Wild Soybean Germplasm Resources in Cold Region: Free Amino Acid Components Analysis

doi:10.11924/j.issn.1000-6850.casb2023-0027

Abstract

Abstract:

This study aimed to analyze the free amino acid components of wild soybean resources which were collected from various regions of Heilongjiang Province. 17 kinds of free amino acid content in 153 wild soybean resources were determined and analyzed systematically. The results indicated that the amplitude variation of methionine content was the highest among the 17 kinds of free amino acids with a variation coefficient of 0.469. The total free amino acid content was 2.069 mg/g, among which the arginine content was the highest (0.894 mg/g). Positive correlation was showed between any free amino acids and total free amino acids. There were significant positive correlations among most amino acids, while significant negative correlation could be found between aspartic acid and phenylalanine and between serine and arginine. The cumulative contribution rate of the first five principal components extracted from the principal component analysis reached 85.15%. The contribution rates of the first and second principal components were 26.47% and 20.55%, respectively. According to the first and second principal components, large discrepancies were discovered between the resources in the northern region and the central and southern regions, and small discrepancies were discovered between the resources in the eastern and western regions. In this study, the content variation and correlation among components of 17 kinds of free amino acids in wild soybean in cold region were analyzed, and the effects of different sources on the content and components of free amino acids were clarified, which provided important basic data for soybean breeding applications.

Key words: wild soybean in cold region, free amino acid, variation coefficient, correlation analysis, principal component analysis

FAN Chao, BI Yingdong, LIU Miao, LI Wei, LIU Jianxin, YANG Guang, LIANG Wenwei, DI Shufeng, TANG Lili, LAI Yongcai. Wild Soybean Germplasm Resources in Cold Region: Free Amino Acid Components Analysis[J]. Chinese Agricultural Science Bulletin, 2024, 40(12): 134-141.

Figures/Tables 6

References 26

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所属生态区	采集地所属城市	采集地所属县（区）数	份数
西部地区	大庆	5	10
西部地区	齐齐哈尔	8	17
东部地区	佳木斯	7	17
	双鸭山	5	11
	七台河	3	5
	鸡西	3	11
	牡丹江	6	10
中南部地区	绥化	7	18
中南部地区	哈尔滨	14	26
北部地区	黑河	5	15
北部地区	伊春	7	13
合计		70	153

所属生态区	采集地所属城市	采集地所属县（区）数	份数
西部地区	大庆	5	10
西部地区	齐齐哈尔	8	17
东部地区	佳木斯	7	17
	双鸭山	5	11
	七台河	3	5
	鸡西	3	11
	牡丹江	6	10
中南部地区	绥化	7	18
中南部地区	哈尔滨	14	26
北部地区	黑河	5	15
北部地区	伊春	7	13
合计		70	153

氨基酸	平均值/(mg/g)	最大值/(mg/g)	最小值/(mg/g)	标准差	变异系数
丙氨酸	0.057	0.098	0.03	0.018	0.204
精氨酸	0.894	1.206	0.724	0.104	0.116
天冬氨酸	0.228	0.440	0.100	0.062	0.272
半胱氨酸	0.236	0.360	0.021	0.044	0.188
谷氨酸	0.248	0.508	0.108	0.065	0.261
甘氨酸	0.016	0.030	0.001	0.007	0.417
组氨酸	0.116	0.314	0.046	0.039	0.334
异亮氨酸	0.035	0.103	0.006	0.013	0.419
亮氨酸	0.026	0.044	0.007	0.008	0.300
赖氨酸	0.029	0.049	0.013	0.008	0.262
甲硫氨酸	0.025	0.079	0.002	0.012	0.469
苯丙氨酸	0.057	0.103	0.034	0.012	0.214
脯氨酸	0.023	0.048	0.006	0.009	0.400
丝氨酸	0.018	0.037	0.003	0.008	0.441
苏氨酸	0.019	0.036	0.005	0.007	0.377
酪氨酸	0.026	0.063	0.008	0.008	0.314
缬氨酸	0.027	0.045	0.010	0.008	0.307
总氨基酸	2.069	2.680	1.347	0.192	0.093

氨基酸	平均值/(mg/g)	最大值/(mg/g)	最小值/(mg/g)	标准差	变异系数
丙氨酸	0.057	0.098	0.03	0.018	0.204
精氨酸	0.894	1.206	0.724	0.104	0.116
天冬氨酸	0.228	0.440	0.100	0.062	0.272
半胱氨酸	0.236	0.360	0.021	0.044	0.188
谷氨酸	0.248	0.508	0.108	0.065	0.261
甘氨酸	0.016	0.030	0.001	0.007	0.417
组氨酸	0.116	0.314	0.046	0.039	0.334
异亮氨酸	0.035	0.103	0.006	0.013	0.419
亮氨酸	0.026	0.044	0.007	0.008	0.300
赖氨酸	0.029	0.049	0.013	0.008	0.262
甲硫氨酸	0.025	0.079	0.002	0.012	0.469
苯丙氨酸	0.057	0.103	0.034	0.012	0.214
脯氨酸	0.023	0.048	0.006	0.009	0.400
丝氨酸	0.018	0.037	0.003	0.008	0.441
苏氨酸	0.019	0.036	0.005	0.007	0.377
酪氨酸	0.026	0.063	0.008	0.008	0.314
缬氨酸	0.027	0.045	0.010	0.008	0.307
总氨基酸	2.069	2.680	1.347	0.192	0.093

	因子1(PC1)	因子2(PC2)	因子3(PC3)	因子4(PC4)	因子5(PC5)
丙氨酸	-0.3364	0.0680	-0.0002	0.4551	-0.1155
精氨酸	-0.1236	0.1686	0.5393	0.0396	-0.0718
天冬氨酸	-0.2181	0.1668	0.2264	-0.4289	-0.0839
半胱氨酸	-0.0523	0.1028	0.1694	0.4987	0.2569
谷氨酸	-0.5060	0.1941	0.0110	0.2864	0.1578
甘氨酸	-0.1225	-0.2973	0.1823	0.0646	0.5103
组氨酸	-0.1812	-0.1445	-0.3164	-0.0265	0.4653
异亮氨酸	-0.5143	0.0547	-0.0372	-0.3747	0.0428
亮氨酸	-0.3907	0.0229	0.1183	-0.2199	-0.0243
赖氨酸	-0.1347	-0.2399	-0.1980	-0.1578	-0.1717
甲硫氨酸	-0.4547	-0.0318	-0.3385	0.0807	-0.0266
苯丙氨酸	-0.0473	-0.5118	-0.2906	0.0663	-0.0444
脯氨酸	-0.1932	0.1846	0.0158	0.2957	-0.4814
丝氨酸	-0.1399	-0.3477	0.4297	0.3246	-0.0570
苏氨酸	-0.1103	-0.3235	0.1643	0.0819	-0.2752
酪氨酸	0.1086	-0.4816	-0.0390	-0.0801	-0.2476
缬氨酸	-0.3172	-0.0553	0.3234	-0.2964	-0.0008
特征值	2.9731	2.4013	1.5719	1.3405	1.0880
贡献率/%	26.47	20.55	16.52	13.65	7.96
累计贡献率/%	26.47	47.02	63.54	77.19	85.15