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• 2025 Vol.41 No.9 Published:25 March 2025

  Effect of Graphene Oxide on Rice Seed Germination Under Salt Stress LIANG Wenzhao, ZHOU Shiyi, WEI Ruiyan, SHI Linya, LIU Naixin, YU Qingtao 2025, 41 (9):  1-7.  DOI: 10.11924/j.issn.1000-6850.casb2024-0491 Abstract + ( 87 )   HTML ( 4 )   PDF ( 1332KB )   ( 10 ) To explore the effect of graphene oxide (GO) treatment on rice seed germination under salt stress, this experiment used the rice variety 'Harbin Japonica Rice 15' as the experimental material. Under normal conditions and salt stress (0.05 mol/L NaCl), GO was induced at different mass concentrations (0, 25, 50, 100 mg/L) to detect the changes in antioxidant enzyme activity, malondialdehyde, and osmotic regulator content of rice seeds after different treatments. The results show that: (1) low concentrations of GO (25 mg/L) can promote the germination of rice seeds, while high concentrations of GO (50, 100 mg/L) significantly inhibit their germination. 25 mg/L GO can increase seed vitality and improve germination rate under salt stress; (2) high concentrations of GO and NaCl combined treatment can further exacerbate the inhibitory effect of salt stress on rice seed growth, increase antioxidant enzyme activity, reduce malondialdehyde content, and alleviate oxidative stress response; (3) GO treatment promotes the synthesis and accumulation of osmotic regulators, increases the osmotic regulation ability and salt resistance of rice seeds, strengthens the salt tolerance of seedlings, and significantly improves their emergence and seedling efficiency in salt environments. Low concentration GO can promote rice germination, while high concentration GO has certain harm to rice growth. Especially under salt stress, high concentrations of GO can increase the toxicity of salt stress on rice growth. It is suggested that high concentration GO related fertilizers should be used with caution in rice production practice in saline alkali areas.

  Effects of Different Exogenous Silicon Fertilizers Application on Yield, Dry Matter and Nutrient Accumulation and Transport of Rice in Tidal Flat CHEN Pengjun, ZHANG Jiao, HAN Jijun, MIAO Yuanqing, CUI Shiyou 2025, 41 (9):  8-17.  DOI: 10.11924/j.issn.1000-6850.casb2024-0619 Abstract + ( 60 )   HTML ( 4 )   PDF ( 1257KB )   ( 12 ) A field experiment was carried out from June to November 2021 in tidal flat of Nantong, Jiangsu Province to study the effects of different exogenous silicon fertilizers spraying on rice yield, dry matter and nutrient accumulation and transport in various organs and rice quality, with ‘Nanjing 5055’ as the test variety. Five treatments were set up in the experiment, including spraying water treatment (CK), sugar alcohol silicon treatment (SF1), seaweed liquid silicon treatment (SF2), liquid silica-zinc fertilizer treatment (SF3) and highly active ionic liquid silicon treatment (SF4). The results showed that (1) compared with CK, rice yield under SF1, SF2, SF3 and SF4 increased by 9.24%, 7.31%, 0.28% and 3.64%, respectively, and reached a significant level under SF1. At the same time, the number of grains per panicle, the number of solid grains, the weight of thousand grains, the length of panicle and the density of grains were increased significantly under SF1 (P<0.05). (2) The above ground dry matter accumulation of rice at maturity was SF1>SF2>SF4>SF3>CK. At heading stage, nitrogen accumulation in the above-ground part and potassium accumulation in the upper part of the ground under SF1 and SF3 were significantly increased under each spraying treatment. Nitrogen accumulation in the lower panicle and above-ground parts of SF1, SF3 and SF4 at maturity was significantly increased (P<0.05). The distribution of nitrogen and potassium accumulation in each organ of rice at heading stage was in the order of stem sheath>leaf>ear. The distribution of nitrogen accumulation at maturity was in the order of spike>sheath>leaf, and the distribution of potassium accumulation was in the order of sheath>ear>leaf. (3) Compared with CK, leaf dry matter transfer (SF3) and ear dry matter increase (SF1, SF2 and SF3) were significantly increased. Nitrogen transfer in stem sheath (SF2, SF3 and SF4) and leaves (SF1) and nitrogen increase in panicle (SF1, SF3 and SF4) were significantly increased. The transfer volume of potassium in stem sheath (SF3) and leaf (SF1) and the increase of potassium in panicle (SF3) were significantly increased (P<0.05). (4) Rice yield was positively correlated with spike dry matter increase and nitrogen transfer, significantly positively correlated with dry matter accumulation and spike nitrogen accumulation at maturity, and significantly negatively correlated with leaf potassium accumulation. (5) Different exogenous silicon fertilizers spraying had no significant effects on the appearance and processing quality of shoal rice under the experimental conditions. Therefore, spraying appropriate exogenous silicon fertilizer (especially sugar alcohol silicon) on the leaf surface could significantly increase rice yield in this coastal mudflat area. Spraying exogenous silicon fertilizer mainly improves the number of grains per spike and the quality of 1000 grains, increasing the accumulation of dry matter and nitrogen in the rice spike.

