Abstract: Physiological change in cooling storage at 0℃ of six varieties of seeding of strawberry including big and small size were studied. The result indicated that the dry substance of seeding of strawberry decreased totally, the soluble sugar, the starch content and activity of CAT increased primarily then decreased, the turning point was sixty days after cooling storage, the nitrogen of saltpeter form decreased primarily then increased and decreased at last, the activity of POD increased all the time, the best variety of seeding of strawberry for cooling storage was big seeding of Hokowase,the big seeding was better than the small seeding for cooling storage.