Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192

doi:10.11924/j.issn.1000-6850.casb2024-0338

Abstract

Abstract:

The effects of different carbon sources on the mycelium growth and extracellular enzyme activities of A15 and W192 were investigated to understand the physiological requirements of carbohydrates of the two commercial Agaricus bisporus strains. Using different monosaccharides, disaccharides, oligosaccharides and polysaccharides as test materials, the mycelial growth rate, mycelial biomass and extracellular lignocellulase activities were determined. Glucose and fructose increased the mycelial biomass of A15 by 7.7%-30.8% and 23.1%-38.5%, respectively, while that of W192 was increased by 21.1%-36.8% and 26.3%-57.9%, respectively. Trehalose, sucrose, starch and cellulose increased the growth rate of mycelia; trehalose, maltose, sucrose, xylo-oligosaccharide, starch and cellulose increased mycelial biomass, among which 0.5% cellulose increased the mycelial biomass of A15 and W192 by 3.3 and 2.3 times, respectively. 10 carbon sources increased laccase, C1 and Cx activities of A15 and W192, respectively. All the 12 carbon sources inhibited β-GC activity of A15. However, in addition to xylan and lignin, the β-GC activity of W192 was increased. Trehalose, maltose, sucrose, xylo-oligosaccharide, starch and microcrystalline cellulose promote mycelial growth significantly. W192 may have higher carbon source demand or tolerance than A15.

Key words: Agaricus bisporus, commercial stains, A15, W192, carbon source, mycelial growth, extracellular enzyme

WANG Qian, XIAO Tingting, ZHANG Yuchen, ZHANG Jinjing, JUAN Jiaxiang, CHEN Hui, CHEN Mingjie, HUANG Jianchun. Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192[J]. Chinese Agricultural Science Bulletin, 2025, 41(9): 140-148.

Figures/Tables 6

References 27

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碳源	A15
碳源	0.50%	1%	2%	4%
葡萄糖	1.65±0.07 n	1.48±0.06 o	1.42±0.05 op	1.21±0.07 qr
木糖	1.91±0.14 jk	1.40±0.05 op	1.34±0.09 p	0.94±0.11 s
果糖	1.69±0.05 mn	1.43±0.08 op	1.36±0.03 op	1.48±0.08 o
海藻糖	2.07±0.13 hi	2.41±0.15 bcde	2.42±0.08 bcde	2.18±0.34 gh
纤维二糖	1.62±0.22 n	1.35±0.07 op	1.32±0.06 pq	1.17±0.06 r
麦芽糖	2.09±0.17 hi	2.29±0.19 efg	2.23±0.09 fg	1.79±0.25 klm
蔗糖	2.39±0.10 cde	2.59±0.13 a	2.42±0.13 bcde	2.39±0.40 cde
低聚木糖	2.29±0.12 efg	2.35±0.13 def	1.74±0.04 lmn	2.05±0.07 i
淀粉	2.41±0.08 bcde	2.52±0.14 abc	2.54±0.14 ab	2.22±0.25 fg
纤维素	2.59±0.12 a	2.40±0.18 cde	2.47±0.09 abcd	2.57±0.22 a
木聚糖	1.98±0.17 ij	1.86±0.06 jkl	1.75±0.06 lmn
木质素	2.18±0.17 gh	1.69±0.11 mn
CK	2.34±0.10 def
碳源	W192
碳源	0.50%	1%	2%	4%
葡萄糖	2.89±0.10 ijk	3.09±0.06 def	3.00±0.09 gh	2.07±0.05 s
木糖	3.07±0.14 efg	2.90±0.03 ij	2.58±0.05 op	1.76±0.05 t
果糖	2.58±0.06 op	2.59±0.07 op	2.73±0.11 mn	2.16±0.15 r
海藻糖	3.09±0.05 def	3.18±0.08 bc	3.17±0.06 bcd	3.23±0.04 ab
纤维二糖	2.81±0.10 klm	2.74±0.10 mn	2.32±0.08 q	1.54±0.07 u
麦芽糖	3.09±0.10 def	3.19±0.08 bc	3.06±0.11 efg	2.95±0.08 hi
蔗糖	3.17±0.11 bcd	3.11±0.10 cdef	3.10±0.06 cdef	3.29±0.07 a
低聚木糖	2.97±0.14 hi	3.12±0.04 cde	2.83±0.07 jkl	2.54±0.11 p
淀粉	3.14±0.13 cde	3.03±0.07 fgh	2.91±0.10 ij	2.78±0.12 lm
纤维素	3.11±0.08 cdef	3.16±0.06 bcd	2.97±0.16 hi	2.86±0.11 jkl
木聚糖	2.66±0.09 no	2.66±0.08 no	2.13±0.08 rs
木质素	2.66±0.12 no	2.34±0.18 q
CK	3.10±0.04 cdef