  Effects of Microbial Product Coupling Urea on Straw Decomposition Rate and Yield of Rice LI Yangyang, CHEN Shuaimin, XU Minghong, CHI Chang, MA Wei, WANG Yinping, SONG Yan, FAN Zuowei, WU Haiyan 2025, 41 (9):  18-24.  DOI: 10.11924/j.issn.1000-6850.casb2024-0317 Abstract + ( 56 )   HTML ( 4 )   PDF ( 1150KB )   ( 6 ) To select the most effective microbial product for in-situ decomposition of rice straw under cool conditions in Northeast China, and to determine the optimal dosage of microbial product coupling urea, this study conducted comparative experiments, and used the decomposition rate of rice straw, biological characteristics and yield of rice as indicators to define the optimal microbial product and the dosage of urea. The results showed that the independently developed microbial product NKY showed better field application effects. The straw decomposition rates of Hongqi Farm and Wanchang Town were 68.8% and 67.2%, respectively, which were 38.5% and 35.5% higher than CK. The height, tiller number, and dry weight of rice were also increased, and the rice yield increased by 6.0% and 6.8%, respectively. The optimal dosage for coupling urea with microbial product NKY was 75 kg/hm2. Compared with no urea application, the straw decomposition rate increased by 8.7%, and the yield increased by 6.2%. In summary, the microbial product NKY not only has good degradation ability of rice straw, but also could promote crop growth and improve crop yield, which has a better application prospect.

  Effect of Spraying Alginate Enhancing Solution at Different Growth Stages on Photosynthetic Characteristics and Yield of Winter Wheat TIAN Cuiling, TIAN Jialiang 2025, 41 (9):  25-31.  DOI: 10.11924/j.issn.1000-6850.casb2024-0778 Abstract + ( 53 )   HTML ( 2 )   PDF ( 1202KB )   ( 7 ) To explore the effect of alginate enhancing solution on the photosynthetic characteristics and yield of winter wheat, four different concentration treatments were set during the regreening stage, jointing stage and booting stage of winter wheat, which were 0 mg/kg (CK), 30 mg/kg (N30), 60 mg/kg (N60) and 90 mg/kg (N90). The indexes such as chlorophyll content, grain filling rate, dry matter accumulation and distribution, photosynthetic characteristics and yield among different treatments were studied. The results showed that spraying alginate enhancing solution could significantly increase the chlorophyll content of wheat flag leaves and the grain filling rate, especially in the late stage of flowering, it still maintained a significant advantage compared with CK. The dry matter accumulation of stem sheath + leaves, ear shaft + glume, grain and distribution ratio were significantly higher than those of CK, among which the advantage of N30 treatment at the jointing stage was the most obvious. In photosynthesis, spraying alginate enhancing solution significantly increased the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of flag leaves, reduced the intercellular carbon dioxide concentration (Ci) of flag leaves, keeping the crops at a high photosynthetic rate all the time. In terms of yield and composition factors, there were no significant differences in the number of spikes and grains per spike among different concentration treatments compared with CK, but the thousand grain weight and yield were significantly higher than CK. Among them, spraying alginate enhancing solution at 30 mg/kg at the jointing stage had the best yield-increasing effect, with a yield of 8811.96 kg/hm2, which was 12.01% higher than that of the CK treatment in the same growth period. In summary, spraying alginate enhancing solution at 30 mg/kg at the jointing stage is a more reasonable fertilization scheme.

  Study of Yield and Quality Traits of New Wheat Cultivar ‘Hemai 26’ REN Qingguo, WU Guangjun, LIN Ping, ZHANG Jiyu, ZHANG Xin, ZHANG Yongshan, HAI Tao 2025, 41 (9):  32-37.  DOI: 10.11924/j.issn.1000-6850.casb2024-0405 Abstract + ( 53 )   HTML ( 2 )   PDF ( 1152KB )   ( 8 ) To further understand the production characteristics and applicable value of the wheat variety ‘Hemai 26’, its yielding ability, yielding stability and adaptability as well as resistibility and quality characteristic were analyzed with ‘Zhoumai 18’ as the contrast using the regional tests of wheat varieties in southern Huang-Huai Wheat Region consisted of institutions and enterprises during 2016-2018 and production tests in 2018-2019. The results showed that the yield of ‘Hemai 26’ increased by 4.5%, 11.53% and 5.28% respectively compared with that of ‘Zhoumai 18’ during 2016-2019, and reached a significant level. Coefficient of variation (CV) of ‘Hemai 26’ was slightly smaller, the high stability coefficient (HSC) was larger and adaptability was higher than that of ‘Zhoumai 18’. The average yield of each experimental variety showed linear regression with the average yield of ‘Hemai 26’ in different test sites, and the regression coefficient tended to 1. ‘Hemai 26’ has strong lodging resistance and general disease resistance. ‘Hemai 26’ has good grain uniformity, high grain bulk density and good quality. ‘Hemai 26’ has outstanding yielding ability, good yielding stability, wide adaptability and excellent quality, which has good promotion and utilization value in Southern Huang-Huai Wheat Region.

