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中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 409-412.

所属专题: 生物技术

• 水产 渔业 • 上一篇    下一篇

葛氏鲈塘鳢(Perccottus glenii)的染色体组型研究

陈辉 赵文阁   

  • 收稿日期:2010-01-19 修回日期:2010-03-01 出版日期:2010-07-05 发布日期:2010-07-05
  • 基金资助:

    黑龙江省教育科学“十一五”规划课题;哈尔滨师范大学大学生创新基金项目;哈尔滨师范大学蔡火石教育发展基金项目

Study On the Chromosome Karyotype of Perccottus glenii

  • Received:2010-01-19 Revised:2010-03-01 Online:2010-07-05 Published:2010-07-05

摘要:

以葛氏鲈塘鳢(Perccottus glenii)的鳃、肾及肠细胞为材料,用空气干燥法制片,用Leica显微镜进行观察,依据Levan等(1964)的分类标准进行其染色体的数目及组型分析。结果表明葛氏鲈塘鳢的染色体数目为2n等于44条,n=22。染色体总臂数(NF)为82。其中,亚中部着丝点染色体为18对,亚端部着丝点染色体为1对,端部着丝点染色体为3对。核型公式为2n=36sm+2st+6t。根据染色体形态分析的结果,没有发现性染色体的存在。

关键词: 植物, 植物, 多聚半乳糖醛酸酶抑制蛋白, 分子特性, 表达, 调控

Abstract:

The Karyotype of Perccottus glenii was studied through the chromosome metaphase prepared from the cells of the gill、kidney and intestines by the colchicine-hypotonic-air drying technique and Giemsa staining.It was observed by Leica microscope.The analysis of chromosome Karyotype of Perccottus glenii was according to Levan’s standard.The result shows that the number of the Chromosome of Perccottus glenii is 2n=44,n=22. Its fundamental arm number(NF) is 82. Its karyotype comprises 18 pairs of submetacentric chromsome, 1 pair of subtelocentric chromosome and 3 pair of telocentric chromosome.That means 2n=36sm+2st+6t.According to the analysis of the conformation of the chromosome ,there is no sex chromosome existing.