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中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 40-46.

• 生物技术科学 • 上一篇    下一篇

AFLP技术及其在干果遗传育种研究中的应用

白瑞霞,彭建营,张媛,李莉   

  • 收稿日期:2008-09-11 修回日期:2008-10-28 出版日期:2009-05-20 发布日期:2009-05-20

AFLP Molecular Marker and Its Application in Dry Fruit

Bai Ruixia, Peng Jianying, Zhang Yuan, Li Li   

  • Received:2008-09-11 Revised:2008-10-28 Online:2009-05-20 Published:2009-05-20

摘要: AFLP(扩增片段长度多态性)是检测DNA多态性的一种高效的分子标记技术,该技术的运用不需要预知基因组的序列特征,可适用于任何来源和各种复杂度的DNA,兼具RFLP技术的可靠性和RAPD技术的方便性。近年来,AFLP技术已在干果遗传育种研究中得到广泛应用。本文对AFLP技术的基本原理和反应程序进行了介绍,综述了其在干果种质资源研究、遗传图谱构建、基因标记、分子辅助选择、基因表达等方面的应用,并对AFLP技术存在的问题及其在干果遗传育种研究上的应用前景进行了探讨。

关键词: 生长抑素, 生长抑素, 基因疫苗, 免疫, 机制

Abstract: AFLP (Amplified fragment length polymorphism) is a highly efficient technique for DNA fingerprinting. The technique is applicable to any organism without prior sequence characterization of the target genome. It’s characterized with reliability of RFLP, as well as with simplify of RAPD. In recent years, AFLP markers have been widely applied to dry fruit genetics and breeding. In this paper, the basic principles and procedures were introduced. The application of AFLP in dry fruit germplasm resource research, genetic linkage map construction, gene location, molecular-assisted selection and gene expression were reviewed. Problems and prospects of AFLP application in dry fruit genetics and breeding were also briefly discussed.

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