碳源	A15
碳源	0.50%	1%	2%	4%
葡萄糖	1.65±0.07 n	1.48±0.06 o	1.42±0.05 op	1.21±0.07 qr
木糖	1.91±0.14 jk	1.40±0.05 op	1.34±0.09 p	0.94±0.11 s
果糖	1.69±0.05 mn	1.43±0.08 op	1.36±0.03 op	1.48±0.08 o
海藻糖	2.07±0.13 hi	2.41±0.15 bcde	2.42±0.08 bcde	2.18±0.34 gh
纤维二糖	1.62±0.22 n	1.35±0.07 op	1.32±0.06 pq	1.17±0.06 r
麦芽糖	2.09±0.17 hi	2.29±0.19 efg	2.23±0.09 fg	1.79±0.25 klm
蔗糖	2.39±0.10 cde	2.59±0.13 a	2.42±0.13 bcde	2.39±0.40 cde
低聚木糖	2.29±0.12 efg	2.35±0.13 def	1.74±0.04 lmn	2.05±0.07 i
淀粉	2.41±0.08 bcde	2.52±0.14 abc	2.54±0.14 ab	2.22±0.25 fg
纤维素	2.59±0.12 a	2.40±0.18 cde	2.47±0.09 abcd	2.57±0.22 a
木聚糖	1.98±0.17 ij	1.86±0.06 jkl	1.75±0.06 lmn
木质素	2.18±0.17 gh	1.69±0.11 mn
CK	2.34±0.10 def
碳源	W192
碳源	0.50%	1%	2%	4%
葡萄糖	2.89±0.10 ijk	3.09±0.06 def	3.00±0.09 gh	2.07±0.05 s
木糖	3.07±0.14 efg	2.90±0.03 ij	2.58±0.05 op	1.76±0.05 t
果糖	2.58±0.06 op	2.59±0.07 op	2.73±0.11 mn	2.16±0.15 r
海藻糖	3.09±0.05 def	3.18±0.08 bc	3.17±0.06 bcd	3.23±0.04 ab
纤维二糖	2.81±0.10 klm	2.74±0.10 mn	2.32±0.08 q	1.54±0.07 u
麦芽糖	3.09±0.10 def	3.19±0.08 bc	3.06±0.11 efg	2.95±0.08 hi
蔗糖	3.17±0.11 bcd	3.11±0.10 cdef	3.10±0.06 cdef	3.29±0.07 a
低聚木糖	2.97±0.14 hi	3.12±0.04 cde	2.83±0.07 jkl	2.54±0.11 p
淀粉	3.14±0.13 cde	3.03±0.07 fgh	2.91±0.10 ij	2.78±0.12 lm
纤维素	3.11±0.08 cdef	3.16±0.06 bcd	2.97±0.16 hi	2.86±0.11 jkl
木聚糖	2.66±0.09 no	2.66±0.08 no	2.13±0.08 rs
木质素	2.66±0.12 no	2.34±0.18 q
CK	3.10±0.04 cdef

