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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (18): 197-202.doi: 10.11924/j.issn.1000-6850.casb2025-0824

• 植物保护·农药 • 上一篇    下一篇

云南主要烟产区真菌病原的分离与ITS序列分析

白子力1(), 陈泽斌2(), 徐胜光3, 潘小梅4, 范志伟1, 苏源1, 田智诚1, 杨嘉欣1, 向锦茜1   

  1. 1 昆明学院农学与生命科学学院， 昆明 650214
    2 昆明学院教务处， 昆明 650214
    3 云南省高校生物炭工程研究中心， 昆明 650214
    4 宜春学院生命科学与资源环境学院， 江西宜春 336000
  • 收稿日期:2025-09-30 修回日期:2026-01-11 出版日期:2026-09-25 发布日期:2026-09-24
  • 通讯作者:
    陈泽斌，男，1985年出生，云南昆明人，教授，博士，主要从事烟草连作障碍的成因及消减技术。通信地址：650214 云南省昆明市昆明经济技术开发区浦新路2号 昆明学院，教务处，Tel：18687102882，Email：。
  • 作者简介:

    白子力，男，2001年出生，云南新平人，硕士研究生，研究方向：烟草主要病害病原生理分化研究。通信地址：650214 云南省昆明市昆明经济技术开发区浦新路2号 昆明学院，农学与生命科学学院，Tel：18214176710，E-mail：。

  • 基金资助:
    云南省地方本科高校基础研究联合专项重点项目“苯甲酸降解菌SG12消减草莓连作障碍的作用机理研究”(202401BA070001-004); 云南省“兴滇英才支持计划”—青年人才专项

ITS Sequence Analysis and Isolation of Fungal Pathogens in Major Tobacco Producing Areas of Yunnan Province

BAI Zili1(), CHEN Zebin2(), XU Shengguang3, PAN Xiaomei4, FAN Zhiwei1, SU Yuan1, TIAN Zhicheng1, YANG Jiaxin1, XIANG Jinxi1   

  1. 1 College of Agronomy and Life Sciences, Kunming University, Kunming 650214
    2 Academic Affairs Office, Kunming University, Kunming 650214
    3 Yunnan Provincial University Biochar Engineering Research Center, Kunming 650214
    4 College of Life Sciences and Resources and Environmental Sciences, Yichun University, Yichun, Jiangxi 336000
  • Received:2025-09-30 Revised:2026-01-11 Published:2026-09-25 Online:2026-09-24

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摘要：

为明确云南主要烟区烟草病原真菌的种类组成，本研究采集了云南主要烟区新近发病的烟草植株及其组织为试材，通过ITS序列的PCR扩增与测序技术，测得39株真菌的鉴定数据。对这些序列与美国国家生物技术信息中心(NCBI)数据库中的序列进行同源性比对，应用MEGA软件构建系统发育树。结果表明，其中33株真菌能鉴定至种的水平，与12种已知真菌的ITS序列同源性较高；其余6株真菌仅能确定至属的水平。镰刀菌属真菌(Fusarium spp.)共分离到13株，占分离总数的33%，是该地区烟草病原真菌中的优势类群。总体而言，从云南植烟区病株中共分离鉴定出39株真菌，依据ITS序列分析，可划分为12个种、15个类群，其中镰刀菌属是主要的优势类群。

关键词: 云南, 烟草, 病原真菌, 分离, ITS序列, 鉴定, 镰刀菌属

Abstract:

To identify and analyze the species composition of pathogenic fungi isolated from diseased tobacco plants in the major tobacco-growing regions of Yunnan, newly infected tobacco plants and their tissues collected from these areas were used as test materials. Fungal identification was performed by ITS sequence PCR amplification and sequencing, yielding data for 39 fungal isolates. The obtained sequences were compared with those in the US National Center for Biotechnology Information (NCBI) database for homology analysis, and a phylogenetic tree was constructed using MEGA software. The results showed that 33 isolates could be identified to the species level, with high sequence homology to 12 known fungal species, whereas the remaining six isolates were identified only to the genus level. Among the isolates, 13 belonged to the genus Fusarium, accounting for 33% of the total. In total, 39 fungal isolates were recovered from diseased plants in Yunnan tobacco-growing areas; ITS analysis identified 12 species across 15 taxonomic groups, with Fusarium spp. representing the highest proportion and constituting the dominant group.

Key words: Yunnan, tobacco, pathogenic fungi, isolation, ITS sequence, identification, Fusarium

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