	736~743	58	45
atpB-rbcL	F	GAAGTAGTAGGATTGATTCT	542~568	50	45
atpB-rbcL	R	CATCATTATTGTATACTCTTTC	542~568	50	45

引物	序列(5'-3')		产物大小/bp	退火温度/℃	延伸时间/s
ITS	F	TCCTCCGCTTATTGATATGC	736~743	58	45
ITS	R	GGAAGTAAAAGTCGTAACAAGG	736~743	58	45
atpB-rbcL	F	GAAGTAGTAGGATTGATTCT	542~568	50	45
atpB-rbcL	R	CATCATTATTGTATACTCTTTC	542~568	50	45