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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 52-54.

所属专题： 生物技术； 马铃薯

• 生物技术科学 • 上一篇    下一篇

优良木薯品种“ND-50”的组织培养

何婷1,2,孙月芳1,2,王亦菲1,2,陆瑞菊1,2,邹磊1,2,何婷1,2,孙月芳1,2,王亦菲1,2,陆瑞菊1,2,邹磊1,2,   

  • 收稿日期:2009-09-14 修回日期:2009-10-23 出版日期:2010-02-05 发布日期:2010-02-05

Tissue Culture of Manihot esculenta Crantz ND-50

  • Received:2009-09-14 Revised:2009-10-23 Online:2010-02-05 Published:2010-02-05

PDF (PC)

808

摘要：

以木薯品种“ND-50”带腋芽的茎段为外植体进行组织培养，在芽的增殖和继代生长两个阶段进行观察研究。结果表明，MS+6-BA1.0mg/L+IAA1.0mg/L培养基较适合作为组培苗的增殖培养基；MS+KT1.0mg/L+NAA0.05mg/L作为组培苗的壮苗培养基较好。通过“ND-50”组织培养研究，为木薯的快速繁育和推广提供参考依据。

关键词: 大豆, 大豆, 连作, 根际, 土壤养分

Abstract:

The research aimed to study the tissue culture of varieties ND-50 of cassava by using its node sections taken as explants including multiplication and subculture .The results showed that MS+6-BA1.0mg/L+IAA0.1mg/L was suitable for the multiplication medium of tissue culture seedling. MS+KT1.0mg/L+NAA0.05mg/L was better for subculture medium. The tissue culture of cassava ND-50 could provide references for its seedlings rapid propagation and promotion.