Abstract: The tissue culture and rapid propagation of Prunus lannesiana Wils was studied in this paper.The results showed that the optimum medium for propagation was MS/ML + 6-BA0.5mg/L + NAA 0.05mg/L + sugar 40g/L + agar 4.5 g/L, and the ratio of proliferation was 2.47~2.56;Shoots were rooted on the medium ML + IBA 0.05 mg/L + NAA 0.05 mg/L +sugar20g/L +agar4.5g/L,and the rooting rate was 100%;Being acclimatized for 12~17d,the rooted plantlets were transplanted to turfy-soil ,the survival rate was 90%.