Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (28): 28-35.doi: 10.11924/j.issn.1000-6850.casb2021-0994
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WANG Chao1,2(), YANG Changyao1,3, LI Feifei4, MA Xiaochuan1,3, CHEN Yuewen1,3, XIAO Zhiwei5, YIN Tao1,3, YE Li1,3, LI Yunsong1,3, LU Xiaopeng1,3(
)
Received:
2021-10-20
Revised:
2021-12-17
Online:
2022-10-05
Published:
2022-09-28
Contact:
LU Xiaopeng
E-mail:3564037343@qq.com;x1678@hunau.edu.cn
CLC Number:
WANG Chao, YANG Changyao, LI Feifei, MA Xiaochuan, CHEN Yuewen, XIAO Zhiwei, YIN Tao, YE Li, LI Yunsong, LU Xiaopeng. Functional Identification of Citrus CsaccA Gene[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 28-35.
材料 | 根 | 茎 | 叶 | |||||
---|---|---|---|---|---|---|---|---|
鲜重 | 干重 | 鲜重 | 干重 | 鲜重 | 干重 | |||
野生型 | 0.65±0.32 | 0.11±0.02 | 3.60±0.75 | 0.68±0.24 | 7.37±1.80 | 0.73±0.14 | ||
转基因 | 6.87±2.05* | 0.44±0.27* | 17.28±4.03* | 4.05±1.13* | 24.01±8.55* | 2.65±1.13* |
材料 | 根 | 茎 | 叶 | |||||
---|---|---|---|---|---|---|---|---|
鲜重 | 干重 | 鲜重 | 干重 | 鲜重 | 干重 | |||
野生型 | 0.65±0.32 | 0.11±0.02 | 3.60±0.75 | 0.68±0.24 | 7.37±1.80 | 0.73±0.14 | ||
转基因 | 6.87±2.05* | 0.44±0.27* | 17.28±4.03* | 4.05±1.13* | 24.01±8.55* | 2.65±1.13* |
[1] |
YIN Dongmei, WANG Yun, ZHANG Xingguo, et al. De novo assembly of the peanut (Arachis hypogaea L.) seed transcriptome revealed candidate unigenes for oil accumulation pathways[J]. PLoS ONE, 2013,8(9):e73767.
doi: 10.1371/journal.pone.0073767 URL |
[2] |
LI S J, CRONAN J E. The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase[J]. Journal of biological chemistry, 1992,267(2):855-63.
doi: 10.1016/S0021-9258(18)48362-7 URL |
[3] | KONDO H, SHIRATSUCHI K, YOSHIMOTO T, et al. Acetyl-CoA carboxylase from Escherichia coli: gene organization and nucleotide sequence of the biotin carboxylase subunit[J]. Proceedings of the national academy of sciences of the United States of America, 1991,88(21):9730-9733. |
[4] |
ELBOROUGH K M, SIMON J W, SWINHOE R, et al. Studies on wheat acetyl CoA carboxylase and the cloning of a partial cDNA[J]. Plant molecular biology, 1994,24(1):21-34.
pmid: 7906561 |
[5] |
ASHTON A R, JENKINS C, WHITFELD P R. Molecular cloning of two different cDNAs for maize acetyl CoA carboxylase[J]. Plant molecular biology, 1994,24(1):35-49.
pmid: 7906562 |
[6] |
HARWOOD J L. Recent advances in the biosynthesis of plant fatty acids[J]. Biochimica et biophysica acta (BBA)- lipids and lipid metabolism, 1996,1301(1-2):7-56.
doi: 10.1016/0005-2760(95)00242-1 URL |
[7] |
GU Keyu, CHIAM Huihui, TIAN Dongsheng, et al. Molecular cloning and expression of heteromeric ACCase subunit genes from Jatropha curcas[J]. Plant science, 2011,180(4):642-649.
doi: 10.1016/j.plantsci.2011.01.007 pmid: 21421413 |
[8] |
KOZAKI A, MAYUMI K, SASAKI Y. Thiol-disulfide exchange between nuclear-encoded and chloroplast-encoded subunits of pea acetyl-CoA carboxylase[J]. Journal of biological chemistry, 2001,276(43):39919-25.
