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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (7): 66-71.doi: 10.11924/j.issn.1000-6850.2014-2064

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

西瓜抗枯萎病育种的分子辅助选择

王吉明,尚建立,董亚玲,薛盈盈,马双武   

  1. (中国农业科学院郑州果树研究所,郑州 450009）
  • 收稿日期:2014-07-29 修回日期:2015-02-28 接受日期:2014-12-03 出版日期:2015-04-03 发布日期:2015-04-03
  • 通讯作者: 王吉明
  • 基金资助:
    “十二五”农村领域国家科技计划课题“西瓜种质资源创新利用”(2013BAD01B04-21)。

Study on Molecular Marker Assisted Selection for Fusarium Wilt Resistance Breeding in Watermelon

Wang Jiming, Shang Jianli, Dong Yaling, Xue Yingying, Ma Shuangwu   

  1. (Zhengzhou Fruit Research Institute, CAAS, Zhengzhou 450009)
  • Received:2014-07-29 Revised:2015-02-28 Accepted:2014-12-03 Online:2015-04-03 Published:2015-04-03

摘要： 利用中国国内公开发表的西瓜抗枯萎病生理小种1基因紧密连锁标记4451_fon，首次对抗枯萎病育种创新的二倍体和四倍体种质进行分子鉴定。结果表明，全部二倍体单系和除编号D16、D17外的四倍体单系均表现为抗病基因型，判定为抗病材料，与人工接种鉴定结果完全一致；分子鉴定还表明，这些抗病单系为抗病纯合体，在以后的自交纯化过程中抗性基因不再分离和丢失，因此不必继续对其自交后代进行抗性选择。研究结果认为dCAPS标记4451_fon可以作为西瓜抗枯萎病分子辅助选择(molecular marker assisted selection，MAS)的重要工具，能够实现提高选择效率、加速育种进程的目的。

关键词: 水杨酸, 水杨酸, 茉莉酸, 乙烯, 抗病, 相互作用

Abstract: In this report, study on molecular identification of Fusarium wilt resistance for diploid and tetraploid watermelon breeding lines using dCAPS marker “4451_fon” which published in 2013 was carried out. The results showed that all diploid lines and tetraploid lines except No.D16 and D17 were Fusarium wilt resistant genetype material, could be identified as having Fusarium wilt resistance, and the result was in accordance with artificial inoculation identification. Molecular identification results also showed that those resistant lines were homozygote, so Fusarium wilt resistant gene would not separate when lines self-crossed, and there was no necessary carry out single resistant plant selection in their progeny population. The final conclusion were that dCAPS Marker “4451_fon” could be applied to molecular marker assisted selection for Fusarium wilt resistance breeding in watermelon to increase breeding selection efficiency and enhance breeding progress.