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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (13): 19-22.doi: 10.11924/j.issn.1000-6850.casb14110076

• 林学 园艺 园林 • 上一篇    下一篇

南方红豆杉紫杉醇含量的时空变异

房耀欣,花奕蕾,田兴军   

  1. 南京大学生命科学学院,210046,南京大学生命科学学院,210046,南京大学生命科学学院,210046
  • 收稿日期:2014-11-12 修回日期:2015-03-26 接受日期:2015-01-23 出版日期:2015-06-02 发布日期:2015-06-02
  • 通讯作者: 房耀欣
  • 基金资助:
    中国科学院战略性先导科技专项“应对气候变化的碳收支认证及相关问题”(XDA05050204)；国家自然科学基金“土壤有机物分解酶(CBHI)的动态变化、功能特征及其调控”(40971151)；教育部博士点基金“亚热带森林生态系统土壤漆酶的功能特征、动态变化及其调控”(20110091110018)。

Spatial Variation of the Taxol Content in Taxus chinensis var. mairei

  • Received:2014-11-12 Revised:2015-03-26 Accepted:2015-01-23 Online:2015-06-02 Published:2015-06-02

摘要： 红豆杉属植物含有的紫杉醇因具有抗癌特性逐渐成为各国研究的热点。由于植物体内紫杉醇含量很低,而市场对紫杉醇需求不断增大,供需矛盾尤为突出。本研究的目的是试图搞清植物体各部分紫杉醇的空间分布,旨在为合理开发红豆杉植物资源提供科学依据。本研究采用高效液相色谱法(HPLC),对同一株成熟的南方红豆杉不同时期的叶、皮、枝及背光处树叶中紫杉醇含量进行测定。结果表明,随着植物生长的时间变化,树皮中紫杉醇含量(11mg.kg-1-17mg.kg-1)逐渐增高且最多,枝叶中紫杉醇含量(枝13mg.kg-1-18mg.kg-1,叶4mg.kg-1-14mg.kg-1)在下降,但新生的枝叶(枝18 mg.kg-1,叶14 mg.kg-1)却比新皮中(11 mg.kg-1)紫杉醇含量高；同时光照强的叶中紫杉醇含量(26 mg.kg-1)比背光处叶含量(2 mg.kg-1)明显高。对于产业化提取红豆杉中紫杉醇,笔者建议可以利用可再生、生物量大的新生枝叶取代树皮获得更多的紫杉醇产品。

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Abstract: The study on spatial distribution of taxol in plant could provide a scientific basis for reasonable development of Taxus chinensis plant resource. This study investigated the paclitaxel content in leaves, barks and branches of different periods and the taxol content in the leaves under light condition in a plant of Taxus chinensis var. mairei, with high performance liquid chromatography (HPLC). The results showed that the taxol content in the bark (11-17 mg/kg) gradually increased, taxol content in branches (13-18 mg/kg) and leaves (4-14 mg/kg) decreased with accumulated time. The taxol content in the bark of the new branches (18 mg/kg) and in new needles (14 mg/kg) was higher than that in old ones. And taxol content in the leaves under light condition (26 mg/kg) was significantly higher than that in dark condition (2 mg/kg). For industrial extraction of taxol, it suggests that new branches and leaves which are renewable and have high biomass could be used to get more products of taxol.