Abstract: It is very important for the farmers and breeders to build a fast and dependable method of cultivars identification. By using SSR,20 apple cultivars were amplified by using 11 pairs of primers for genomic polymorphic analysis. SSR fragments of all pairs of primers were detected by polyacrylamide gel:163 loci were amplified and polymorphic percentage of amplified loci was 33.33%~63.16%. Based on the SSR results, calculation of the similarity coeffecient and also UPGMA cluster analysis showed that the apple cultivars could be classified into the four groups being similar to the traditional classification. And genotype map at a group of SSR loci of per cultivar was obtained. So genotype patterns of providing cultivars were firstly established. It would lay out a foundation for further genetype database, also indicating that SSR markers were suitable for early variety identification and plant breeder’s rights protection.