Effect of Density on the Survival of Megalocaria dilatata in Different Stages

doi:10.11924/j.issn.1000-6850.casb2024-0530

Abstract

Abstract:

In order to clarify the effects of rearing with different volume-density combinations on the developmental duration, survival rate, and weight of pupae and adults of the ten-spotted ladybird beetles, under conditions of 25℃, relative humidity of 60% to 80%, and a light cycle of 10 L:14 D, three containers (A: 1000 mL; B: 1500 mL; C: 2000 mL) and 3 densities (15 heads; 20 heads; 25 heads), feeding, recording and analyzing the development period, survival rate, pupal and adult weight of each insect of M. dilatata. The results showed that the survival curve of M. dilatata showed an "S" downward trend with the growth of instar, and the development duration of each treatment was C₃>C₁>A₁=C₂>A₃>A₂=B₂>B₁>B₃, and the average development time of group B was the shortest. The survival rate of the 1st and 2nd instar larval stage and pupal stage of each treatment was lower, and the pupation rate and adult rate of B₂ treatment were 76.67% and 73.33%, which were higher than those of the other treatments. The weight of each treatment at pupal stage from high to low was A₁>C₂>C₃>B₁>A₃>C₁>B₃>A₂>B₂, and the weight of adults from high to low was B₁>B₃>B₂>C₂>C₃>C₁>A₂>A₃>A₁. In summary, the best feeding container for M. dilatata should be B₂ treatment, that is, 1500 mL feeding density of 20 heads/box is the best. This study provides a theoretical basis for the efficient breeding of larvae of M. dilatata.

Key words: aphid, Megalocaria dilatata, larva, density, biological control, volume-density combinations, developmental duration, survival rate, weight

LIANG Hui, DENG Quan, LIU Guo, CHEN Hezhu, MA Peng, LI Bin, LIU Dongyang, YU Jiamin, JIANG Lianqiang, PU Deqiang. Effect of Density on the Survival of Megalocaria dilatata in Different Stages[J]. Chinese Agricultural Science Bulletin, 2025, 41(8): 105-110.

Figures/Tables 6

References 28

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处理/mL		1龄/d	2龄/d	3龄/d	4龄/d	蛹期/d	合计/d
A	A₁	3.00±0.00ab	2.00±0.00a	2.00±0.00a	5.00±0.00bc	8.00±1.00a	20.00
	A₂	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	8.00±1.41a	19.67
	A₃	3.33±0.58a	2.00±0.00a	2.00±0.00a	4.33±1.15c	8.33±0.58a	19.99
B	B₁	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.33±0.58c	8.33±0.58a	19.66
	B₂	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	8.00±1.00a	19.67
	B₃	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	7.67±0.58a	19.34
C	C₁	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.67±1.53a	7.00±1.00a	20.34
	C₂	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.33±0.58ab	7.00±0.00a	20.00
	C₃	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.33±0.58ab	8.50±0.71a	21.50

处理/mL		1龄/d	2龄/d	3龄/d	4龄/d	蛹期/d	合计/d
A	A₁	3.00±0.00ab	2.00±0.00a	2.00±0.00a	5.00±0.00bc	8.00±1.00a	20.00
	A₂	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	8.00±1.41a	19.67
	A₃	3.33±0.58a	2.00±0.00a	2.00±0.00a	4.33±1.15c	8.33±0.58a	19.99
B	B₁	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.33±0.58c	8.33±0.58a	19.66
	B₂	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	8.00±1.00a	19.67
	B₃	3.00±0.00ab	2.00±0.00a	2.00±0.00a	4.67±0.58c	7.67±0.58a	19.34
C	C₁	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.67±1.53a	7.00±1.00a	20.34
	C₂	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.33±0.58ab	7.00±0.00a	20.00
	C₃	2.67±0.58b	2.00±0.00a	2.00±0.00a	6.33±0.58ab	8.50±0.71a	21.50

处理/mL		1—2龄	3龄	4龄	蛹期	羽化
A	A₁	86.67±17.64ab	83.17±11.82a	97.44±4.44a	97.22±4.81ab	61.94±28.28a
	A₂	75.00±10.00b	83.94±6.13a	95.21±4.18a	97.62±4.12ab	52.99±47.65a
	A₃	81.33±6.11ab	79.67±21.94a	92.48±8.45a	91.07±2.33b	60.31±43.43a
B	B₁	86.67±6.67ab	84.52±13.52a	100.00±0.00a	97.44±4.44ab	85.86±17.23a
	B₂	95.00±5.00a	81.96±14.30a	97.92±3.61a	100.00±0.00a	95.94±3.36a
	B₃	90.00±4.62ab	80.81±11.57a	93.33±5.77a	98.15±3.21ab	90.15±2.77a
C	C₁	86.67±6.67ab	92.86±12.37a	94.41±4.90a	96.67±5.77ab	40.74±12.83a
	C₂	86.67±5.77ab	88.89±14.70a	95.83±7.22a	95.48±4.02ab	67.56±41.04a
	C₃	89.33±9.24ab	79.37±19.25a	98.67±2.31a	93.27±6.31ab	34.72±33.42a

处理/mL		1—2龄	3龄	4龄	蛹期	羽化
A	A₁	86.67±17.64ab	83.17±11.82a	97.44±4.44a	97.22±4.81ab	61.94±28.28a
	A₂	75.00±10.00b	83.94±6.13a	95.21±4.18a	97.62±4.12ab	52.99±47.65a
	A₃	81.33±6.11ab	79.67±21.94a	92.48±8.45a	91.07±2.33b	60.31±43.43a
B	B₁	86.67±6.67ab	84.52±13.52a	100.00±0.00a	97.44±4.44ab	85.86±17.23a
	B₂	95.00±5.00a	81.96±14.30a	97.92±3.61a	100.00±0.00a	95.94±3.36a
	B₃	90.00±4.62ab	80.81±11.57a	93.33±5.77a	98.15±3.21ab	90.15±2.77a
C	C₁	86.67±6.67ab	92.86±12.37a	94.41±4.90a	96.67±5.77ab	40.74±12.83a
	C₂	86.67±5.77ab	88.89±14.70a	95.83±7.22a	95.48±4.02ab	67.56±41.04a
	C₃	89.33±9.24ab	79.37±19.25a	98.67±2.31a	93.27±6.31ab	34.72±33.42a

处理/mL		蛹期/mg	成虫/mg
A	A₁	188.40±13.00aA	120.60±5.50eC
	A₂	164.60±9.10 bB	137.00±5.20deBC
	A₃	172.50±15.40abAB	132.70±5.30deC
B	B₁	174.60±7.60abAB	167.10±15.80aA
	B₂	163.00±4.70 bB	161.90±8.60abAB
	B₃	166.40±7.00 bAB	163.00±6.50abA
C	C₁	172.40±2.80abAB	142.60±14.30cdABC
	C₂	175.30±6.50abAB	157.90±12.80abcAB
	C₃	174.90±3.40abAB	145.50±6.80bcdABC