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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (22): 133-139.doi: 10.11924/j.issn.1000-6850.casb15120079

所属专题： 资源与环境； 园艺

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

野生古茶树资源主要生化成分多样性分析

杨兴荣,矣 兵,李友勇,段志芬,杨毅坚,尚卫琼,杨盛美,蒋会兵,李 慧,孙雪梅,刘本英   

  1. (云南省农业科学院茶叶研究所/云南省茶树种质资源创新与配套栽培技术工程研究中心,云南勐海 666201）
  • 收稿日期:2015-12-14 修回日期:2016-07-22 接受日期:2016-01-25 出版日期:2016-08-09 发布日期:2016-08-09
  • 通讯作者: 刘本英
  • 基金资助:
    国家自然科学基金项目“云南珍稀野生茶树资源的遗传多样性及特异资源发掘”(31160175)；国家自然科学基金项目“云南大叶茶资源核心种质构建及优异种质筛选”(31440034)；国家农作物种质资源平台项目“国家茶树种质资源平台（勐海）”(2013-065)；农业部物种资源保护（农作物）项目“大叶茶树种质资源收集鉴定编目繁殖更新与保存分发利用”(2014NWB028)；云南省农业科学院专项“茶树资源分圃改造及资源收集保存与种质创制”(YAAS2015ZY003)；云南省人才培引计划项目“云南省技术创新人才培养对象”(2011CI068)。

Diversity Analysis of Main Biochemical Components of Wild Ancient Tea Tree Resources

Yang Xingrong, Yi Bing, Li Youyong, Duan Zhifen, Yang Yijian, Shang Weiqiong, Yang Shengmei,Jiang Huibing, Li Hui, Sun Xuemei, Liu Benying   

  1. (Tea Research Institute, Yunnan Academy of Agricultural Sciences/ Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation, Menghai Yunnan 666201)
  • Received:2015-12-14 Revised:2016-07-22 Accepted:2016-01-25 Online:2016-08-09 Published:2016-08-09

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摘要： 野生古茶树遗传多样性最为丰富，是最具有保护和研究价值的初级茶树种质资源。以23份野生古茶树资源为供试材料，采摘夏梢1芽2叶制备蒸青茶样，进行水浸出物、茶多酚、氨基酸总量、咖啡碱和各儿茶素组分含量等生化成分多样性分析。结果显示：水浸出物变幅为43.65%~51.19%，高水浸出物(＞45.00%)资源有21份；茶多酚变幅为12.02%~38.60%，高茶多酚(＞25.00%)资源有16份；氨基酸变幅为1.46%~3.41%；咖啡碱变幅为2.17%~4.91%；儿茶素总量变幅为15.52%~25.89%；非酯型儿茶素变幅为5.09%~9.84%；酯型儿茶素变幅为5.02%~16.57%，其中EGCG变幅为2.87%~10.01%，高EGCG(＞8.00%)资源有4份；除茶多酚和氨基酸生化指标外，酚氨比也可以作为茶树品种适制性生化指标，初筛选出适制绿茶资源有5份，红绿茶兼制资源有11份，适制红茶资源有7份。供试23份野生古茶树资源内含物质丰富，可以作为进一步深入研究的重要资源，也为有针对性地合理开发利用和培育茶树品种提供优良资源材料。

关键词: 大麦, 大麦, 农艺性状, 中亲优势, 配合力

Abstract: Wild ancient tea trees have abundant genetic diversity, and they are the primary tea tree germplasm resource with high conservation and research value. In this study, 23 accessions of wild ancient tea tree resources were selected as test materials, and summer new shoots with one bud and two leaves were picked to make steamed samples, and then, the authors analyzed the diversity of biochemical components including water extract, tea polyphenols, total free amino acid, caffeine, various catechins composition and so on. The results showed that water extract was between the range of 43.65% to 51.19%, and there were 21 accessions of resources that contained high water extract (>45.00%). The tea polyphenols content was between the range of 12.02% to 38.60%, and there were 16 accessions with high tea polyphenols (>25.00%) materials. The variation of amino acids was between the range of 1.46% to 3.41%, the caffeine was between the range of 2.17% to 4.91%, the total catechins was between the range of 15.52% to 25.89%, the non-esters catechins was between the range of 5.09% to 9.84%, the esters-catechins was between the range of 5.02% to 16.57%, and the EGCG variation was between the range of 2.87% to 10.01%, with 4 high EGCG (>8.00%) accessions of resources. Except the tea polyphenols and the amino acid, the phenol ammonia could also be used as a biochemical index of tea varieties’ processing suitability. In the primary selection, 5 accessions of resources were optimum to make green tea, 11 were optimum to make black and green tea, and 7 were optimum to make black tea. The 23 accessions of the tested wild ancient tea tree resources contained abundant biochemical components, and could be used as important resources for a further in-depth study, as well as for a targeted development of tea variety cultivation.

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