doi: 10.1074/jbc.M103525200 pmid: 11546765 |
[9] |
张煜星, 崔燕, 武寒雪. 等. CAC3基因转运肽序列和accD基因融合植物表达载体的构建[J]. 华北农学报, 2008(5):26-29.
doi: 10.7668/hbnxb.2008.05.006 |
[10] | 王保明. 油茶ACCase基因的克隆及功能研究[D]. 长沙: 中南林业科技大学, 2012:94-103. |
[11] | 杨婷, 张建平, 刘自刚. 等. 胡麻异质型ACCase亚基基因的克隆与表达分析[J]. 草业学报, 2020,29(4):111-120. |
[12] |
PLANK D W, GENGENBACH B G, GRONWALD J W. Effect of iron on activity of soybean multi-subunit acetyl-coenzyme A carboxylase[J]. Physiologia plantarum, 2010,112(2):183-194.
doi: 10.1034/j.1399-3054.2001.1120206.x URL |
[13] |
FOCKS N, BENNING C. wrinkled1: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism[J]. Plant physiology, 1998,118(1):91-101.
doi: 10.1104/pp.118.1.91 URL |
[14] | 杨青. 过表达乙酰辅酶A羧化酶以提高谷氨酸棒杆菌重组菌株中脂肪酸合成的研究[D]. 呼和浩特: 内蒙古农业大学, 2021:42. |
[15] |
赵爽, 刘洋, 李寒. 等. 早实核桃脂肪积累与酶活性动态及其相关性[J]. 应用生态学报, 2017,28(11):3521-3526.
doi: 10.13287/j.1001-9332.201711.007 |
[16] | 王哲. 油桐异质型ACCase基因的克隆及功能表达研究[D]. 长沙: 中南林业科技大学, 2015:88. |
[17] |
YE Yajin, NIKOVICS Krisztina, ALEXANDRA, et al. Docking of acetyl-CoA carboxylase to the plastid envelope membrane attenuates fatty acid production in plants[J]. Nature communications, 2020,11(1):6191.
doi: 10.1038/s41467-020-20014-5 pmid: 33273474 |
[18] | 崔燕. 棉花乙酰辅酶A羧化酶β亚基accD基因的克隆及在拟南芥中超量表达的研究[D]. 石河子: 石河子大学, 2008:28. |
[19] |
CHEN Duo, YUAN Xue, LIANG Limin, et al. Overexpression of acetyl-CoA carboxylase increases fatty acid production in the green alga Chlamydomonas reinhardtii[J]. Biotechnology letters, 2019,41(10):1133-1145.
doi: 10.1007/s10529-019-02715-0 URL |
[20] |
MAIARA Curtolo, MARIÂNGELA Cristofani-Yaly, RODRIGO Gazaffi, et al. QTL mapping for fruit quality in Citrus using DArTseq markers[J]. BMC Genomics, 2017,18(1):289.
doi: 10.1186/s12864-017-3629-2 pmid: 28403819 |
[21] |
LU Xiaopeng, CAO Xiongjun, LI Feifei, et al. Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis)[J]. Physiologia plantarum, 2016,158(4):463-482.
doi: 10.1111/ppl.12484 URL |
[22] | 王伏林, 吴关庭, 郎春秀. 等. 转异质型ACCase复合基因对油菜含油量的影响[J]. 分子植物育种, 2017,15(3):920-927. |
[23] |
KLAUS D, OHLROGGE J B, NEUHAUS H E, et al. Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase[J]. Planta, 2004,219(3):389-396.
pmid: 15014998 |
[24] |
DAVIS M S. Overproduction of Acetyl-CoA Carboxylase Activity Increases the Rate of Fatty Acid Biosynthesis in Escherichia coli[J]. Journal of biological chemistry, 2000,275(37):28593-28598.
doi: 10.1074/jbc.M004756200 URL |
[25] | SASAKI Y, NAGANO Y. Plant Acetyl-CoA Carboxylase: Structure, Biosynthesis, Regulation, and Gene Manipulation for Plant Breeding[J]. Journal of the agricultural chemical society of Japan, 2004,68(6):1175-1184. |
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