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中国农学通报 ›› 2017, Vol. 33 ›› Issue (16): 53-58.doi: 10.11924/j.issn.1000-6850.casb16060019

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

苹果果实角质层提取及分析方法的优化

陈剑婷,吴坤胜,周磊磊,宋欢,李沆,曲桂芹   

  1. 中国农业大学食品科学与营养工程学院,中国农业大学食品科学与营养工程学院,中国农业大学食品科学与营养工程学院,中国农业大学食品科学与营养工程学院,中国农业大学食品科学与营养工程学院,中国农业大学食品科学与营养工程学院
  • 收稿日期:2016-06-03 修回日期:2017-04-09 接受日期:2016-07-29 出版日期:2017-06-08 发布日期:2017-06-08
  • 通讯作者: 曲桂芹
  • 基金资助:
    863 课题“基于代谢组学的物流农产品保质减损技术研发子课题”(2012AA101702-2)“苹果代谢组学及减损技术研究”。

Optimization of Extraction and Analysis Method of Apple Fruit Cuticle

陈剑婷,吴坤胜,周磊磊,宋欢,李沆 and 曲桂芹   

  • Received:2016-06-03 Revised:2017-04-09 Accepted:2016-07-29 Online:2017-06-08 Published:2017-06-08

摘要: 苹果货架期失水是影响其品质的重要因素,而果实角质层又是水分散失的主要屏障,探讨苹果货架期角质层和果实失水率的相关性尤为重要,而对角质层组分的定性和定量测定,展开相关研究的前提。本研究本研究针对现有对苹果果实角质层提取和分析的方法进行比较优化,为研究货架期苹果果实角质层的代谢变化及苹果果实采后综合减损措施的探求提供方法学基础。研究结果表明,利用氯仿溶液处理完整果实1 min、完整果实1 h、表皮圆片1 min、表皮圆片1 h,发现氯仿处理表皮圆片1 h 所得蜡质含量最高,蜡质成分种类及占比,与其他三者无显著差异,是苹果果实蜡质提取的最优方法。在苹果果实角质分析方面,证明了目前普遍应用于番茄果实角质分析的MeOH-HCl 试剂,对苹果果实角质的解聚具有稳定可靠的效果,苹果角质所得的单体产率可达92%左右。

关键词: 微生物菌剂, 微生物菌剂, 连栋大棚, 土壤养分, 硝态氮

Abstract: Apple fruit water loss is one of the important factors affecting its quality during shelf life. And the fruit cuticle is the main barrier of water loss. It is particularly important to explore the correlation between apple fruit cuticle and fruit water loss rate. And quantitative determination of the components of apple fruit cuticle is the premise of the research. This paper optimized the method of extraction and analysis of apple fruit cuticle, which provided a train of thought for searching postharvest preservation measures of apple fruit. The specific research results were as follows: treating the intact fruit for 1 min, intact fruit for 1 h, fruit discs for 1 min, fruit discs for 1 h by chloroform, the total wax content from treating fruit discs for 1 h was the highestamong them, and there was no significant difference between their wax component types and proportions. For apple fruit cutin analysis, MeOH- HCl reagent was used to depolymerize apple fruit cutin instead of the previously depolymerization method, verified it was effective for apple fruit. And the monomer production rate of apple fruit cuticle could reach 92%.

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