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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 57-61.doi: 10.11924/j.issn.1000-6850.2013-3119

• 林学 园艺 园林 • 上一篇    下一篇

红花檵木叶片再生不定芽的研究

符红艳 于晓英   

  • 收稿日期:2013-11-27 修回日期:2014-01-20 出版日期:2014-06-05 发布日期:2014-06-05
  • 基金资助:
    国家自然基金面上项目“DREB和XET基因聚合表达的分子基础”(31071826)；湖南省高等学校科学研究项目重点项目“红花檵木叶色高温返青的分子机理研究”(10A057)；湖南省教育厅省高等学校产业化培育项目“红花檵木新品种繁育及生产基地建设”(07CY001)。

Adventitious Bud Regeneration from Leaves of Loropetalum chinense var. rubrum Cultivars

  • Received:2013-11-27 Revised:2014-01-20 Online:2014-06-05 Published:2014-06-05

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摘要： 为了建立红花檵木叶片高效的离体再生体系，解决其基因工程育种的问题，以红花檵木叶片为外植体，探讨了品种、光照条件、激素等因素对叶片离体再生的影响。结果表明：不同品种叶片再生不定芽能力不同，‘玫红细叶青’再生能力最强，其次是‘长叶玫红’，‘花叶2号’叶片再生最难。白光和红光有利于叶片再生，70天后都有不定芽出现。其中白光下的诱导率达50%，红光下诱导率达25%。叶片近轴面接触培养基，再生效率高。适合诱导‘玫红细叶青’再生不定芽适宜培养基为MS+6-BA 1.0 mg/L+ NAA 0.5 mg/L其不定芽分化率为41.6%，适合‘长叶玫红’不定芽分化的组合是MS+6-BA 1.0 mg/L+ NAA 1.0 mg/L其不定芽分化率为33.3%。

关键词: 旋心异跗莹叶甲, 旋心异跗莹叶甲, 毒力测试, 防治效果, 化学农药, 生物农药

Abstract: The aims were to establish Loropetalum chinense var. rubrum cultivars efficient regeneration system and lay a foundation for solving the problem of gene engineering breeding. In this paper, the regeneration system of Loropetalum chinense var. rubrum cultivars in vitro was studied with leaves as explants. Some factors such as different cultivars, illumination condition, and hormone concentration and so on were investigated. The results showed the regeneration efficiency of leaves was different. The highest one was in L. chinense var. rubrum‘MeihongXiyeqing’, followed L. chinense var. rubrum‘Changye Meihong’and variegated variation of L. chinense var. rubrum No.2. It could have higher regeneration efficiency when the leaves with the treatment by white light and red light and laid on the medium in adaxial side down, after 70 days, their regeneration frequency were 50% and 25%. The optimal bud regeneration medium from leaves of L. chinense var. rubrum‘Meihong Xiyeqing’was MS + 6- BA 1.0 mg/L + NAA 0.5 mg/L, the induction frequency was 41.6% . The appropriate medium for adventitious bud induction from leaves of L. chinense var. rubrum‘Changye Meihong’was MS+6-BA1.0 mg/L+NAA1.0 mg/L, the induction frequency was 33.3%.