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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (10): 49-53.doi: 10.11924/j.issn.1000-6850.casb14100108

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

萝卜幼苗在盐胁迫下的生理响应研究

梅 燚,祖艳侠,吴永成,郑佳秋,王薇薇,郭 军   

  1. (江苏沿海地区农业科学研究所,江苏盐城 224002）
  • 收稿日期:2014-10-26 修回日期:2014-12-04 接受日期:2014-12-18 出版日期:2015-05-05 发布日期:2015-05-05
  • 通讯作者: 郭 军
  • 基金资助:
    江苏省科技支撑计划项目“优质、高产、抗病耐抽薹设施专用萝卜新品种选育”(BE2012342)；江苏省农业科技自主创新资金项目“优质、多抗、耐热萝卜新品种选育”[CX(13)2007]；江苏省盐土生物资源研究重点实验室开放课题“萝卜耐盐资源筛选与耐盐组合选育”(JKLBS2012018)；江苏沿海地区农业科学研究所科研基金“盐胁迫对萝卜生长的影响与缓解措施的研究”(YHS201106)。

The Physiological Responses of NaCl Stress on Radish Seedlings

Mei Yi, Zu Yanxia, Wu Yongcheng, Zheng Jiaqiu, Wang Weiwei, Guo Jun   

  1. (Institute of Agricultural Sciences in the Coastal Area Jiangsu, Yancheng Jiangsu 224002)
  • Received:2014-10-26 Revised:2014-12-04 Accepted:2014-12-18 Online:2015-05-05 Published:2015-05-05

摘要： 旨在研究不同强度的盐胁迫对萝卜幼苗的影响及其生理响应机制，为萝卜的抗盐机制研究和耐盐品种的选育做一些前期研究。在人工基质培养条件下，通过往营养液中加入NaCl，配置成不同浓度盐溶液(0、50、100、150、200 mmol/L)，以此模拟盐胁迫逆境。在幼苗生长方面，随盐分的升高，叶片鲜重、地上部鲜重等7个指标出现明显下降，在盐浓度超过100 mmol/L以后，这些指标都与对照有显著性差异；而地下部鲜重、地下部干重2个指标随盐分增加出现了先升高后下降的趋势。幼苗期的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性随盐分升高表现为先降低，后升高，最后再降低的规律。相对叶绿素含量呈现下降趋势，丙二醛(MDA)呈现升高趋势，可溶性蛋白含量呈现升高趋势，但在200 mmol/L浓度下，迅速下降。盐胁迫抑制萝卜幼苗的生长，并且逆境强度越强，抑制越明显。抗氧化酶活性、可溶性蛋白含量在盐胁迫下起到了维持细胞膜稳定的作用；相对叶绿素、丙二醛的含量能较好地反映出幼苗受盐害的程度。

关键词: 地形, 地形, 模型, 土壤, 可移植性

Abstract: The aim was to investigate the effects of salt stress on radish seedling and its physiological response mechanism, thus to make a preliminary study on salt-tolerance mechanism and screen out salt tolerant cultivars. The seedling growth was studied under artificial medium cultivation with 5 NaCl concentrations (0, 50, 100, 150, 200 mmol/L). With the increase of salt concentration, the seven indexes such as fresh weight of blade and wet weight of above-ground reduced significantly. When the concentration was higher than 100 mmol/L, there were significant differences between control group and treatment group. While with the increase of NaCl concentration, root fresh weight and dry weight increased first and then decreased. Under increased NaCl stress, POD, CAT and SOD in seedling decreased first, and then increased, and decreased finally. The relative chlorophyll content in seed revealed a trend of decrease, while the content of MDA increased. Soluble protein content increased first and then declined rapidly at 200 mmol/L salt concentration. To sum up, the salt stress inhibited radish seedling growth, and the effect of inhibition increased with higher salt stress. Antioxidant enzyme activities and soluble protein content had the function of maintaining the steady structure of cell velum under NaCl stress. Relative chlorophyll content and MDA could reflect the level of salt injury of radish seedling.