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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (36): 41-45.

所属专题： 生物技术； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

导入普通野生稻DNA的水稻变异种质的耐盐性鉴定

吕学莲 白海波 李树华 蔡正云 高晓原   

  • 收稿日期:2012-04-26 修回日期:2012-06-19 出版日期:2012-12-25 发布日期:2012-12-25
  • 基金资助:

    国家科技支撑计划项目

Salt Tolerance of Rice Variation Germplasm with Transformed Common Wild Rice (Oryza rufipogon) DNA

  • Received:2012-04-26 Revised:2012-06-19 Online:2012-12-25 Published:2012-12-25

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摘要：

旨在研究导入普通野生稻DNA的水稻后代的耐盐性，从中筛选出耐盐性较强的材料，为水稻耐盐育种提供种质资源。以普通野生稻DNA导入到宁夏栽培水稻‘宁粳23号’中获得的10份变异后代为材料，研究各材料在盐胁迫下的发芽特性和幼苗生长情况。结果表明在170 mmol/L NaCl处理下，材料D42、D5-54、D5-27、D-10、D102、DJ18和D68的发芽势和发芽率均较高，相对盐害率较低，在盐浓度为120 mmol/L时，材料D5-27、DJ18、D5-54和D-10的耐盐性为强，地上和地下鲜、干重的盐处理与非盐处理的比值较高，材料D5-27、DJ18、D5-54和D-10的可溶性糖积累量高于其受体。材料D42、D5-54、D5-27、D-10、D102、DJ18和D68的芽期耐盐性较强，材料D5-27、DJ18、D5-54和D-10属于苗期较耐盐的材料。

关键词: 进展, 进展

Abstract:

The research aimed to study salt tolerance of rice variation germplasm with transformed common wild rice DNA, and screen out the materials with high salt tolerance, so as to provide germplasm for salt-tolerant rice breeding. Ten rice variation germplasms of ‘Ningjing 23’ were used to study germination and seedling growth. The results showed that when treated with 170 mmol/L NaCl, the germination level and germination rate of materials D42, D5-54, D5-27, D-10, D102, DJ18 and D68 were high, and its relative salt harm rate were low. With the solution containing 120 mmol/L NaCl, the salt tolerance of D5-27, DJ18, D5-54 and D-10 were strong, and the ratio of salt treatment and non-salt treatment of ground and underground, fresh and dry weights were high. The increased soluble sugar content of materials D5-27, DJ18, D5-54 and D-10 were higher than its receptor. Materials D42, D5-54, D5-27, D-10, D102, DJ18 and D68 had high salt tolerance in germinating stage, and materials D5-27, DJ18, D5-54 and D-10 had high salt tolerance in seedling stage.

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