Effect of Betaine on the Growth and Proliferation of Fibroblast Cell from Three-Yellow Broilers Embryos

Abstract

Abstract: 【Objective】This aim of this experiment was to study the effects of different concentration betaine on the micronucleus(MN) and the growth and proliferation of Three-Yellow chicken embryo fibroblast (CEF) in order to reveal the effects of mechanism of betaine on Three-Yellow broilers at cell level. 【Method】The Three-Yellow chicken embryo fibroblast cell (CEF) was isolated and cultured with supplement 10mM or 20mM betaine into culture medium. 【Results】Firstly, there were no significant effects on MN of Three-Yellow CEF by supplement 10mM or 20mM betaine into culture medium. It significantly increased the micronucleus rate of CEF to added 30mM, 40mM, 50mM betaine into culture medium, secondly, Betaine could effectively promote the growth and proliferation of Three-Yellow CEF when added 10mM or 20mM betaine into basic medium (DMEM+15%NBS). The rerult also showed that had the best proliferation of CEF in all groups when added 20mM betaine into basic medium. But betaine could restraint the growth and proliferation of Three-Yellow CEF when added 50mM betaine into basic medium. 【Conclusion】The result showed that low dose of Betaine could promote the growth and proliferation of Three-Yellow broilers embryo fibroblast, high dose of Betaine could hurt the division of Three-Yellow broilers embryo fibroblast.

CLC Number:

S853.74

Xiao Mei, Zhang tubao, Wu Lingfeng, An Lilong, Li Yuejian, Xu Yingmei, Wang Xibo. Effect of Betaine on the Growth and Proliferation of Fibroblast Cell from Three-Yellow Broilers Embryos[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 27-27.

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