Abstract: To improve the space and ground utilization of sunlight greenhouse, the authors studied the feasibility of Grifola frondosa cultivation under grape trellis in sunlight greenhouse. In early March, grow G. frondosa every 10 days under grape trellis, environmental factors and physical and chemical properties of G. frondosa of the four sowing periods were determined by temperature and humidity recorder, dry weighing method, Kjeldahl method and so on. From March to June, the daily average temperature range of grape leaf canopy and the humidity range was 15- 28℃ and 32%- 84% , respectively. The average temperature and humidity range at 20 cm above the ground was 13-28℃ and 55%-95%, respectively. The average temperature range at 10 cm under the ground was 15-26℃. The data showed that the requirements of temperature and humidity during the mushroom cultivation could reach those of grape in spring and summer. After the casing of the mushroom, water content of the media was 50%- 57% , and the pH value was acidic. The EC value increased since the formation of the primordium, while the carbon-nitrogen ratio continuously decreased with the final level of (36-38):1 at the end of the fruiting period. The cellulose and xylanase manifested the highest level during the primordium, while laccase activity reached its maximum during the fruiting stage. Amylase activity reached the highest level in the mycelial growth stage, followed by continuous decrease during fruiting and increase after maturation. The biological conversion rate of G. frondosa cultivation from early March using the present model could reach 40%, which was close to that of G. frondosa cultivation using standard imitative wild culture techniques.