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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 152-155.

• 林学 园艺 园林 • 上一篇    下一篇

丹参酮ⅡA不同生育时期动态积累规律研究

王维婷 单成钢 房翠萍 陈庆亮 朱彦威 王志芬   

  • 收稿日期:2011-03-17 修回日期:2011-04-22 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    国家航天工程项目;农业部农业公益性行业科研专项经费项目“核技术农业应用”;山东省良种工程项目;山东省博士基金;山东省博士后创新项目专项

Study on the Dynamic Accumulation Regulation about Tanshinone ⅡA at Different Growth Stage

  • Received:2011-03-17 Revised:2011-04-22 Online:2011-09-05 Published:2011-09-05

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摘要：

为了解丹参酮ⅡA的积累规律，初步探索其合成机理。本研究选用7份丹参材料，测定了其不同生育时期丹参酮ⅡA含量及相对应的叶片中的叶绿素含量及过氧化物酶活性。结果表明：按照丹参酮ⅡA含量0.2%为标准，不同材料丹参酮ⅡA的积累规律略有不同；根中丹参酮ⅡA含量与相对应的叶片中的叶绿素a含量显著相关（rca＝0.458），与类胡萝卜素含量极显著相关（rxc=0.623），而与叶绿素b含量不相关（rcb＝0.069）；根中丹参酮ⅡA含量与相对应的叶片中POD酶活性的相关系数为0.577，呈现极显著相关性。花期丹参酮ⅡA含量有无下降现象与丹参酮ⅡA的合成效率有关；推测丹参酮ⅡA的合成途径的起始步骤可能与类胡萝卜素的合成更为类似，部分基因也与叶绿素a的合成相关基因相类似，与叶绿素b的起始步骤相差较远；丹参酮ⅡA可能与植物体内的生长素氧化有关，而POD可能也间接或直接参与了丹参酮ⅡA的合成调控。

关键词: 生产, 生产

Abstract:

To know the accumulate regulation about tanshinone ⅡA, preliminary study on its biosynthetic course. To known the accumulation regulation about tanshinone ⅡA, preliminary study had been done on the biosynthetic course. This study chosen 7 salvia strains as material, and their tanshinone ⅡA concentration in root, chlorophyll concentration in leaf and POD activity had been tested at different growth stage. Data shown that: the accumulation regulation about tanshinone could be divided by tanshinone ⅡA concentration at 0.2%; significant correlation existed between the tanshinone ⅡA concentration and chlorophyll a concentration in leaf (rca＝0.458), stronger significant correlation existed between the tanshinone ⅡA concentration and carotenoid concentration in leaf (rxc=0.623), while there was not correlation between the tanshinone ⅡA concentration and chlorophyll b concentration in leaf (rcb＝0.069). The coefficient of the tanshinone ⅡA concentration and POD activity was 0.577, significant correlation existed. Hence, it had been supposed that if the tanshinone ⅡA concentration’s decline or not at flowering stage related to tanshinone ⅡA biosynthetic efficiency; the start step about biosynthetic course of tanshinone ⅡA maybe looked like to the carotenoid, and some gene looked like to chlorophyll a, but disliked to chlorophyll b; while tanshinone ⅡA related to the oxidation of auxin, and POD may attached to the biosynthesis of tanshinone ⅡA directly or indirectly.