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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 3-3.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪等孢球虫PCR诊断方法的建立

刘光辉,宁长申,张龙现,程俊贞,菅复春   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Development of PCR for Diagnosing Isospora Suis

Liu Guanghui, Ning Changshen, Zhang Longxian,Cheng Junzhen, Jian Fuchun   

  • Online:2007-09-05 Published:2007-09-05

PDF (PC)

647

摘要： 参照GenBank收录的Isospora suis 18SrRNA基因全序列（U97523），利用引物分析软件Oligo 6.57和Primer Premier 5.0设计了一对引物（上游引物：5'-tcctgcgagtactcatatgc-3'；下游引物：5'-gttcagctacgcataccttg-3'），首次扩增出猪等孢球虫分离株的18SrRNA基因序列，结合GenBank上登录的相关原虫序列，用DNAstar4.0软件比较其同源性，经Clustalx1.81序列比对，Paup4.0、Treeview3.0、Phylip种系发育关系软件分析后，证实该分离株为猪等孢球虫，并且河南不同地区之间的猪等孢球虫没有明显遗传差异

关键词: 棉花, 棉花, 河北省, 品种区域试验, 评价

Abstract: One pair of primer(P1：5'-tcctgcgagtactcatatgc-3'；P2：5'-gttcagctacgcataccttg-3') was designed by using Oligo 6.57，Primer Premier 5.0 according to the sequence published by the GenBank. 18SrRNA gene of I.suis which isolated from different area in Henan Province was amplifed succesfully for the first time by the primer. The result was analysis by Paup and Phylip revealed that the homology of I.suis isolated from different area in Henan Province was 100%，and there was no variation consisted in Henan Isospora suis

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