  Effects of Abscisic Acid on Growth and Cold Resistance of Phaseolus vulgaris Seedlings Under Low Temperature Stress JIANG Zijian, YANG Maolin, YANG Xiaoxu, LIU Chang, LIU Dajun, FENG Guojun 2025, 41 (9):  38-46.  DOI: 10.11924/j.issn.1000-6850.casb2024-0437 Abstract + ( 51 )   HTML ( 4 )   PDF ( 1356KB )   ( 12 ) The objective of this study is to investigate the impact of abscisic acid (ABA) on the growth and cold resistance of Phaseolus vulgaris seedlings under low temperature stress, with a focus on elucidating the regulatory mechanism by which ABA mitigates cold damage in beans. Seedlings of low-temperature sensitive Phaseolus vulgaris variety ‘Genoa’ were used in this study as experimental materials. The experimental groups consisted of six categories: the control group maintained at normal temperature (CK), the group subjected to low temperature treatment (LT), and ABA solution groups with concentrations of 10 mg/L (T10), 20 mg/L (T20), 30 mg/L (T30), and 40 mg/L (T40) respectively. The seedlings in the ABA solution treated group were then subjected to low temperature stress. The growth and physiological indexes of the 6 groups of Phaseolus vulgaris seedlings were measured. The results demonstrated that under T10 treatment, the growth index of bean seedlings reached its peak. Furthermore, compared to the LT group, plant height, root length, stem diameter, and leaf area in the T10 group were increased significantly by 48.47%, 43.46%, 73.20%, and 65.69% respectively. Additionally, ABA spray reduced electrolyte permeability in bean seedlings under low temperature stress while enhancing the activities of antioxidant enzymes POD and SOD. This led to clearance of excess reactive oxygen species (ROS) as well as reductions in malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-), and soluble protein content. Moreover, ABA application protected the cell membrane structure of bean seedlings while improving their photosynthetic performance under low temperature stress. In conclusion, an appropriate concentration of ABA can promote the growth and development of bean seedlings under conditions of low temperature stress.

  Transcriptome Analysis in Roots of Sweetpotato with Different Drought Tolerance WANG Baoqing, XIE Beitao, ZHANG Liming 2025, 41 (9):  47-55.  DOI: 10.11924/j.issn.1000-6850.casb2024-0439 Abstract + ( 53 )   HTML ( 2 )   PDF ( 1543KB )   ( 5 ) In order to reveal the difference between thick root (TR) and storage root (SR) of sweetpotato with difference drought tolerance from transcriptional level, and further explicit the mechanism of roots differentiation and drought tolerance by simulating field drought in dry shed with artificial water control, two varieties including ‘Jishu21’ (‘JS21’ ) with strong drought tolerance and ‘Jizishu1’ (‘JZ1’) with weak drought tolerance were chosen as research materials. Transcriptome analysis of sweet potato thick root and storage root was carried out. The results indicated GO classification of differentially expressed genes (DEGs) of the two varieties was similar to that of KEGG enrichment. Under drought stress, 84 DEGs between SR and TR of ‘JS21’ mainly enriched the accumulation of starch and sucrose metabolic pathway, while 112 DEGs between SR and TR of ‘JZ1’ mainly enriched ribosomal metabolic pathway. Among different varieties, 224 DEGs between ‘JS21’ and ‘JZ1’ were found in SR, but 204 DEGs between ‘JS21’ and ‘JZ1’ were counted in TR, and all mainly enriched ribosomal metabolic pathway. Further analysis by transcription factors and hormones metabolism showed that ARF-like transcription factors in storage root and NAC-like transcription factors in thick root of ‘JS21’ were more differentially expressed than ‘JZ1’ under drought stress. The expression of genes involved in carotenoid biosynthesis pathway was up-regulated, while genes involved in brassinosteroid biosynthesis pathway were down-regulated in both thick root and storage root of ‘JS21’. In the same variety, the gene expression of α-linolenic acid metabolic pathway in ‘JS21’ storage root was up-regulated compared with thick root. In conclusion, sweetpotato with strong drought tolerance can maintain starch and sucrose metabolism under drought conditions by coordinating the expression of drought tolerance genes and growth-related transcription factors, balancing drought tolerance hormones (ABA, JA) and growth related hormones (IAA, BR), so as to improve the drought resistance and ensure the formation of yield.