碳源	A15
碳源	0.50%	1%	2%	4%
葡萄糖	0.14±0.01hij	0.15±0.01ghij	0.17±0.01ghij	0.17±0.02ghij
木糖	0.13±0.01hij	0.12±0.01hij	0.22±0.03ghij	0.22±0.02ghij
果糖	0.18±0.02ghij	0.16±0.02ghij	0.18±0.00ghij	0.18±0.01ghij
海藻糖	0.22±0.02ghij	0.23±0.03ghij	0.23±0.02ghij	0.27±0.01ghij
纤维二糖	0.15±0.00ghij	0.17±0.01ghij	0.15±0.00ghij	/
麦芽糖	0.20±0.06ghij	0.20±0.03ghij	0.21±0.01ghij	0.26±0.02ghij
蔗糖	0.18±0.02ghij	0.18±0.02ghij	0.21±0.04ghij	0.30±0.08ghi
低聚木糖	0.22±0.05ghij	0.24±0.01ghij	0.39±0.08fg	0.20±0.03ghij
淀粉	0.18±0.02ghij	0.33±0.03gh	0.71±0.10e	1.80±0.20c
纤维素	0.56±0.05ef	1.11±0.14d	2.05±0.25b	3.86±0.70a
木聚糖	0.13±0.01hij	0.15±0.01hij	/	/
木质素	0.06±0.02ij	0.04±0.01ij	/	/
CK	0.13±0.01hij
碳源	W192
碳源	0.50%	1%	2%	4%
葡萄糖	0.24±0.03fghij	0.23±0.06fghij	0.26±0.01fghij	0.26±0.01fghij
木糖	0.25±0.01fghij	0.21±0.05fghij	0.20±0.01fghij	0.19±0.02ghij
果糖	0.24±0.01fghij	0.28±0.03fghij	0.30±0.01fghi	0.29±0.02fghi
海藻糖	0.36±0.01fgh	0.35±0.01fgh	0.41±0.04fg	0.36±0.05fgh
纤维二糖	0.15±0.02hij	0.15±0.03hij	0.16±0.04hij	/
麦芽糖	0.30±0.06fghi	0.33±0.01fgh	0.34±0.04fgh	0.40±0.06fg
蔗糖	0.33±0.04fgh	0.33±0.10fgh	0.43±0.06ef	0.37±0.02fgh
低聚木糖	0.23±0.00fghij	0.32±0.02fghi	0.27±0.05fghij	0.21±0.08fghij
淀粉	0.28±0.02fghij	0.40±0.06fg	0.69±0.03d	0.96±0.05c
纤维素	0.62±0.08de	1.04±0.29c	2.03±0.36b	3.92±0.57a
木聚糖	0.16±0.09hij	0.18±0.03ghij	/	/
木质素	0.05±0.02j	0.09±0.02ij	/	/
CK	0.19±0.01ghij

碳源	A15
碳源	0.50%	1%	2%	4%
葡萄糖	0.14±0.01hij	0.15±0.01ghij	0.17±0.01ghij	0.17±0.02ghij
木糖	0.13±0.01hij	0.12±0.01hij	0.22±0.03ghij	0.22±0.02ghij
果糖	0.18±0.02ghij	0.16±0.02ghij	0.18±0.00ghij	0.18±0.01ghij
海藻糖	0.22±0.02ghij	0.23±0.03ghij	0.23±0.02ghij	0.27±0.01ghij
纤维二糖	0.15±0.00ghij	0.17±0.01ghij	0.15±0.00ghij	/
麦芽糖	0.20±0.06ghij	0.20±0.03ghij	0.21±0.01ghij	0.26±0.02ghij
蔗糖	0.18±0.02ghij	0.18±0.02ghij	0.21±0.04ghij	0.30±0.08ghi
低聚木糖	0.22±0.05ghij	0.24±0.01ghij	0.39±0.08fg	0.20±0.03ghij
淀粉	0.18±0.02ghij	0.33±0.03gh	0.71±0.10e	1.80±0.20c
纤维素	0.56±0.05ef	1.11±0.14d	2.05±0.25b	3.86±0.70a
木聚糖	0.13±0.01hij	0.15±0.01hij	/	/
木质素	0.06±0.02ij	0.04±0.01ij	/	/
CK	0.13±0.01hij
碳源	W192
碳源	0.50%	1%	2%	4%
葡萄糖	0.24±0.03fghij	0.23±0.06fghij	0.26±0.01fghij	0.26±0.01fghij
木糖	0.25±0.01fghij	0.21±0.05fghij	0.20±0.01fghij	0.19±0.02ghij
果糖	0.24±0.01fghij	0.28±0.03fghij	0.30±0.01fghi	0.29±0.02fghi
海藻糖	0.36±0.01fgh	0.35±0.01fgh	0.41±0.04fg	0.36±0.05fgh
纤维二糖	0.15±0.02hij	0.15±0.03hij	0.16±0.04hij	/
麦芽糖	0.30±0.06fghi	0.33±0.01fgh	0.34±0.04fgh	0.40±0.06fg
蔗糖	0.33±0.04fgh	0.33±0.10fgh	0.43±0.06ef	0.37±0.02fgh
低聚木糖	0.23±0.00fghij	0.32±0.02fghi	0.27±0.05fghij	0.21±0.08fghij
淀粉	0.28±0.02fghij	0.40±0.06fg	0.69±0.03d	0.96±0.05c
纤维素	0.62±0.08de	1.04±0.29c	2.03±0.36b	3.92±0.57a
木聚糖	0.16±0.09hij	0.18±0.03ghij	/	/
木质素	0.05±0.02j	0.09±0.02ij	/	/
CK	0.19±0.01ghij

