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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 56-60.doi: 10.11924/j.issn.1000-6850.2012-0585

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

胎生蜥蜴消化道4种内分泌细胞的免疫组织化学研究

吴昊 李淑兰 刘志涛 刘鹏 赵文阁   

  • 收稿日期:2012-02-27 修回日期:2012-04-13 出版日期:2012-08-15 发布日期:2012-08-15
  • 基金资助:

    哈尔滨师范大学科技发展预研项目 “极北鲵发育形态学和免疫组织化学研究（09XYG-08）”；国家自然科学基金资助项目“胎生蜥蜴生活史特征的地理变异和对寒冷气候适应性的研究（31172079）”

Immunohistochemical Study of the Four Kinds of Endocrine Cells in the Digestive Tract of Lacerta vivipara

  • Received:2012-02-27 Revised:2012-04-13 Online:2012-08-15 Published:2012-08-15

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摘要：

为了探索胎生蜥蜴消化道内分泌细胞的形态与分布规律。应用胃泌素(Gas)、胰高血糖素(Glu)、胰多肽(PP)和P-物质(SP) 4种特异性抗血清，对胎生蜥蜴(Lacerta vivipara)消化道内分泌细胞进行了免疫组织化学定位研究和形态学观察。用免疫组织化学ABC法(avidin-biotin compex method)以揭示其消化道内分泌细胞的分布规律及特点。结果表明：Gas细胞分布在贲门、幽门和小肠，其中以十二指肠处分布密度最高，贲门次之。Glu细胞主要分布在幽门和小肠前段，小肠后段偶见，并且幽门处的分布密度明显高于其他部位。在幽门、十二指肠和回肠都检测到了PP细胞，并且在十二指肠分布最多。仅在幽门部检测到了少量的SP细胞。4种内分泌细胞以圆形和锥体形为主，它们广泛分布于上皮细胞之间、腺泡上皮细胞之间及上皮细胞基部。上述内分泌细胞的分布特点可能与其食性、食物组成和生活环境有关。

关键词: 作用, 作用

Abstract:

To clarify the morphological features and region distribution of the endocrine cells in the digestive tract of the Lacerta vivipara. The localization and morphology of endocrine cells in the digestive tract of Lacerta vivipara were studied with gastrin (Gas), Glucagon (Glu), pancreatic polypeptide (PP) and substance P-(SP) four kinds of specific antisera. Immunohistochemical ABC method (avidin-biotin compex method) was used to reveal the law and characters of endocrine cells in the digestive tract. The results showed that Gas cells located in the cardia, pylorus and small intestine, with the highest density in the duodenum, followed by the cardia. Glu cells were mainly in the pylorus and the anterior small intestine, the back of the small intestine occasionally, and the pylorus’s distribution of density was significantly higher than any other parts. PP cells were detected in the pylorus, duodenum and ileum, with the largest distribution in the duodenum. SP cells were only found in the pylorus. The four kinds of endocrine cells were mainly in the shape of round and cone-shaped, which widely lay between epithelial cells, between glandular epithelial cells and at the bottom of epithelia. The characters of the distribution of the endocrine cells might be related to the feeding habits, the food composition and the living environment.