Functional Identification of Citrus CsaccA Gene

doi:10.11924/j.issn.1000-6850.casb2021-0994

Abstract

Abstract:

This study aims to improve the quality of citrus fruits. Function of a CsaccA gene, which is suspected to be related to citrus fruit quality, was systematically investigated through overexpression in Micro-Tom tomato, identification by PCR and GUS staining, plant phenotype observation and physiological and biochemical determinations. The results showed that the CsaccA gene significantly promoted plant biomass, and the biomass of the root, stem and leaf of transgenic plants were all significantly higher than that of control. The plant growth rate of transgenic plants was significantly faster than that of control as well. The transgenic fruit colored and ripened earlier than that of control. Quality analysis showed that the main sugar components of tomato fruit, glucose and fructose, had a significant increase in content. However, the main acid component citrate had a decrease in content significantly. In summary, transgenic studies have verified that the CsaccA gene functions in increasing fruit sugar-to-acid ratio, which can lay a foundation for fruit quality improvement in the future.

Key words: citrus, acetyl-CoA carboxylase, CsaccA gene, tomato, transgene, sugar, acid

CLC Number:

S666.01

WANG Chao, YANG Changyao, LI Feifei, MA Xiaochuan, CHEN Yuewen, XIAO Zhiwei, YIN Tao, YE Li, LI Yunsong, LU Xiaopeng. Functional Identification of Citrus CsaccA Gene[J]. Chinese Agricultural Science Bulletin, 2022, 38(28): 28-35.

Figures/Tables 5

References 25

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材料	根		茎		叶
材料	鲜重	干重	鲜重	干重	鲜重	干重
野生型	0.65±0.32	0.11±0.02	3.60±0.75	0.68±0.24	7.37±1.80	0.73±0.14
转基因	6.87±2.05^*	0.44±0.27^*	17.28±4.03^*	4.05±1.13^*	24.01±8.55^*	2.65±1.13^*

材料	根		茎		叶
材料	鲜重	干重	鲜重	干重	鲜重	干重
野生型	0.65±0.32	0.11±0.02	3.60±0.75	0.68±0.24	7.37±1.80	0.73±0.14
转基因	6.87±2.05^*	0.44±0.27^*	17.28±4.03^*	4.05±1.13^*	24.01±8.55^*	2.65±1.13^*