  Evaluation of Drought Tolerance of Introduced Blueberry Varieties in Northwest Yunnan SU Zechun, YANG Wenhong, YANG Yanlin, YANG Zhengsong, HE Jianping, MU Yongqing, YU Ying, HE Jiawei 2025, 41 (9):  56-64.  DOI: 10.11924/j.issn.1000-6850.casb2024-0517 Abstract + ( 68 )   HTML ( 2 )   PDF ( 1284KB )   ( 14 ) In order to solve the problem of water shortage faced by blueberry planting in high-altitude mountainous areas, this study takes 11 introduced blueberry varieties as experimental materials. It aims to evaluate the drought tolerance potential of the varieties under natural drought conditions by setting up the comparative experiment method of the drought group and the watering group. In 2021, leaf morphological and anatomical observations were carried out on blueberries in the drought group. Statistical methods were used to explore the drought tolerance of different blueberry varieties while screening key indicators for leaf morphology and anatomy. In 2022, a bivariate analysis of variance method was employed to compare the yield components between the drought group and the watered group, in order to assess the production recovery potential of each variety after being affected by drought. The results showed that there were significant differences among different blueberry varieties in terms of drought tolerance. The order of drought tolerance of the 11 blueberry varieties from strong to weak was as follows: ‘Misty’> ‘Bluecrop’> ‘Sweetheart’> ‘Brigitta’> ‘Jubilee’> ‘Bluecrop’> ‘Shengshi’> ‘O'Neal’> ‘Boniface’> ‘Garden blue’> ‘Duke’. Through screening 10 leaves morphological and anatomical indicators, 4 typical indicators for evaluating the drought tolerance of blueberries were obtained, namely, the thickness of the upper epidermis, the thickness of the leaf, the thickness of the spongy tissue and the stomatal density. The results of the empirical analysis showed that ‘Misty’, ‘Bluecrop’ and ‘Sweetheart’ had relatively strong drought tolerance, while ‘Boniface’, ‘Garden blue’ and ‘Duke’ had relatively weak drought tolerance. This conclusion was consistent with that of the drought tolerance evaluation.

  Influence of LED Light Quality on Growth and Quality of Tissue Culture Plantets of Dendrobium officinale and D. nobile JIANG Yongqiang, WU Yao, FANG Zhongjian, YE Guangying, HUI Jun’ai, ZHOU Rong, WANG Zaihua 2025, 41 (9):  65-72.  DOI: 10.11924/j.issn.1000-6850.casb2024-0365 Abstract + ( 54 )   HTML ( 1 )   PDF ( 1457KB )   ( 12 ) To improve the efficiency and quality level of medicinal Dendrobium tissue culture, this study used D. officinale and D. nobile as test materials to investigate the effects of eight different LED light quality combinations, including red, blue, green, and white light (R6B3G0, R6B3G1, R6B3G3, R3B6G0, R3B6G1, R3B6G3, R3B3G3, W), on the proliferation, rooting, and active component contents of the kinds of Dendrobium. The results showed that both D. officinale and D. nobile had the best proliferation effects under the light quality culture of R3B6G3, with proliferation coefficients of 6.67 and 1.59, respectively, which were significantly increased by 72.8% and 101.3% compared to the control (W), with D. nobile showing the highest fresh weight under this treatment. Under the treatment of R6B3G3, the best rooting effects were observed for both D. officinale and D. nobile, with individual root numbers of 10.56 and 9.42 per plant, respectively, which were increased by 59.5% and 75.4% compared to W. At the same time, it showed the highest fresh and dry weight contents of D. officinale under this treatment, with 1.254 and 0.135 g/plant, respectively, significantly increasing by 81.7% and 1.08 times compared to W. The dry matter content was highest under the R3B6G1 treatment, significantly increasing by 29.0% compared to W, and the polysaccharide content reached its maximum value of 24.61% under this treatment, while the total flavonoid content was highest under W, at 1.46%. Under the treatment of R6B3G0 and R3B3G3, the content of total phenolics was highest of D. officinale, both increasing by 1.39 times compared to W, and the dendrobine contents of D. nobile was optimal, both at 0.5%. In short, it was beneficial for the proliferation of D. officinale adding stronger green light under the treatment of R3B6, while it was beneficial for the proliferation of D. nobile adding stronger green light under R3B6 or weaker green light under R6B3. In addition, it was effective for rooting of two Dendrobium varieties adding weaker green light under the treatment of R6B3. Furthermore, it was conducive to the accumulation of dry matter and polysaccharide in D. officinale adding weaker green light under the treatment of R3B6, while it was beneficial for the accumulation of dry matter and dendrobine in D. nobile with stronger green light under the treatment of R6B3.

