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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (34): 30-35.doi: 10.11924/j.issn.1000-6850.casb18060037

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

甜菜M14品系咖啡酰辅酶A-O-甲基转移酶提高植物抗逆性功能分析

王宇光,吕笑言,季美超,宫世龙,李海英   

  1. 黑龙江大学 生命科学学院,黑龙江大学 软件学院,黑龙江大学 生命科学学院,黑龙江大学 生命科学学院,黑龙江大学 生命科学学院
  • 收稿日期:2018-06-08 修回日期:2018-11-15 接受日期:2018-08-24 出版日期:2018-12-16 发布日期:2018-12-16
  • 通讯作者: 李海英
  • 基金资助:
    国家自然科学基金“甜菜M14 品系抗氧化酶系统响应盐胁迫应答过程的研究”(31471552)，“甜菜T510 品系BvBHLH93 转录因子功能及其 耐盐调控机制分析”(31701487)；黑龙江省高校基本科研业务费“甜菜耐盐及盐敏感品系盐胁迫响应机制研究”(KJCXYB201706)。

Stress Tolerance Improvement by BvM14-CCoAOMT Gene in Sugar Beet M14 Strain

  • Received:2018-06-08 Revised:2018-11-15 Accepted:2018-08-24 Online:2018-12-16 Published:2018-12-16

摘要： 为研究甜菜M14品系咖啡酰辅酶A-O-甲基转移酶基因(BvM14-CCoAOMT)的功能，本研究采用叶盘法将M14品系咖啡酰辅酶A-O-甲基转移酶基因转化烟草，获得T1代转基因植株，而后以野生型及转空载体植株为对照，分别在对照、100 mmol/L NaCl、200 mmol/L NaCl、100 mmol/L甘露醇及200 mmol/L甘露醇的培养条件下进行植株生理指标的抗逆性分析。研究发现在100 mmol/L NaCl、200 mmol/L NaCl盐胁迫条件下，转基因植株的鲜重、木质素含量以及叶绿素含量均显著高于对照植株。此外，研究结果表明转基因植株在100 mmol/L甘露醇胁迫条件下根长和叶绿素含量显著高于对照植株，如转基因植株的根长和叶绿素含量分别为0.68 cm和 0.31 mg，而野生型植株的根长和叶绿素含量分别为0.51 cm 和0.18 mg。研究结果表明转M14品系咖啡酰辅酶A-O-甲基转移酶基因烟草植株对盐和干旱胁迫具有较强抗性，这为进一步深入研究M14品系咖啡酰辅酶A-O-甲基转移酶基因功能奠定重要基础。

关键词: 连作障碍, 连作障碍, 土传病害, 自毒作用, 间作

Abstract: To study the function of caffeoyl CoA O-methyltransferase gene (BvM14-CCoAOMT) in sugar beet M14 strain, the transgenic plants of T1 generation were obtained by the method of leaf disc method, and the resistance of positive transgenic plants was analyzed. Furthermore, compared with wild type and tramsformed empty carrier plants, the resistance of positive transgenic plants were analyzed under the conditions of control, 100 mmol/L NaCl, 200 mmol/L NaCl, 100 mmol/L mannitol and 200 mmol/L mannitol. It was found that under 100 mmol/L NaCl and 200 mmol/L NaCl, the fresh weight, lignin content and chlorophyll content of the transgenic plants were significantly higher than those of control plants. In addition, the results showed that the root length and chlorophyll content of transgenic plants under 100 mmol/L mannitol were significantly higher than those of the control plants. For example, the root length and chlorophyll content of transgenic plants were 0.68 cm and 0.31 mg respectively, while the root length and chlorophyll content of wild type plants were 0.51 cm and 0.18 mg respectively. The results showed that transgenic tobacco plants with BvM14-CCoAOMT gene had strong resistance to salt and drought stress, and provide the theoretical basis for the further study of BvM14-CCoAOMT function.

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