水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究

doi:10.11924/j.issn.1000-6850.casb18060030

中国农学通报 ›› 2019, Vol. 35 ›› Issue (14): 1-7.doi: 10.11924/j.issn.1000-6850.casb18060030

所属专题：生物技术；水稻；农业生态

• 农学农业基础科学 • 下一篇

水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究

张瑛¹, 李浩¹, 张雨萱², 滕斌¹, 杨剑波¹

1.安徽省农业科学院水稻研究所;2.华中师范大学生命科学学院

收稿日期:2018-06-06 修回日期:2018-08-15 接受日期:2018-08-27 出版日期:2019-05-15 发布日期:2019-05-15
通讯作者: 张瑛
基金资助:
国家重点研发计划课题项目“沿江平原稻稻和稻麦两熟区周年节本丰产增效关键技术研究与模式构建”(2017YFD0301304)；国家重点研发计划课题项目“适应高温逆境单季籼稻绿色轻简化栽培技术构建与环境效益评估”(2017YFD0300106)；安徽省科技攻关“适宜杂交水稻机械化制种的资源创制与品种培育”(1604a0702008)；安徽省农科院学科建设项目“影响水稻耐储藏关键基因的调控模式”(17A0105)。

Study on the Correlation Between Chlorate Resistance and Nitrate Reductase (NR), Nitrite Reductase (NIR) of Oryza sativa

Received:2018-06-06 Revised:2018-08-15 Accepted:2018-08-27 Online:2019-05-15 Published:2019-05-15

摘要/Abstract

摘要： 氯酸钾抗性是水稻籼粳分类重要指标之一,深入研究氯酸钾抗性机制对揭示籼粳分化的遗传生理基础具有理论和实践意义。本文利用4份籼稻材料和4份粳稻材料,研究硝酸还原酶NR、亚硝酸还原酶NIR与水稻氯酸钾抗性的关联性。结果表明：NR酶活与水稻氯酸钾反应指数呈显著正相关 (r=0.716*,显著性水平0.046),NIR酶活与水稻的氯酸钾反应指数相关性不显著(r=-0.628,显著性水平0.096),而NIR/NR比值与水稻的氯酸钾反应指数呈极显著负相关(r=-0.879**,显著性水平0.004)；当NR活性受抑制时,则NIR酶活与水稻氯酸钾反应指数呈显著负相关(r=-0.709*,显著性水平0.049)。由此推断：NIR/NR的相对大小影响了水稻氯酸钾抗性的强弱。这对深入揭示水稻氯酸钾抗性机理有重要参考价值。

关键词: 秸秆还田, 秸秆还田, 小麦-玉米轮作, 土壤养分库容量, 土壤微生物

Abstract: Chlorate resistance is one of the reliable characters in Indica/Japonica classification. To understand the mechanism of chlorate resistance is very important for revealing the evolutionary mechanism of Indica/ Japonica differentiation. In this study, 4 Indica materials and 4 Japonica materials were used to detect the correlation between nitrate reductase (NR), nitrite reductase (NIR) and chlorate resistance. NR activity showed a significantly positive correlation with chlorate response index (r=0.716*, 0.046 significance level), and the correlation of NIR activity and chlorate response index was not significant (r=- 0.628, 0.096 significance level), whereas the ratio of NIR/NR showed a very significantly negative correlation with chlorate response index (r=- 0.879**, 0.004 significance level). When NR activity was inhibited partially, NIR activity was changed with a significantly negative correlation with chlorate response index (r=-0.709*, 0.049 significance level). Therefore, we deduce that the ratio of NIR/NR may be a strong influence on chlorate resistance, and a great value for revealing the mechanism of chlorate resistance of Oryza sativa.

张瑛,李浩,张雨萱,滕斌,杨剑波. 水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究[J]. 中国农学通报, 2019, 35(14): 1-7.

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水稻氯酸钾抗性与硝酸还原酶NR、亚硝酸还原酶NIR的关联性研究

Study on the Correlation Between Chlorate Resistance and Nitrate Reductase (NR), Nitrite Reductase (NIR) of Oryza sativa

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