  Effect of Exogenous Inositol on Yield and Quality of Tomatoes Under Salt Stress HUANG Wenyin, ZHANG Baige, CHANG Jingjing, CHEN Xiao, LI Jing, CHEN Lei, ZHAO Junhong, LUO Mouxiong, SONG Zhao 2025, 41 (9):  73-80.  DOI: 10.11924/j.issn.1000-6850.casb2024-0489 Abstract + ( 66 )   HTML ( 3 )   PDF ( 2207KB )   ( 5 ) The paper aims to investigate the optimal concentration of exogenous inositol and its effect on yield, quality and antioxidant system of tomato under salt stress, and to provide some theoretical basis for the application of inositol in quality improvement of tomato production. In the present study, five inositol concentration gradients of 0% (CK), 0.05% (MI1), 0.1% (MI2), 0.2% (MI3) and 0.4% (MI4) were established to investigate the effects of exogenous inositol on tomato yield and quality using ‘Hongjian’ cherry tomato as the test material. On this basis, the 100 mmol/L NaCl salt stress treatment (N) and the inositol treatment (NM3) with 0.2% inositol were established to investigate the role of inositol in alleviating salt stress in tomato. With increasing inositol concentration, tomato yield showed an overall trend of increasing and then decreasing, in which the tomato yield of MI3 treatment was significantly increased by 17.7% compared to that of CK. The increase in tomato yield by exogenous inositol application was closely related to the increase in leaf SPAD value, the promotion of root growth and the enhancement of the activity of key antioxidant enzymes (e.g. POD activity). Low concentrations of inositol (MI1 and MI2) significantly increased the vitamin C content of the fruits, while increasing the concentration of inositol had no significant effect on the vitamin C content of the fruits. The soluble sugar content of the fruits significantly increased by 38.6%-166.2%, while the soluble protein content decreased by 13.4%-22.6% after the application of exogenous inositol. Salt stress significantly reduced tomato yield, leaf SPAD and vitamin C content by 16.1%, 14.4% and 26.4% respectively compared to CK by inhibiting root growth, increasing malondialdehyde (MDA) content and decreasing enzyme activities of the antioxidant system. On the contrary, application of inositol (NM3) under salt stress significantly promoted root growth, reduced MDA content and increased the activity of antioxidant system enzymes, resulting in a significant increase in tomato yield, leaf SPAD, vitamin C and lycopene content by 37.8%, 13.5%, 23.8% and 34.5% compared with N treatment. The application of exogenous inositol at an appropriate concentration (MI3) could significantly promote tomato root growth and increase the activities of key antioxidant enzymes, thus increasing tomato yield and improving fruit quality; furthermore, the application of exogenous inositol at an appropriate concentration could significantly alleviate salt stress in tomato and improve tomato yield and quality.

  Research on Heavy Metal Sources and Environmental Evaluation of Soil in Yuanmou, Central Yunnan XU Jie, ZHANG Ya, LI Pingzhao, XU Lei, CHENG Yanxun, WEN Fangping 2025, 41 (9):  81-90.  DOI: 10.11924/j.issn.1000-6850.casb2024-0623 Abstract + ( 51 )   HTML ( 2 )   PDF ( 2574KB )   ( 4 ) By identifying the distribution characteristics of heavy metal elements in Yuanmou County, we can understand the local heavy metal sources and environmental ratings, objectively grasp the environmental problems existing in the soil in this area, put forward scientific and reasonable suggestions for agricultural development and environmental governance, and improve the level of land management and environmental monitoring in this area. The method of combining traditional geochemistry and soil science was used, and the sampling was carried out according to the relevant standards of 1:250000 land quality geochemical survey. SPSS, Excel, GeolPAS.V4.5, ArcGIS10.8 and other software were used for data modeling, result integration and map production. The results showed that heavy metal elements As, Cd, Cr, Cu, Hg, Ni, Pb and Zn were existed in the surface soil of Yuanmou County, in which Cd was enriched on the surface, and the anisotropy of Cd and Hg was higher than that of other elements. The overall distribution of Cu was balanced, with local characteristics of enrichment and depletion. Cr was highly correlated with Ni and weakly negatively correlated with Pb. There were three main sources of heavy metals: mainly rich in Zn and As, mainly rich in Cr, Ni and Cu, and mainly rich in Hg. In the comprehensive grade assessment of surface soil environment, the risk-free area was 1873 km2, accounting for 92.77%, and the risk-controlled area was 146 km2, accounting for 7.23%. The area was concentrated in Jiangyi Town, Guanyuan Town and Pingtian Town, and scattered in other areas, with no high risk area. The distribution of heavy metals As, Cr, Ni, Pb and Zn in Yuanmou County was mainly controlled by the parent material, Cu and Cd were controlled by the parent material, continuous weathering and human activities, and Hg was mainly controlled by human activities. There was no high-risk area in Environmental rating, and the risk controllable areas were mainly affected by Cu and Cd, among which the Cd risk controllable areas were highly consistent with the concentrated areas of agricultural development, and the application of relevant pesticides should be reasonably controlled and monitored in the later stage. Hg wasn’t at risk in the whole region, but there had been a slight enrichment trend in the surface soil, which required a late warning to avoid pollution.

