Abstract: In order to discuss the relationship between Fe and Mn, prevent the occurrence of nutrient disease on apple tree. Fe and Mn concentration in flowers and leaves of apple tree in various main product area of Shandong province were investigated. The results showed that Fe concentration in flowers were higher than that in leaves. Fe concentration in leaves of nutrient recycling period was the highest, Spring tip sopping period was the second, and the Autumn growing period was the lowest. Fe concentration in apple leaves with iron chlorosis changed little compared with no iron chlorosis. However, Mn concentration in flowers of apple trees were lower than that in leaves, and it appeared increasing from flower blooming period to nutrient recycling period. Mn concentration in apple leaves with iron deficiency were lower than that without iron chlorosis, the degree of reduction were the same as iron chlorosis in apple tree, and Fe/Mn ratio increased. Compared with normal apple orchard, Mn concentration increased and Fe/Mn ration decreased obviously, and Fe concentration changed little in corresponding to the degree of Internal Bark Necrosis. It suggested that Fe and Mn balance in apple tree play an important role for correction of Fe and Mn disorder.