上海梅山猪封闭群内源性逆转录病毒的检测及亚型分析

doi:10.11924/j.issn.1000-6850.casb15010020

中国农学通报 ›› 2015, Vol. 31 ›› Issue (14): 39-43.doi: 10.11924/j.issn.1000-6850.casb15010020

所属专题：生物技术；畜牧兽医

上海梅山猪封闭群内源性逆转录病毒的检测及亚型分析

吴鋆龙^1,2,3,张德福^2,3,吴彩凤^2,3,张树山^2,3,王昕^1,2,3,戴建军^2,3

(¹上海海洋大学水产与生命学院,上海 201306；²上海市农业科学院畜牧兽医研究所,上海 201106；³上海农业遗传育种重点实验室动物遗传工程研究室,上海 201106)

收稿日期:2015-01-05 修回日期:2015-03-05 接受日期:2015-03-16 出版日期:2015-06-02 发布日期:2015-06-02
通讯作者: 戴建军
基金资助:
上海市科委科技兴农专项“克隆和冷冻保存技术在上海白猪保种、育种中的应用”(133919N1700)；国家转基因生物新品种培育科技重大专项“不饱和脂肪酸、植酸酶转基因“环保猪”新品系的培育”(2009ZX08006-014B)，“高繁殖力转基因猪新品种培育”(2014ZX08006-005)；上海市农委青年人才成长计划“基于TALENs技术的猪外源基因定点整合方法的建立”（沪农青字2014-1-32），“甲基化修饰状态对猪体细胞克隆效率的影响”（沪农青字2014-1-33）。

Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai

Wu Yunlong^1,2,3, Zhang Defu^2,3, Wu Caifeng^2,3, Zhang Shushan^2,3, Wang Xin^1,2,3, Dai Jianjun^2,3

(¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306;²Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106; ³Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-genetics and Breeding, Shanghai 201106)

Received:2015-01-05 Revised:2015-03-05 Accepted:2015-03-16 Online:2015-06-02 Published:2015-06-02

摘要/Abstract

摘要： 为检测分析上海梅山猪携带猪内源性逆转录病毒(porcine endogenous retrovirus, PERV)的情况，为该猪种作为器官移植供体的生物安全性提供现实依据，笔者从上海嘉定区梅山猪育种中心的梅山猪封闭群内随机采集45头猪的耳组织，并构建耳成纤维细胞系，利用PCR检测PERV前病毒核心蛋白基因(gag)、多聚酶基因(pol)以及囊膜基因(env)的基因组DNA，利用RT-PCR的方法检测mRNA表达状况。结果发现被检测的45份样品均携带完整的3种亚型的PERV前病毒DNA；RT-PCR结果显示，45份样品中有40份同时有PERV-A和PERV-B两种亚型的表达，还有5份样品仅检测到PERV-B亚型的表达，未检测到PERV-C亚型的表达。鉴于生物安全性方面的考虑，该猪种能否作为人异种移植的供体仍有待进一步商榷。

关键词: 组织特异性启动子, 组织特异性启动子, 作物基因工程, 研究进展

Abstract: In order to provide actual evidence of biological safety for using Meishan pigs for donor organ transplantation, existence and subtype of porcine endogenous retrovirus (PERV) in Meishan pigs were investigated and analyzed respectively. Tissues of 45 pig ears were collected randomly from Meishan pig breeding center in Jiading District, Shanghai. Ear fibroblast cell lines from these tissues were established. The existence of core protein gene (gag), polymerase gene (pol) and capsule membrane gene (env) from PERV provirus in genomic DNA was detected by PCR and expression of mRNA of each gene was tested by RT-PCR. The results showed that three subtypes PERV provirus existed in all 45 DNA samples. Results of RT-PCR showed that PERV-A and PERV-B both expressed in 40 samples, subtype PERV-B expressed only in 5 samples, and subtype PERV-C was not found in this study. In consideration of biological safety, whether these pigs can be used as human xenotransplantation donors still need further discussion.

吴鋆龙,张德福,吴彩凤,张树山,王昕,戴建军. 上海梅山猪封闭群内源性逆转录病毒的检测及亚型分析[J]. 中国农学通报, 2015, 31(14): 39-43.