  Effect of Organic Amendment Combined with Moderate Deep Ploughing on Productivity Improvement of Hilly Reclaimed Rice Field WANG Feng, ZHU Shijun, YING Hong, CHAI Weigang, DAI Yaolu, YUAN Qing, JIN Shuquan 2025, 41 (9):  91-98.  DOI: 10.11924/j.issn.1000-6850.casb2024-0605 Abstract + ( 54 )   HTML ( 2 )   PDF ( 1104KB )   ( 7 ) To solve the practical problems in the existing “non-grain” cultivated land improvement technology, such as high cost and poor regional suitability, a field experiment was carried out in the typical “non-grain” reclaimed rice field in the hilly area of eastern Zhejiang Province. The effects of different organic amendments and combinations (T1: biochar based fertilizer, T2: potassium fulvic acid, T3: organic fertilizer + biochar based fertilizer, T4: organic fertilizer + potassium fulvic acid), and tillage depth (D: deep tillage with 20 cm, S: shallow tillage with 15 cm) on topsoil properties, rice yield and economic benefits were analyzed. The results showed that the application of different organic amendments could significantly increase the yield of rice, among which T4 and T3 had the best effects, especially under the condition of deep ploughing. Compared with the conventional control (S-CK), they could increase the yield by 3.7 t/hm2 (48.8%) and 3.4 t/hm2 (44.5%) respectively, and the economic benefit reached 2391-3101 yuan/hm2 in the current season. Deep ploughing decreased soil pH by 0.2 units on average, but significantly increased soil organic matter (SOM), total nitrogen (TN), alkali-hydro nitrogen (AN), available phosphorus (AP) and cation exchange capacity (CEC). Among them, the SOM in D-T4 and D-T3 increased by 11.5% and 11.3%, respectively, compared with S-CK. The correlation analysis showed that rice yield was significantly positively correlated with agronomic characters (effective panicle, panicle length, spikelet per panicle and seed setting rate) and soil fertility indexes (SOM, TN, AN, AP, AK and CEC) (P<0.01). Therefore, the organic amendments combinations of organic fertilizer and biochar based fertilizer or potassium fulvic acid, with moderate mechanical deep ploughing, can simultaneously achieve tillage layer reconstruction and directional fertilizer cultivation, and promote soil improvement efficiency in reclamation area. This technology has great potential to be popularized in the “non-grain” reclaimed rice field in hilly areas.

  Spectral Characteristics of Soil Water-soluble Organic Carbon Affected by Cultivation Methods During Rice Growth Period DONG Linlin, YAN Kai, SHENG Xuewen, LU Changying, SHI Linlin, WANG Haihou, WU Zhenggui 2025, 41 (9):  99-106.  DOI: 10.11924/j.issn.1000-6850.casb2024-0624 Abstract + ( 50 )   HTML ( 2 )   PDF ( 1130KB )   ( 6 ) Soil water-soluble organic carbon (WSOC) changed with cultivation methods is of great significance to crop growth and soil carbon cycle. In this study, a field experiment was conducted to compare the effects of rotary tillage (RT) and no tillage (NT) on the content and spectral characteristics of WSOC during the key growth period of rice in rice-wheat rotation system in the Taihu Lake Region. The results showed that the content of soil organic carbon (SOC) in RT and NT treatments increased from 21.73 g/kg and 21.30 g/kg before rice planting to 21.95 g/kg and 23.49 g/kg after rice harvest, respectively. Meanwhile, the content of water-soluble organic carbon in RT and NT treatments increased from 1.74 g/kg and 1.66 g/kg before rice planting to 3.74 g/kg and 4.35 g/kg after rice harvest, respectively. No tillage for rice-wheat rotation system was more conducive to increasing soil carbon sequestration and water-soluble organic carbon. Under the both tillage methods, the SUVA254 and SUVA260 of WSOC after rice harvest decreased slightly compared to that before planting rice, and the SUVA254 and SUVA260 of NT were higher than those of RT, while E2/E3 and E3/E4 were both increased. No tillage was more conducive to improving the aromaticity and humification of WSOC than RT for rice-wheat rotation system. Compared to rotary tillage, no tillage was more conducive to improving the stability of WSOC and had a positive effect on increasing soil carbon sequestration in paddy soil of rice-wheat rotation system.

  Forms and Spatial Distribution of Soil Phosphorus in Subtropical Natural Forest Land ZHOU Huaping, ZHANG Zhouna, ZHANG Mingkui 2025, 41 (9):  107-116.  DOI: 10.11924/j.issn.1000-6850.casb2024-0588 Abstract + ( 51 )   HTML ( 2 )   PDF ( 1174KB )   ( 5 ) To understand the spatial differentiation of soil P forms in the subtropical natural forest land, this study took the natural forest land of Lianhuashan scenic spot in the Qianjiangyuan National Park as the research object, both upper layer samples (0-15 cm and 15-30 cm) and the horizontal samples of representative soil profiles located in three terrain positions, including the top of slope, middle slope (hillside), slope toe, and also rhizosphere soils at the foot of the slope, were collected to characterize P forms for understanding the relationship between soil P composition and topography. The analysis results of both upper soil sample and profile soil horizonal samples showed that the organic C, total P, total organic P and various forms of organic P, Ca2-P and available P had obvious surface enrichment characteristics, which gradually decreased with the soil depth. The averaged proportion of inorganic P in the total P was higher than that of organic P. The proportion of organic P to the total P and the proportions of both active and moderate active organic P to total organic P decreased in the order of slope toe > top > middle slope, and also decreased with the depth of the profile. The organic P in forest soil mainly existed in the medium active organic P and medium stable organic P, while inorganic P mainly existed in the occluded P (O-P) and Fe-P. Compared with the corresponding bulk soils, the rhizosphere soils contained lower total P and lower pH, the higher proportion of organic C, available P, proportion of organic P in the total P, proportion of active organic P in the organic P and proportion of Ca2-P in the total P. The contents of organic P, Ca2-P, Al-P and available P were significantly correlated with the contents of organic carbon. The results showed that the root uptake of P from deep soil, the return of dead leaves to the surface and migration of surface runoff were the main driving forces for the spatial differences of soil P in natural forest land.

