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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (29): 30-37.doi: 10.11924/j.issn.1000-6850.casb2024-0695

• 林学·园艺·园林 • 上一篇    下一篇

基于代谢组学的文山本地蘘荷种质资源评价

王灿1(), 徐杰1(), 赵大伟1, 石瑶2(), 孙宏伟1, 袁理春2, 周琼3, 张云波4, 周明敏5   

  1. 1 文山州农业科学院， 云南文山 663000
    2 云南省农业科学院药用植物研究所， 昆明 650205
    3 云南省曲靖市会泽县种子管理站， 云南曲靖 655000
    4 红河州农业科学院， 云南蒙自 661199
    5 文山学院三七医药学院， 云南文山 663000
  • 收稿日期:2024-11-14 修回日期:2025-02-15 出版日期:2025-10-15 发布日期:2025-10-22
  • 通讯作者:
    徐杰，男，1979年出生，文山广南人，研究员，学士，主要从事中药材新品种选育与栽培方面的研究。E-mail：；
    石瑶，女，1982年出生，云南昆明人，高级实验师，硕士，研究方向：药用植物资源与利用研究。E-mail：。
  • 作者简介:

    王灿，男，1993年出生，山西长治人，农艺师，硕士，研究方向：药用植物遗传育种。通信地址：663000 云南省文山州文山市泰康西路2号 文山州农业科学院，Tel：0873-3037546，E-mail：。

  • 基金资助:
    文山州袁理春专家工作站“蘘荷资源调查与栽培研究示范项目”(WS2021-5)

Assessment of Local Zingiber mioga Germplasm Resources in Wenshan Based on Metabolomic

WANG Can1(), XU Jie1(), ZHAO Dawei1, SHI Yao2(), SUN Hongwei1, YUAN Lichun2, ZHOU Qiong3, ZHANG Yunbo4, ZHOU Mingmin5   

  1. 1 Wenshan Prefecture Academy of Agricultural Sciences, Wenshan, Yunnan 663000
    2 Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650205
    3 Seed Management Station of Huize County, Qujing, Yunnan 655000
    4 Honghe Academy of Agricultural Sciences, Mengzi, Yunnan 661199
    5 College of Panax notoginseng, Wenshan University, Wenshan, Yunnan 663000
  • Received:2024-11-14 Revised:2025-02-15 Published:2025-10-15 Online:2025-10-22

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摘要：

蘘荷为食药兼用保健型植物资源，为探明文山地区不同蘘荷品种花苞的代谢产物差异，本研究以 2 个自主选育品种（‘文荷1号’‘文荷2号’）及1个当地自留种（CK）的蘘荷花苞为试验材料，采用高效液相色谱-串联质谱（HPLC-MS/MS）结合广泛靶向代谢组学技术，分析不同品种花苞的代谢产物组成及代谢通路差异。结果表明‘文荷2号’维生素C含量最高，粗纤维含量以自留种最高。蘘荷花苞中测定的代谢物共758个，氨基酸及其衍生物145个、类黄酮110个等。其中‘文荷1’号与‘文荷2号’总差异代谢物为94个，‘文荷1号’与对照总差异代谢物为65个，‘文荷2号’与对照总差异代谢物为87个，主要体现在氨基酸及其衍生物和糖类及其衍生物中。代谢通路的富集分析表明，与对照相比，‘文荷1号’和‘文荷2号’在氨基酸合成、芥子油苷合成等功能化合物合成途径中相关代谢物显著上调。此外在‘文荷1号’与‘文荷2号’比较中，‘文荷1号’显著上调的代谢途径有戊糖磷酸途径、光合生物的碳固定等，这些均与光合作用有关。相同条件下‘文荷1号’、‘文荷2号’与本地种花苞代谢物存在显著差异，尤其在氨基酸合成、芥子油苷合成等具有利用价值的代谢物方面，这为蘘荷品种的功能性选育以及有效代谢组研究利用提供了数据参考。

关键词: 蘘荷, 花苞, 文山地区, 代谢组差异, 代谢通路差异, 维生素C, 纤维素, 种质资源

Abstract:

Zingiber mioga (Thunb.) Roscoe is a plant resource with food and medicinal value, as well as health-promoting functions. This study aims to investigate the metabolic product differences in flower buds among various Zingiber mioga (Thunb.) Roscoe varieties in the Wenshan Region. In this study, flower buds from two independently selected varieties (‘Wenhe1’, ‘Wenhe2’) and one locally preserved variety of Zingiber mioga (Thunb.) Roscoe were used as experimental materials. An extensive targeted metabolomics analysis utilizing high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was conducted to investigate the differences in metabolites and metabolic pathways among the flower buds of Zingiber mioga (Thunb.) Roscoe varieties. The findings indicated that ‘Wenhe2’ had the highest vitamin C content, whereas the landrace variety possessed the highest crude fiber content. In total, 758 metabolites were identified in Zingiber mioga (Thunb.) Roscoe buds, including 145 amino acids and derivatives, and 110 flavonoids. Among these metabolites, 94 were differentially expressed between ‘Wenhe1’and ‘Wenhe 2’, 65 between ‘Wenhe1’ and the control group, and 87 between‘Wenhe2’ and the control group. These variations predominantly occurred in amino acids and their derivatives, as well as sugars and their derivatives. Metabolic pathway enrichment analysis revealed that both tested varieties exhibited significant upregulation in pathways related to amino acid synthesis and sinigrin synthesis compared to the control group. Additionally, in the comparison between ‘Wenhe1’ and ‘Wenhe2’, the metabolic pathways significantly upregulated in ‘Wenhe1’ included the pentose phosphate pathway and carbon fixation in photosynthetic organisms, which were strongly associated with photosynthesis. Under identical conditions, significant differences were observed in the metabolite profiles of ‘Wenhe1’ and ‘Wenhe2’ compared to local varieties, especially in metabolites of practical significance, including those involved in amino acid synthesis and glucosinolate synthesis. These results offer valuable insights for the functional breeding of Zingiber mioga (Thunb.) Roscoe and the exploration and utilization of effective metabolomic resources.

Key words: Zingiber mioga (Thunb.) Roscoe, flower bud, Wenshan, metabolomic differences, metabolic pathway differences, vitamin C, cellulose, germplasm resources