Effect of Heterotrophic AOB on the Growth-promoting of Lolium multiflorum

doi:10.11924/j.issn.1000-6850.casb2022-0974

Abstract

Abstract:

This study aimed to elucidate the growth-promoting effects of Ammonia oxidizing bacteria (AOB) strains on Italian ryegrass (Lolium multiflorum Lam.). Pot-grown Italian ryegrass was used as the experimental material, four treatments were established: no nitrification inhibitor and no inoculation, inoculation with AOB strains after regeneration, regeneration after adding a nitrification inhibitor, and inoculation with AOB strains after adding a nitrification inhibitor. The results showed that, compared to the non-inhibited and non-inoculated regeneration, Italian ryegrass inoculated with AOB strains exhibited an increase of 23.53% to 48.63% in leaf biomass, 27.91% to 48.54% in net photosynthetic rate, and 43.01% to 144.21% in rhizosphere soil nitrification rate. The addition of a nitrification inhibitor in the soil suppressed the growth promotion of Italian ryegrass. However, after inoculating with the S2_8_1 strain, the growth-promoting effects were superior to those of adding a nitrification inhibitor. In conclusion, the inoculation of S2_8_1 enhanced the nitrification process in Italian ryegrass rhizosphere, stimulated the increase of NO₃^- content, and strengthened photosynthesis, and ultimately promoted the growth-promoting effect of Italian ryegrass.

Key words: Ammonia oxdizing bacteria, rhizosphere, soil nitrification, photosynthetic rate, regeneration

LI Ya’nan, WANG Haihong, WU Di, LI Lixiang, ZHU Xixia, QI Lin, ZHANG Mingming, WANG Xiaoling. Effect of Heterotrophic AOB on the Growth-promoting of Lolium multiflorum[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 157-164.

Figures/Tables 4

References 36

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指标	再生天数/d	处理
指标	再生天数/d	RT	RI	RN	RNI
根际土壤硝化速率/[mg/(kg·d)]	0	2.73a±0.23	2.44a±0.30	1.22b±0.09	1.57b±0.12
	6	2.00b±0.21	2.86a±0.38	1.38c±0.24	0.95c±0.08
	12	0.95b±0.04	2.32a±0.09	0.16c±0.03	0.22c±0.02
非根际土壤硝化速/[mg/(kg·d)]	0	1.17a±0.20	1.29a±0.12	0.72b±0.12	0.65b±0.08
	6	1.33b±0.14	1.76a±0.21	0.57c±0.07	0.70c±0.08
	12	0.88b±0.10	1.80a±0.13	0.12c±0.01	0.17c±0.02
根际土壤NO₃^-含量/(mg/kg)	0	12.84a±1.04	11.37a±1.21	13.21a±1.99	12.54a±0.61
	6	8.81b±0.54	11.60a±1.11	7.13c±0.40	7.23c±0.57
	12	7.44b±0.30	11.99a±0.97	4.90c±0.31	5.64c±0.83
非根际土壤NO₃^-含量(mg/kg)	0	8.09bc±0.71	10.97a±0.78	7.89c±0.46	9.46b±0.92
	6	9.55ab±1.05	6.58c±0.87	9.82a±1.05	7.94bc±0.65
	12	6.83b±0.68	8.68a±0.98	3.51d±0.28	5.49c±0.52
根际土壤NH₄⁺含量/(mg/kg)	0	10.56a±0.90	12.08a±0.40	11.57a±0.90	11.46a±0.84
	6	4.76b±0.53	5.45b±0.71	8.47a±1.30	9.74a±0.15
	12	9.59b±0.04	8.56b±0.35	11.10ab±2.57	12.67a±0.68
非根际土壤NH₄⁺含量/(mg/kg)	0	8.29a±0.71	9.50a±0.31	9.27a±0.72	8.56a±0.63
	6	5.12c±0.57	6.08bc±0.79	6.72ab±1.03	8.01a±0.13
	12	7.12b±0.38	6.96b±0.28	9.78a±1.34	10.89a±0.58

指标	再生天数/d	处理
指标	再生天数/d	RT	RI	RN	RNI
根际土壤硝化速率/[mg/(kg·d)]	0	2.73a±0.23	2.44a±0.30	1.22b±0.09	1.57b±0.12
	6	2.00b±0.21	2.86a±0.38	1.38c±0.24	0.95c±0.08
	12	0.95b±0.04	2.32a±0.09	0.16c±0.03	0.22c±0.02
非根际土壤硝化速/[mg/(kg·d)]	0	1.17a±0.20	1.29a±0.12	0.72b±0.12	0.65b±0.08
	6	1.33b±0.14	1.76a±0.21	0.57c±0.07	0.70c±0.08
	12	0.88b±0.10	1.80a±0.13	0.12c±0.01	0.17c±0.02
根际土壤NO₃^-含量/(mg/kg)	0	12.84a±1.04	11.37a±1.21	13.21a±1.99	12.54a±0.61
	6	8.81b±0.54	11.60a±1.11	7.13c±0.40	7.23c±0.57
	12	7.44b±0.30	11.99a±0.97	4.90c±0.31	5.64c±0.83
非根际土壤NO₃^-含量(mg/kg)	0	8.09bc±0.71	10.97a±0.78	7.89c±0.46	9.46b±0.92
	6	9.55ab±1.05	6.58c±0.87	9.82a±1.05	7.94bc±0.65
	12	6.83b±0.68	8.68a±0.98	3.51d±0.28	5.49c±0.52
根际土壤NH₄⁺含量/(mg/kg)	0	10.56a±0.90	12.08a±0.40	11.57a±0.90	11.46a±0.84
	6	4.76b±0.53	5.45b±0.71	8.47a±1.30	9.74a±0.15
	12	9.59b±0.04	8.56b±0.35	11.10ab±2.57	12.67a±0.68
非根际土壤NH₄⁺含量/(mg/kg)	0	8.29a±0.71	9.50a±0.31	9.27a±0.72	8.56a±0.63
	6	5.12c±0.57	6.08bc±0.79	6.72ab±1.03	8.01a±0.13
	12	7.12b±0.38	6.96b±0.28	9.78a±1.34	10.89a±0.58