Abstract: The great leaf wolfberry from Guangxi was used to study its biological characters. To take one-year-old and two-year-old branch of great leaf wolfberry from Guangxi as material for cutting propagation, we had observed the condition of take root of cuttings; and compared the condition of take root of one years old cuttings under different cover material and different port of cuttings and different hormone. Meanwhile we primarily studied cultivation techniques in the sunlight hothouse. The results showed: the two-year-old branch struck root faster and the number of root was more than one-year-old branch. The cuttings struck more roots when it was covered by plastic film than covered by sunshading net and constrast; the cuttings of middle port struck root fast and the number of root was the most among primary port and middle port and top port of abranch;the number of root of the cuttings that were treated with NAA300mg/L were the most among NAA100mg/L and NAA200mg/L and NAA300mg/L and IBA100mg/L and IBA200mg/L and IBA300mg/L.