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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (15): 105-111.doi: 10.11924/j.issn.1000-6850.2013-2482

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

秤锤树愈伤组织诱导和生长的初步研究

夏清 吴雅静 邵晨 田兴军   

  • 收稿日期:2013-09-18 修回日期:2013-10-18 出版日期:2014-05-25 发布日期:2014-05-25
  • 基金资助:
    江苏省林业三新工程项目 “工厂化苗木繁殖平台的构建及创新试验” (LYSX[2012]02)。

Primary Studies on the Induction and Growth of Callus of Sinojackia xylocarpa Hu

  • Received:2013-09-18 Revised:2013-10-18 Online:2014-05-25 Published:2014-05-25

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摘要： 为了研究秤锤树外植体的最佳采集时间和愈伤组织的诱导及生长状况，以秤锤树的叶片为外植体，选择WPM+2,4-D 2.0 mg/L+6-BA 1.0 mg/L+蔗糖20 g/L+琼脂8 g/L作为培养基，研究不同月份取材和不同灭菌时间的处理对秤锤树愈伤组织诱导的影响，以及比较不同灭菌时间和盐胁迫条件下秤锤树愈伤组织的生理生化指标和生长情况。结果表明：秤锤树愈伤组织诱导的最佳取材月份是4月，该时期外植体的污染率最低；最佳灭菌方式为无菌水3次+75%酒精30 s+0.1%升汞1~5 min+无菌水3次。不同灭菌时间处理对愈伤组织生化指标影响明显，其中用升汞进行1 min、3 min表面消毒处理诱导产生的愈伤组织中可溶性糖显著高于5 min表面消毒处理诱导的愈伤组织中可溶性糖的含量，用升汞进行1 min、3 min表面消毒处理诱导产生的愈伤组织中可溶性蛋白显著低于5 min表面消毒处理诱导的愈伤组织中可溶性蛋白的含量，不同灭菌时间对SOD和POD的活性影响不显著；而用升汞进行3 min和5 min表面消毒诱导产生的愈伤组织表现出更好的耐盐性。

关键词: 评估, 评估

Abstract: In order to primary studies on the collection time of explants and induction and growth of callus of tender leaf (Sinojackia xylocarpa Hu), the leaves used as explants with WPM+2,4-D 2.0 mg/L+6-BA 1.0 mg/L+ sucrose 20 g/L+ agar 8 g/L as medium for the experiment to study collection and disinfected time on tender tissue culture. The results showed that the optimal time of explants collection for tender tissue culture was April. During this period, the contamination rate of explants was the lowest. The optimal sterilizing treatment for the tender leaves was 75% Alcohol 30 s+0.1% HgC12 1-5 min. Different disinfected time had a distinctly effect on physiology and biochemistry of callus. The soluble sugar in callus inducted with 1 min and 3 min disinfected time were significantly higher (P<0.05) compared with 5 min disinfected time. The soluble protein in callus inducted with 1 min and 3 min disinfected time was significantly lower (P<0.05) than that in callus with 5 min disinfected time. Different sterilization time had no significantly effect on the activity of peroxidase and superoxide dismutase. The callus inducted with 3 min and 5 min disinfected time had a stronger tolerance (P<0.05) of salt stress compared with the callus inducted with 1 min disinfected time.