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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 1-5.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 •    下一篇

河南省非洲猪瘟感染情况筛查

杨霞 陈陆 孙彦婷 刘春生 常洪涛 郑鹿平 刘红英 王川庆   

  • 收稿日期:2010-04-06 修回日期:2010-05-21 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    国家支撑计划食品安全专项

Detection Of ASF Infection Condition In Henan

  • Received:2010-04-06 Revised:2010-05-21 Online:2010-11-05 Published:2010-11-05

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1042

摘要：

目的：对河南省猪瘟（CSF）流行区的猪群进行非洲猪瘟（ASF）感染的排查；方法：利用猪瘟抗原捕获ELISA试剂盒对27个猪场的猪瘟疑似病料进行CSFV鉴定，并用区别猪瘟强弱毒的RT-PCR检测方法鉴定猪瘟疑似病料。与此同时针对ASFV VP73蛋白基因设计一对引物利用PCR方法对ASFV进行排查；结果：河南省CSFV流行区没有ASFV的感染；且RT-PCR对CSFV的检出率高于ELISA ,两者差异显著。结果表明,尽管目前中国作为俄罗斯的邻国存在ASFV的威胁，但中国河南仍没有ASFV感染。

关键词: 西洋参, 西洋参, 连作, 酶活性, 根系活力

Abstract:

Detection of ASF infection in CSF epidemiological area of HeNan province; Methods: Suspected- CSF histologic materials of 27 pig farms in HeNan were detected and identified by the commercial antigen-capture ELISA assay and RT-PCR for Detection and Differentiation of Wild-type and Vaccine Viruses of Classical Swine Fever Virus (CSFV) . At the same time, Primers were designed according to the nucleotide sequence of VP73 gene of African swine fever virus(ASFV) and PCR assays were performed to Screen ASF infection; Result: None ASF infection were found in CSF epidemiological area of HeNan province; The detection rate of CSFV by RT- PCR was higher than that by the commercial antigen-capture ELISA assay, the difference was significant. Results showed that none ASF infection was found in HeNan province, though ASFV threat was existed in China as a neighboring country of Russia now.