  Impact of Long-term Fertilization Based on Meta-analysis on Stability of Soil Aggregates in Chinese Farmland TANG Yingying, TANG Xian, ZHAO Jianrong 2025, 41 (9):  117-126.  DOI: 10.11924/j.issn.1000-6850.casb2024-0614 Abstract + ( 65 )   HTML ( 2 )   PDF ( 1499KB )   ( 9 ) Soil aggregates are essential indicators of soil structure and fertility, significantly influenced by management practices, including the type of fertilizer used. This study aimed to investigate the effects of long-term fertilization on soil aggregate stability, measured by mean weight diameter (MWD), and to analyze the roles of various fertilization types, cropping systems, and soil types. We collected data from 48 published studies to create a comprehensive database on different fertilization practices and their impact on aggregate stability, including 292 independent paired data sets. Meta-analysis was employed to evaluate the effects of fertilization on soil aggregate stability. The findings revealed that, compared to no fertilization, the application of chemical fertilizers, organic fertilizers, and combined organic-inorganic fertilizers significantly enhanced soil aggregate MWD. Specifically, the impacts of combined organic-inorganic fertilization (an increase of 21.46%) and single organic fertilization (an increase of 17.17%) were 7.9 times and 6.4 times greater than that of single chemical fertilization (an increase of 6.22%), respectively. Additionally, the effect of straw application on MWD (27.79%) was significantly higher than that of manure (14.01%). Furthermore, the duration of fertilization was significantly negatively correlated with MWD, while annual precipitation, evaporation, and temperature were all significantly positively correlated with MWD. The optimal fertilization amounts were determined to be > 0.3 t/hm2 chemical fertilizers, 5-10 t/hm2 organic fertilizers, and 1-10 t/hm2 combination of organic and inorganic fertilizers, all of which significantly enhanced the stability of soil aggregates. Additionally, soil properties and environmental factors, such as pH, total nitrogen, total phosphorus, and total potassium, also influenced these effects. This study highlighted that the type of fertilization played a significant role in determining the stability of soil aggregates, underscoring the importance of organic fertilizers and combined organic-inorganic fertilization for improving soil structure. The results provided vital evidence for optimizing soil management practices.

  Meta-analysis of Total Organic Carbon in Agricultural Soils Under Biochar Amendment Model SONG Kai, GAO Yu, ZUO Yutian, LIU Yang 2025, 41 (9):  125-131.  DOI: 10.11924/j.issn.1000-6850.casb2024-0644 Abstract + ( 57 )   HTML ( 2 )   PDF ( 1429KB )   ( 10 ) Biochar, a carbon-rich material renowned for its distinctive physical properties, holds significant promise for improving soil texture. However, the performance of biochar-amended soils is contingent upon a variety of factors, including the inherent characteristics of the biochar, the prevailing soil conditions, and the specifics of the experimental framework employed. In this study, we undertook a comprehensive Meta-analysis to assess the impact of biochar application on soil organic carbon (SOC) content. By synthesizing literature published between 2013 and 2024, we meticulously screened 207 datasets derived from 84 studies. The results indicated that the enhancement of total organic carbon (TOC) content in soils after biochar application was more pronounced under specific conditions: when the biochar pH < 9, the soil type was sandy, and the initial organic carbon content was low. Notably, the maximum increase in soil TOC, an impressive 53.43%, was observed in sandy soils. Furthermore, with the increase of biochar application, a corresponding rise in soil TOC content was also documented. This study elucidated the influence of biochar on soil organic carbon, integrating factors such as biochar characteristics, soil conditions, and experimental parameters. Through this comprehensive analysis, we aimed to underscore the significant potential of biochar as a transformative agent for soil enhancement.

  Molecular Mechanism of ICE-CBF-COR-mediated Cold Tolerance Regulation in Plants Under Cold Stress: A Review ZHAO Guilong, TANG Jiaqi, HUANG Shuoqi, LI Rongtian, BU Qingyun, LU Zhenqiang 2025, 41 (9):  132-139.  DOI: 10.11924/j.issn.1000-6850.casb2024-0427 Abstract + ( 67 )   HTML ( 2 )   PDF ( 1316KB )   ( 9 ) Cold stress directly affects plant growth and development, and under extreme conditions, it may result in infertility or even lead to plant mortality. To investigate the molecular mechanisms by which plants respond to cold stress, this review synthesizes the diverse effects of cold stress on plants. It discusses the impact of cold stress on plant plasma membranes, the ICE-CBF-COR signaling pathway, plant hormones, and cellular metabolism. Additionally, recent advancements in understanding the mechanisms underlying plant cold tolerance are examined and discussed. This review aims to provide a foundation for the practical application of these findings in the genetic improvement of crops. Based on the above, the paper suggests that a multidisciplinary approach, incorporating genetic engineering, genetics, biochemistry, molecular biology, and bioinformatics should be employed to further explore the molecular mechanisms behind plant cold tolerance. Furthermore, potential future research directions in this field are proposed.