Wu Yunlong,Zhang Defu,Wu Caifeng,Zhang Shushan,Wang Xin and Dai Jianjun. Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai[J]. Chinese Agricultural Science Bulletin, 2015, 31(14): 39-43.

参考文献

[1] Kuddus R H, Metes D M, Nalesnik M A, et al. Porcine cell microchimerism but lack of productive porcine endogenous retrovirus (PERV) infection in naive and humanized SCID-beige mice treated with porcine peripheral blood mononuclear cells[J]. Transplant immunology,2004,13(1):15-24.
[2] Denner J. Infectious risk in xenotransplantation-what post-transplant screening for the human recipient[J]. Xenotransplantation,2011,18(3):151-157.
[3] van der Laan L J, Lockey C, Griffeth B C, et al. Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice[J]. Nature,2000,407(6800):90-94.
[4] Wilson C A. Porcine endogenous retroviruses and xenotransplantation[J]. Cell Mol Life Sci,2008,65(21):3399-3412.
[5] Wu J, Ma Y, Lv M, et al. Large-scale survey of porcine endogenous retrovirus in Chinese miniature pigs[J]. Comp Immunol Microbiol Infect Dis,2008,31(4):367-371.
[6] 吴健敏,阳玉彪,郭艳茹,等.中国巴马小型猪封闭群内源性反转录病毒存在状况分析[J].中国预防兽医学报,2005,27(5):391-393.
[7] 唐赛涌,王勃,陆林根.上海地区梅山猪的历史发展与保种现状[J].中国猪业,2013(S1):69-71.
[8] Donna E, Akiyoshi M D, Zhu H, et al. Identification of a full-length cDNA for an endogenous retrovirus of miniature swine[J]. Journal of Virology,1998,72(2):4503-4507.
[9] Wilson C A, Wong S, Muller J, et al. Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells[J]. Journal of Virology,1998,72(4):3082-3087.
[10] Ibold Y, Lubke C, Pelz S, et al. Effect of different ascorbate supplementations on in vitro cartilage formation in porcine high-density pellet cultures[J]. Tissue Cell,2009,41(4):249-256.
[11] Le Tissier P, Stoye J P, Takeuchi Y, et al. Two sets of human-tropic pig retrovirus[J]. Nature,1997,389(6652):681-682.
[12] Takeuchi Y, Patience C, Magre S, et al. Host range and interference studies of three classes of pig endogenous retrovirus[J]. Journal of Virology,1998,72(12):9986-9991.
[13] Patience C, Takeuchi Y, Weiss R A. Infection of human cells by an endogenous retrovirus of pigs[J]. Nature Medicine,1997,3(3):282-286.
[14] Meije Y, Toenjes R R, Fishman J A. Retroviral restriction factors and infectious risk in xenotransplantation[J]. American Journal of Transplantation,2010,10(7):1511-1516.
[15] Kimsa M, Strzalka-Mrozik B, Kimsa M, et al. Porcine endogenous retroviruses in xenotransplantation-molecular aspects[J]. Viruses,2014,6(5):2062-2083.
[16] 王亚楠,刘静,刘榜,等.中国两头乌猪品种内源性逆转录病毒基因研究[J].中国实验动物学报,2013(3):70-74.
[17] 高勤学,杨晓志,周俊,等.姜曲海猪内源性逆转录病毒及亚型的检测与分析[J].江苏农业科学,2011(4):247-249.
[18] 邢晓为,薛立群,莫朝辉,等.湖南大围子猪内源性逆转录病毒的研究[J].中南大学学报:医学版,2006,31(6):838-842.
[19] Wilson C A, Wong S, VanBrocklin M, et al. Extended analysis of the in vitro tropism of porcine endogenous retrovirus[J]. Journal of Virology,2000,74(1):49-56.
[20] Karlas A, Irgang M, Votteler J, et al. Characterisation of a human cell-adapted porcine endogenous retrovirus PERV-A/C[J]. Annals of Transplantation,2010,15(2):45-54.
[21] Wood J C, Quinn G, Suling K M, et al. Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine[J]. Journal of Virology,2004,78(5):2494-2501.

上海梅山猪封闭群内源性逆转录病毒的检测及亚型分析

Investigation and Analysis of the Subtype of Porcine Endogenous Retrovirus in Breeding Base of Meishan Pigs in Shanghai

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