To enhance the ability of meteorological service for agriculture and ensure the safety of food production, based on the fixed-point observation data of light heat water resources, crop growth and yield from 1981 to 2017 in various counties (districts) of Wuwei City, the change and matching characteristics of light heat water resources and their impacts on crops were analyzed by using climatic tendency rate, correlation coefficient method and SPSS13.0 statistical software. The results showed that: (1) 1981-2017, the ≥10℃ accumulated temperature and sunshine hours increased significantly, and the increase range and stability decreased gradually from north to south, during the period, the precipitation slightly increased, the light, heat and water resources increased steadily in the middle and north, the suitable climate region for crop growth were enlarged; (2) the hydrothermal coefficient showed a decreasing trend, the dryness increased slightly except in Minqin, the light temperature product showed an extremely significant increase trend, while the light temperature ratio showed an extremely significant increase in Minqin and Liangzhou, but an extremely significant decrease in Gulang and Tianzhu, the light temperature matching was beneficial to the formation and development of crop yield; (3) according to the correlation coefficient, the increase of light and heat resources promoted the growth and fruiting process of cool-loving crops, and the matching of light and heat could increase the grain weight and economic yield of warm-loving crops. According to the matching of light, heat and water resources in the whole city, Tianzhu and Gulang shallow mountainous are suitable for the growth of cool crops, and their climatic conditions are also suitable for developing animal husbandry and forestry. Minqin, Liangzhou and Gulang flat land are suitable for growing warm-loving crops, such as maize, sweet sorghum, millet, sunflower and melon fruits, and for developing characteristic agriculture.