Analysis of Volatile Components of Endophytic Fungus HDM07 in Cannabis sativa L.

doi:10.11924/j.issn.1000-6850.casb2024-0055

Abstract

Abstract:

This study aims to exploit volatile components of endophytic fungus HDM07 isolated from Cannabis sativa L., and to explore the biosynthetic pathway of secondary metabolites from HDM07. The research took the endophytic fungus HDM07 obtained from Cannabis sativa L. as the subject. GC-MS and retrieval mass spectrometry database were employed to identify the volatile components in both the fermentation broth and mycelium of HDM07. Morphological observation and 18S rDNA sequence analysis were conducted for strain identification. Results indicated that the method of GC-MS and mass spectrometry analysis, along with database retrieval from endophytic fungi HDM07, determined 11 volatile components in the fermented liquid. Furthermore, fungus HDM07 revealed 14 volatile constituents in mycelium and Cannabis sativa L. leaf identified 12 elements. In a blank reference substance solution (PDB medium), appraisal revealed 7 ingredients. The endophytic fungus HDM07 was identified as Alternaria alternate. These findings suggest that the synthesis pathways of volatile components in HDM07 fermentation broth and mycelia are both related yet distinct. Both the fermentation broth and mycelium of endophytic fungus HDM07 contained similar or identical volatile components to those found in Cannabis sativa L., and also contained unique ones, indicating a certain correlation between the strain and its host plant's synthesis of volatile components.

Key words: Cannabis sativa L., endophytic fungus HDM07 from Cannabis sativa L., 18S rDNA sequence analysis, GC-MS, volatile component

XING Xiangyu, CONG Tianyue, GUAN Xin, QI Kexiang, LI Wanru, ZHANG He, YOU Mengyao, WU Tong, ZHENG Chunying. Analysis of Volatile Components of Endophytic Fungus HDM07 in Cannabis sativa L.[J]. Chinese Agricultural Science Bulletin, 2024, 40(30): 103-113.

Figures/Tables 13

References 33

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仪器	型号规格	生产厂家
电子精密天平	PL303	梅特勒-托利多仪器有限公司
超净工作台	SZX	南通科学仪器有限公司
空气浴振荡器	HZQ-C	哈尔滨市东联电子技术开发有限公司
旋转蒸发仪	R-201	上海申胜生物技术有限公司
超声波清洗器	KQ-100DE	昆山市超声仪器有限公司
紫外可见波长检测器	FL2000	浙江温岭福立分析仪器有限公司
高效液相色谱仪	FL2000	浙江温岭福立分析仪器有限公司

仪器	型号规格	生产厂家
电子精密天平	PL303	梅特勒-托利多仪器有限公司
超净工作台	SZX	南通科学仪器有限公司
空气浴振荡器	HZQ-C	哈尔滨市东联电子技术开发有限公司
旋转蒸发仪	R-201	上海申胜生物技术有限公司
超声波清洗器	KQ-100DE	昆山市超声仪器有限公司
紫外可见波长检测器	FL2000	浙江温岭福立分析仪器有限公司
高效液相色谱仪	FL2000	浙江温岭福立分析仪器有限公司

序号	分子式	化合物名称	相对含量/%	保留时间/min
1	C₄H₆S	硫化二乙烯	0.81	12.34
2	C₈H₁₀O₂	对羟基苯乙醇	1.40	27.99
3	C₂₄H₃₉FO₂	邻氟苯甲酸十七烷基酯	0.63	53.51
4	C₁₀H₁₄O₂	3-异丙氧基-5-甲基苯酚	0.16	80.17
5	C₂₆H₄₂O₄	丁基十四烷酞酸酯	0.04	92.28
6	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	3.50	104.20
7	C₂₇H₄₂O₄	2-(3-乙酰氧基-4,4,14-三甲基雄甾-8-烯-17-基)-丙酸	0.04	115.39
8	C₂₂H₄₂O₄	己二酸二辛酯	6.43	140.00
9	C₂₂H₄₃NO	芥酸酰胺	0.69	181.47
10	C₂₈H₄₀O	麦角甾-4,6,8(14),22-四烯-3-酮	0.08	212.19
11	C₃₅H₆₂O₃	3,5-二(1,1-二甲基乙基)-4-羟基-苯丙酸十八烷基酯	0.51	228.79

序号	分子式	化合物名称	相对含量/%	保留时间/min
1	C₄H₆S	硫化二乙烯	0.81	12.34
2	C₈H₁₀O₂	对羟基苯乙醇	1.40	27.99
3	C₂₄H₃₉FO₂	邻氟苯甲酸十七烷基酯	0.63	53.51
4	C₁₀H₁₄O₂	3-异丙氧基-5-甲基苯酚	0.16	80.17
5	C₂₆H₄₂O₄	丁基十四烷酞酸酯	0.04	92.28
6	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	3.50	104.20
7	C₂₇H₄₂O₄	2-(3-乙酰氧基-4,4,14-三甲基雄甾-8-烯-17-基)-丙酸	0.04	115.39
8	C₂₂H₄₂O₄	己二酸二辛酯	6.43	140.00
9	C₂₂H₄₃NO	芥酸酰胺	0.69	181.47
10	C₂₈H₄₀O	麦角甾-4,6,8(14),22-四烯-3-酮	0.08	212.19
11	C₃₅H₆₂O₃	3,5-二(1,1-二甲基乙基)-4-羟基-苯丙酸十八烷基酯	0.51	228.79

序号
1	C₁₂H₃₆O₆Si₆	十二甲基环六硅氧烷	1.32	17.13
2	C₁₄H₄₂O₇Si₇	十四甲基环七硅氧烷	0.78	32.52
3	C₂₄H₃₉FO₂	邻氟苯甲酸十七烷基酯	1.26	53.51
4	C₁₈H₃₆O	十八烷醛	0.29	69.13
5	C₁₆H₂₂O₄	邻苯二甲酸二丁酯	3.69	104.14
6	C₁₆H₃₂O₂	棕榈酸	1.13	111.93
7	C₁₈H₃₅NO	油酸酰胺	0.55	137.29
8	C₂₀H₆₀O₁₀Si₁₀	二十甲基环十硅氧烷	0.50	150.60
9	C₂₁H₃₈O₄	顺9,12-十八碳二烯酸-2,3-二羟基丙基酯	0.43	171.02
10	C₃₀H₅₀	2,6,10,15,19,23-六甲基-2,6,10,14,18,22-二十四碳六烯	1.37	186.14
11	C₂₈H₄₂O	脱氢麦角甾醇	1.81	193.35
12	C₂₈H₄₄O	麦角甾醇	1.45	205.00
13	C₂₈H₄₀O	麦角甾-4,6,8(14),22-四烯-3-酮	0.40	212.19
14	C₃₅H₆₂O₃	3,5-二(1,1-二甲基乙基)-4-羟基-苯丙酸十八烷基酯	0.10	228.79