  Effects of Different Carbon Sources on Mycelium Growth and Extracellular Enzyme Activities of Agaricus bisporus A15 and W192 WANG Qian, XIAO Tingting, ZHANG Yuchen, ZHANG Jinjing, JUAN Jiaxiang, CHEN Hui, CHEN Mingjie, HUANG Jianchun 2025, 41 (9):  140-148.  DOI: 10.11924/j.issn.1000-6850.casb2024-0338 Abstract + ( 59 )   HTML ( 4 )   PDF ( 2021KB )   ( 6 ) The effects of different carbon sources on the mycelium growth and extracellular enzyme activities of A15 and W192 were investigated to understand the physiological requirements of carbohydrates of the two commercial Agaricus bisporus strains. Using different monosaccharides, disaccharides, oligosaccharides and polysaccharides as test materials, the mycelial growth rate, mycelial biomass and extracellular lignocellulase activities were determined. Glucose and fructose increased the mycelial biomass of A15 by 7.7%-30.8% and 23.1%-38.5%, respectively, while that of W192 was increased by 21.1%-36.8% and 26.3%-57.9%, respectively. Trehalose, sucrose, starch and cellulose increased the growth rate of mycelia; trehalose, maltose, sucrose, xylo-oligosaccharide, starch and cellulose increased mycelial biomass, among which 0.5% cellulose increased the mycelial biomass of A15 and W192 by 3.3 and 2.3 times, respectively. 10 carbon sources increased laccase, C1 and Cx activities of A15 and W192, respectively. All the 12 carbon sources inhibited β-GC activity of A15. However, in addition to xylan and lignin, the β-GC activity of W192 was increased. Trehalose, maltose, sucrose, xylo-oligosaccharide, starch and microcrystalline cellulose promote mycelial growth significantly. W192 may have higher carbon source demand or tolerance than A15.

  Invasion Trajectory and Control Prospects of Spartina alterniflora in Yellow River Delta YANG Ruimeng, YAN Guoyong 2025, 41 (9):  149-156.  DOI: 10.11924/j.issn.1000-6850.casb2024-0615 Abstract + ( 49 )   HTML ( 2 )   PDF ( 1703KB )   ( 7 ) Spartina alterniflora, as an alien invasive plant, has spread rapidly in the Yellow River Delta region, threatened native species, and damaged ecosystem health. This paper analyzes the invasion footprint of Spartina alterniflora in the Yellow River Delta by using remote sensing satellite image analysis techniques. It was found that after Spartina alterniflora completed colonization (before 2004), it quickly occupied the invaded habitat and rapidly spread (2004—2014). Although human intervention had suppressed the invasion speed of Spartina alterniflora and reduced the invasion area in the past decade (2014—2024), it still had the potential to make a comeback and reinvade. Therefore, we further classify and summarize the hypotheses of different stages of Spartina alterniflora invasion, compare various control measures, the prospect of scientific exploration of microbiological control of Spartina alterniflora in the future is proposed. The aim of this study is to provide a new way to prevent and control Spartina alterniflora invasion in the future.

  Study on Exogenous Ascorbic Acid Alleviating Toxicity of Atrazine to Beet Seedlings and Its Degradation Mechanism TONG Menglu, DING Yuling, JIANG Bingshan, WANG Zichen, PANG Caiwei, ZHOU Qin 2025, 41 (9):  157-164.  DOI: 10.11924/j.issn.1000-6850.casb2024-0311 Abstract + ( 51 )   HTML ( 2 )   PDF ( 1452KB )   ( 5 ) In order to explore the alleviating effect of exogenous ascorbic acid on the toxicity of beet seedlings under atrazine stress and the degradation mechanism of atrazine, using beet 'KWS1197' seeds as experimental materials, pot experiments combined with instrumental analysis were conducted to determine the effects of different concentrations of ascorbic acid on physiological and biochemical indexes of beet under atrazine stress, as well as the pesticide residues and degradation mechanisms in the soil. The results showed that atrazine stress inhibited the growth of beet seedlings, after spraying ascorbic acid, the toxicity of beet seedlings under atrazine stress was alleviated. Spraying 1mmol/L ascorbic acid had the most significant effect. The dry weight of the above-ground part and the underground part was 2.54 times and 3.43 times that of atrazine treatment group (AT treatment group). Chlorophyll content was increased by 42.5%. MDA content and electrolyte permeability were 53.57% and 65.01% of those in AT treatment group. SOD, POD and CAT enzyme activities were reduced by 60.5%, 61.3% and 51.1%, compared with AT treatment group. Spraying ascorbic acid can reduce the residual amount of atrazine in soil, and the higher the concentration, the more obvious the degradation effect. Four degradation products were found, and four possible degradation pathways of atrazine were further speculated. Ascorbic acid can improve the adverse effects of atrazine on the growth of beet seedlings, and the effect is significant at 1 mmol/L. This study provides a reliable reference for the degradation of atrazine.