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中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 242-248.

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

基于液-液萃取机理的新型环境样品前处理方法研究进展

杨春蕾 曹学丽   

  • 收稿日期:2010-12-27 修回日期:2011-01-09 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    复杂环境样品的高速逆流色谱前处理方法研究

Review of New Pretreatment Methods for Environmental Sample Based on Liquid-Liquid Extraction Mechanism

  • Received:2010-12-27 Revised:2011-01-09 Online:2011-03-20 Published:2011-03-20

摘要:

新型环境样品前处理技术已经逐步取代传统液-液萃取。液相微萃取、液膜萃取和逆流色谱技术均是集采样、萃取和浓缩于一体的环境友好型环境样品前处理技术。液相微萃取、液膜萃取实质上是微型化的液-液萃取;逆流色谱结合了液液萃取和分配色谱的优点,是一种新型色谱分离技术。3种技术均对环境样品中的痕量/超痕量污染物质有高效的富集、净化效果。笔者综述了液相微萃取、液膜萃取和逆流色谱技术的优缺点及在环境样品前处理中的应用。

关键词: 乙醛脱氢酶基因, 乙醛脱氢酶基因, 植物表达载体, 转化, 番茄

Abstract:

Traditional liquid-liquid extraction has been replaced by new environmental sample preparation techniques gradually. Liquid-phase microextraction (LPME), liquid membrane extraction (LME) and counter-current chromatography (CCC) are environment-friendly pretreatment techniques for environment sample which complete sampling, extracting and concentration in one step. LPME and LME are essentially miniaturized liquid-liquid extraction; CCC combines advantages of liquid-liquid extraction and partition chromatography as a new type of chromatographic separation techniques. All three techniques have high efficiency in trace/ultra-trace enriching and purification. The review introduced advantages and disadvantages of LPME, LME and CCC and their application in environment sample.