碳源	漆酶/ [nmol/(min·mL)]			MnP/ [nmol/(min·mL)]		ACX/ [nmol/(min·mL)]		β-木糖苷酶/ [nmol/(min·mL)]		滤纸酶 /[mg/(min·mL)]		C1/ [μg/(min·mL)]	CX/ [μg/(min·mL)]	β-GC/ [nmol/(min·mL)]
葡萄糖	2858.38±567.01def			0.40±0.05c		1309.35±219.22b		8.61±0.65e		1.06±0.06d		329.82±64.10a	25.34±1.39b	2.18±0.43cd
木糖	3545.96±241.91abc			0.44±0.05c		1573.54±281.66a		7.52±1.48e		1.18±0.11d		155.12±27.57d	25.69±4.76b	2.61±0.10abc
果糖	3175.38±356.27bcde			2.04±0.46b		1091.56±81.87bc		12.40±1.25d		0.92±0.16d		213.43±24.28c	26.07±1.45b	2.42±0.12bcd
海藻糖	2792.57±118.19ef			0.82±0.03c		531.28±85.64efg		16.11±2.51c		1.63±0.02c		133.45±4.13d	10.38±0.24d	2.12±0.06cd
纤维二糖	3699.31±307.02a			0.48±0.02c		378.16±51.93fg		12.51±0.81d		0.91±0.07d		157.98±16.49d	17.18±1.51c	2.40±0.10bcd
麦芽糖		3078.07±250.72cde	0.89±0.15c		291.16±57.43g		15.48±2.30c		3.78±0.38a		265.50±27.38b		59.86±8.29a	2.09±0.07d
蔗糖		3348.14±49.42abcd	0.91±0.11c		315.52±57.56fg		15.73±1.57c		1.13±0.18d		140.96±5.46d		10.42±0.13d	2.07±0.17d
低聚木糖		3621.54±169.62ab	4.85±0.96a		711.08±114.29de		21.15±1.89b		3.99±0.19a		282.21±20.19b		26.21±2.85b	2.21±0.14cd
淀粉		3122.43±236.07cde	0.75±0.06c		557.96±106.26ef		16.24±1.96c		2.17±0.23b		171.08±5.35d		10.95±0.31d	2.09±0.04d
纤维素		3056.67±266.37cde	0.96±0.16c		430.94±76.25fg		17.12±1.70c		0.94±0.19d		125.89±2.29d		10.30±0.15d	2.56±0.28abcd
木聚糖		2064.05±100.61g	0.84±0.16c		1046.32±190.72c		15.26±0.83cd		0.99±0.12d		219.50±23.84c		12.94±2.36cd	2.54±0.19abcd
木质素		647.89±56.68h	1.91±0.12b		809.10±155.23d		30.88±2.18a		1.17±0.05d		161.93±19.66d		13.24±1.68cd	2.72±0.49ab
CK		2449.68±206.27fg	0.76±0.10c			328.86±68.35fg	21.90±0.38b		1.09±0.17d		127.84±1.78d		10.16±0.30d	3.02±0.52a