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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (7): 13-16.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

白皮松组织培养研究

张利 闫辉 高文强 赵红霞 樊金会   

  • 收稿日期:2011-10-09 修回日期:2011-12-23 出版日期:2012-03-05 发布日期:2012-03-05
  • 基金资助:

    白皮松抗盐碱性的遗传改造

Study on the Tissue Culture of Pinus bungeana

  • Received:2011-10-09 Revised:2011-12-23 Online:2012-03-05 Published:2012-03-05

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摘要：

为建立高效的再生体系，以白皮松成熟胚，子叶为外植体，经过初代培养和继代增殖培养，研究了不同植物生长调节剂种类及浓度、活性炭、GA3在组培各阶段的作用。结果表明：适合子叶诱导芽的培养基为MS+6-BA 3 mg/L+NAA 0.1 mg/L+TDZ 0.01 mg/L，适合胚诱导芽的培养基为MS+6-BA 2 mg/L+NAA 0.1 mg/L；适宜的继代培养基为MS+NAA 0.05 mg/L；以预处理胚（切除下胚轴）为外植体适宜生根培养基为1/2MS+IBA 0.3 mg/L；活性炭对芽增殖无显著作用，随活性炭浓度提高增殖系数有下降趋势；适量的活性炭和GA3可有效促进芽伸长。

关键词: 沼液, 沼液, 冬小麦, 土壤质量

Abstract:

The aim was to establish efficient regeneration system. Mature embryo and cotyledon of Pinus bungeana were used as explants, after primary culture and propagation culture, the role of different plant growth regulator type and concentration, activated carbon, GA3 in the various stages of tissue culture were studied. The results showed that the best initiated medium for cotyledon was MS+6-BA 3 mg/L+NAA 0.1 mg/L+TDZ 0.01 mg/L, the best initiated medium for mature embryo was MS+6-BA 2 mg/L+NAA 0.1 mg/L. The optimum shoot proliferation medium was MS+NAA 0.05 mg/L. Pre-embryo (removal of hypocotyl) as explants, the optimum shoots rooting medium was 1/2MS+IBA 0.3 mg/L. Activated carbon could not promote shoot proliferation, growth coefficient decreases with the increase of carbon concentration. Right amount of activated carbon and GA3 effectively promoted the growth of buds.