Abstract: The sensitivity of 60 isolates obtained from the blight stems of rape in Anhui province to carbendazim were tested in vitro. The results showed that 18 carbendazim-resistance isolates were found among them from 10 localities in Anhui province, but the proportion of the carbendazim-resistance isolates from 10 localities varied with the highest ratio 66.7% in Mongcheng and the lowest 9.1% in Taihu. The results also indicated that THS-1 obtained from Taihu has the highest resistance to carbendazim, with the EC50 value 743.8347mg/L. It suggested that the pathogen causing rape sclerotiniose in Anhui province had developed high resistance to carbendazim.