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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (3): 171-176.doi: 10.11924/j.issn.1000-6850.casb2025-0378

• 植物保护·农药 • 上一篇    下一篇

哈茨木霉与不同微生物菌剂混配对甜菜褐斑病病菌的联合毒力测定及田间防效

高博(), 郝永丽(), 李泽霖, 刁佳美, 刘庆鹏, 张玮   

  1. 赤峰市农牧科学院， 内蒙古赤峰 024000
  • 收稿日期:2025-05-21 修回日期:2025-10-11 出版日期:2026-02-15 发布日期:2026-02-09
  • 通讯作者:
    郝永丽，女，1981年出生，内蒙古赤峰人，研究员，硕士，主要从事经济作物栽培及病虫害防治的研究工作。通信地址：024000 内蒙古赤峰市松山区科研路农牧科技产业园 赤峰市农牧科学院，E-mail：。
  • 作者简介:

    高博，女，1986年出生，内蒙古赤峰人，副研究员，硕士，主要从事经济作物栽培及病虫害防治的研究工作。通信地址：024000 内蒙古赤峰市松山区科研路农牧科技产业园 赤峰市农牧科学院，E-mail：。

  • 基金资助:
    赤峰市农牧科学院院内资助项目“赤峰地区甜菜褐斑病致病性分析及其绿色高效防控模式研究与应用”(2023CFNKS02); 内蒙古自治区现代农牧业产业技术体系建设专项资助(IMARS-15)

Trichoderma harziensis Mixed with Different Microbial Agents for Cercospora Leaf Spot: Determination of Combined Virulence and Field Control Efficacy

GAO Bo(), HAO Yongli(), LI Zelin, DIAO Jiamei, LIU Qingpeng, ZHANG Wei   

  1. Chifeng Academy of Agricultural and Animal Husbandry Sciences, Chifeng, Inner Mongolia 024000
  • Received:2025-05-21 Revised:2025-10-11 Published:2026-02-15 Online:2026-02-09

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摘要：

针对内蒙古甜菜褐斑病危害严重，化学防治易产生耐药性，生物防治缺乏高效复配菌剂的问题，为研发甜菜褐斑病绿色防控技术，以甜菜尾孢菌为靶标，采用菌丝生长速率法测定5种微生物菌剂的单剂毒力，筛选优势菌剂与哈茨木霉进行不同比例复配，通过联合毒力测定（共毒系数法CTC）筛选最优配比，并开展田间防效试验。结果显示：（1）单剂毒力排序为哈茨木霉（EC50=0.10 μg/mL）>多粘类芽孢杆菌（EC50=0.90 μg/mL）>胶冻样芽孢杆菌>解淀粉芽孢杆菌>枯草芽孢杆菌；（2）哈茨木霉与4种菌剂复配均表现增效，其中5×1010 cfu/g多粘类芽孢杆菌与1×1010 cfu/g哈茨木霉6:4混配增效作用显著，EC50值为0.16 μg/mL，共毒系数为139.35；（3）该最优配比田间防效为60.94%，显著高于各单剂（38.62%~50.64%）。综上，5×1010 cfu/g多粘类芽孢杆菌与1×1010 cfu/g哈茨木霉6:4复配菌剂对甜菜褐斑病防效优异，可作为该病生物防控的优选方案。未来可深入探究复配菌剂的协同增效机制，优化制剂配方与施用技术，为甜菜褐斑病绿色防控提供更完善的技术支撑。

关键词: 甜菜褐斑病菌, 哈茨木霉, 微生物菌剂, 毒力测定, 田间防效

Abstract:

In response to the severe damage caused by Cercospora leaf spot (Cercospora beticola) on sugar beets in Inner Mongolia, and the issues of chemical control leading to resistance and a lack of highly effective compound microbial agents for biological control, this study aimed to develop green control technology for this disease. Using Cercospora beticola as the target pathogen, the individual toxicity of five microbial agents was determined via the mycelial growth rate method. Promising agents were selected and combined with Trichoderma harzianum in different ratios. The optimal combination ratio was screened through joint toxicity tests (Co-toxicity coefficient method, CTC), followed by field efficacy trials. The results showed that: (1) the order of individual toxicity was Trichoderma harzianum (EC50=0.10 μg/mL)> Paenibacillus polymyxa (EC50=0.90 μg/mL) > Bacillus mucilaginosus > Bacillus amyloliquefaciens > Bacillus subtilis. (2) Combinations of T. harzianum with the other four agents all showed synergistic effects. Among them, the mixture of 5×1010 cfu/g P. polymyxa and 1×1010 cfu/g T. harzianum at a 6:4 ratio exhibited significant synergy, with an EC50 value of 0.16 μg/mL and a co-toxicity coefficient of 139.35. (3) The field efficacy of this optimal combination reached 60.94%, significantly higher than that of individual agents (38.62%-50.64%). In conclusion, the compound agent of 5×1010 cfu/g P. polymyxa and 1×1010 cfu/g T. harzianum at a 6:4 ratio demonstrated excellent control efficacy against Cercospora leaf spot and can be recommended as a preferred option for its biological control. Future research should focus on elucidating the synergistic mechanisms of compound agents, optimizing formulation and application techniques to provide more robust technical support for the green control of Cercospora leaf spot in sugar beets.

Key words: Cercospora beticola, Trichoderma harzianum, microbial agent, virulence measurement, field control efficacy