Abstract: 【OBJECTIVE】Through regultating temperature difference of altitude, Gastrodiae can be cultivated in a feasible and economical way. 【METHODS】According to the Temperature Theory of the cultivation of Gastrodiae, researches on regulating the temperature difference of altitude were conducted, by observing and calculating the corresponding ground temperature of air temperature at different altitude levels. 【RESULTS】The most effective way of Gastrodiae cultivation in Yichang is to set up breeding plantation at the altitude of 1000m, hybriding area at 100m, seed multiplication farm at 500m and GAP base for commercial gastrodiea at above 800m. In this way, the cultivating period of sexual seed tubers was shortened by 12 months and 12 months was saved for the cultivation for commercial tubers both with good quality and high yield. Besides, two novel hybrid species- Er No 1 and No 2 Gastrodiae were obtained by conforming the florescence of G. elata Bl. f. elata and G. elata Bl. f. glauca S. Chow.【CONCLUSION】 Regulating the temperature difference of altitude is an efficacious and economical way of cultivating Rhizoma Gastrodiae.