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中国农学通报 ›› 2016, Vol. 32 ›› Issue (23): 1-5.doi: 10.11924/j.issn.1000-6850.casb16050005

所属专题: 生物技术 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

仔猪感染PRRSV-FJ04A毒株的组织病理学及病毒在体内分布研究

王隆柏,王晨燕,吴学敏,车勇良,陈如敬,周伦江   

  1. 福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所,福建省农业科学院畜牧兽医研究所
  • 收稿日期:2016-05-03 修回日期:2016-07-05 接受日期:2016-07-11 出版日期:2016-08-10 发布日期:2016-08-10
  • 通讯作者: 周伦江
  • 基金资助:
    省公益类科研院所专项“猪繁殖与呼吸综合征病毒多抗原表位基因串联表达与应用研究”(2014R1023-16);福建省科技创新平台建设项目“猪主要病毒性疾病化学发光免疫检测技术的研究与应用”(2014N2003-4)。

PRRSV-FJ04A Distribution and Histopathology in Infected Piglets

周伦江   

  • Received:2016-05-03 Revised:2016-07-05 Accepted:2016-07-11 Online:2016-08-10 Published:2016-08-10

摘要: 旨在研究猪繁殖与呼吸综合征病毒福建04A(PRRSV-FJ04A)株感染仔猪后的组织病理变化和病毒在仔猪体内各器官的分布情况。采用PRRSV-FJ04A株人工接种30日龄PRRSV抗体阴性仔猪,通过组织学观察与荧光定量RT-PCR技术,对呼吸道、消化道、泌尿道主要器官及免疫器官等组织进行了病理和病毒抗原定量分析。结果表明,PRRSV-FJ04A毒株接种猪群10天,肺脏中有大量肺泡细胞变性、坏死,巨噬细胞增生;淋巴结淋巴细胞坏死;脑神经元变性、坏死,脾小体有大量细胞碎片,肝脏有颗粒变性,扁桃体隐窝上皮脱落等;PRRS病毒在猪群体内各器官含量分布含量以最高为肺脏,其次为淋巴结、脑、扁桃体、血液等,而在肾脏中病毒含量最低。

关键词: 大麦, 大麦, 苗期, 渍涝, 农艺性状, 秸秆饲用品质

Abstract: The experiment was conducted to study the histopathological changes of piglets infected by PRRSV-FJ04A and its distribution in the infected piglets. PRRSV-FJ04A strain was used to infect the 30-day-old healthy piglets. The pathological changes and distribution of virus in the organs including airtube, digestive canal, urinary tract, immune organs were analyzed by histopathology observation and real-time RT-PCR assay. The results showed that the piglets were killed 10 days after infected,that there were a lot of metamorphic and necrotic alveolar cells, hyperplastic macrophages in the lung. It also caused necrotic lymphocyte of lymphaden and neuron degeneration even neorobiosis of brain. And a lot of cell debris in acinus lienalis, granular degeneration of liver and exuviation of cryptae tonsillares were found. There were a large amount of virus in the lung, followed by lymph gland, brain, amygdala and blood. And kidney was found to have the least virus.