Ipomoea aquatica: Purification of Aquaculture Water Contaminated by Methomyl and Effect on Fish Growth

doi:10.11924/j.issn.1000-6850.casb20190600303

Abstract

Abstract:

Methomyl is a highly toxic pesticide, which can contaminate aquaculture water by means of surface leaching. To study the purification efficiency of water spinach on methomyl in the water body and other water quality indexes and investigate the impact of water spinach on tilapia, we took methomyl, tilapia, water spinach and indoor cultivation system as test materials to measure the water quality indexes according to the Water and Wastewater Monitoring Analysis Methods. The methomyl concentration in the water body was measured by the Liquid Chromatograph Mass Spectrometer (LC-MS), and the glutathione (GSH) content (the DTNB method), superoxide dismutase (SOD) content (WST-1 method) and catalase (CAT) content (the Ultraviolet Spectroscopy) were measured using the assay kit. The results showed that the growth rate of tilapia decreased with the increase of methomyl concentration in water. Methomyl contamination led to increasing the oxidative pressure of the tilapia liver, and caused the changes of SOD, CAT and GSH. Water spinach could effectively reduce the content of methomyl in the aquaculture water through body absorption, it purified TN, NH₄ ⁺-N, NO₃ ^--N and NO₂ ^--N in the water contaminated by methomyl to a certain extent and reduced the oxidative stress of the tilapia liver. The study reveals that water spinach grown in water can effectively reduce the pollution of methomyl and ensure fish growth.

Key words: water spinach, methomyl, aquatic water, purification, genetically improved farmed tilapia

CLC Number:

S964.3
X52

Wang Yuqin, James P. Mulbah, Meng Shunlong, Zheng Yao, Chen Jiazhang, Wu Wei. Ipomoea aquatica: Purification of Aquaculture Water Contaminated by Methomyl and Effect on Fish Growth[J]. Chinese Agricultural Science Bulletin, 2020, 36(3): 147-153.

Figures/Tables 5

References 25

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	A组(Con)	B组(2 μg/L)	C组(20 μg/L)	D组(200 μg/L)
试验前平均体重/g	5.32±0.06	5.31±0.07	5.09±0.09	5.25±0.1
试验后平均体重/g	33.52±0.35	31.79±1.37	30.11±1.24	28.83±1.08
试验后总体重/g	529.39±9.25	498.67±29.77	491.24±22.37	449.48±28.38
FCR/%	1.06±0.01^b	1.13±0.06^ab	1.2±0.05^ab	1.27±0.06^a
SGR/(%/d)	3.06±0.02^a	2.97±0.08^ab	2.95±0.06^ab	2.83±0.08^b

	A组(Con)	B组(2 μg/L)	C组(20 μg/L)	D组(200 μg/L)
试验前平均体重/g	5.32±0.06	5.31±0.07	5.09±0.09	5.25±0.1
试验后平均体重/g	33.52±0.35	31.79±1.37	30.11±1.24	28.83±1.08
试验后总体重/g	529.39±9.25	498.67±29.77	491.24±22.37	449.48±28.38
FCR/%	1.06±0.01^b	1.13±0.06^ab	1.2±0.05^ab	1.27±0.06^a
SGR/(%/d)	3.06±0.02^a	2.97±0.08^ab	2.95±0.06^ab	2.83±0.08^b