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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 50-53.

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

两用核不育系水稻叶绿体DNA的比较研究

欧立军1,2,3,李金龙3,童梁3,梁云浩3,袁雅威3   

  • 收稿日期:2010-01-07 修回日期:2010-02-19 出版日期:2010-06-05 发布日期:2010-06-05
  • 基金资助:

    湖南省高校创新平台开放基金项目

Chloroplast DNA polymorphism of dual purpose genic male sterile lines

Ou Lijun1,2,3, Li Jinlong3, Tong Liang3, Liang Yunhao3, Yuan Yawei3   

  • Received:2010-01-07 Revised:2010-02-19 Online:2010-06-05 Published:2010-06-05

PDF (PC)

1008

摘要：

以典型籼稻和粳稻作对照，对湖南应用较广的几个两用核不育系进行叶绿体DNA的ORF100、ORF29-TrnCGCA片段长度以及rps16基因内含子和TrnTUGU-TrnLUAA转录间区2个片段序列进行比较研究。结果表明，株1S、陆18S、株77S、H628S、88S、白天鹭S、G0543S、G03S、1103S和W6154S的叶绿体DNA的ORF100、ORF29-TrnCGCA两片段的大小均与典型的籼稻一致，其他材料则与典型粳稻相同；叶绿体DNA碱基多态性较丰富的2个区域rps16基因内含子和TrnT UGU -TrnL UAA间区的测序后发现4个籼粳稻特异位点，这4个位点对两用不育系叶绿体籼粳属性的判断与ORF100、ORF29-TrnCGCA片段的结果一致。根据育出组合的数量和质量，带有粳型血缘细胞质的两用核不育系如准S、810S、、中心S、C185S和培矮64S等显现了一定的优势。研究结果说明，通过籼粳稻杂交选育具粳稻血缘的籼型不育系是提高杂交水稻产量的有效途径之一。

关键词: 尖孢镰刀菌, 尖孢镰刀菌, 瓜类, 致病性, β-D-葡萄糖苷酶

Abstract:

The lengths of open reading frame (ORF)100 and ORF29-TrnCGCA, the intronic sequence of rps16 and the transcribed spacer of TrnTUGU-TrnLUAA in chloroplast of dual purpose genic male sterile line rice are studied using indica types and japonica types as controls. The results show that the lengths of ORF100 and ORF29-TrnCGCA in Zhu 1S, Lu 18S, Zhu 77S, H628S, 88S, Baitianlu S, G0543S, G03S, 1103S and W6154S are similar to those of typical indica, the others are similar to those of typical japonica. There are four special indica/japonica markers and the type of all dual purpose genic male sterile line rice based these special indica/japonica markers are same with the differentiation by ORF100 and ORF29-TrnCGCA. The materials such Zhun S, 810S, Zhongxin S, C185S and Peiai 64S those have japonica-type blood have more superiority than others based their quantity and quality of combination. Therefore, using dual purpose genic male sterile lines with japonica -type blood is an effective way to improve production of hybrid rice.