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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (22): 264-270.

所属专题： 资源与环境； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

红籽瓜种质资源亲缘关系RAPD分析

柳唐镜 于海彬 张 棵 吴素萍   

  • 收稿日期:2010-07-30 修回日期:2010-08-29 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:

    广西农科院科技发展基金

Genetic Relationships Analysis of Red-seed Edible Seed Watermelon Germplasm Resources by Using RAPD Markers

  • Received:2010-07-30 Revised:2010-08-29 Online:2010-11-20 Published:2010-11-20

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摘要：

本研究利用RAPD标记对红籽瓜种质资源进行亲缘关系分析。12个引物在51份红籽瓜种质中共扩增出80条谱带，其中多态性谱带74条，多态性谱带比率为92.50%。红籽瓜种质间遗传相似系数为0.41~0.99，说明RAPD标记能够揭示红籽瓜材料间较高的遗传多样性。UPGMA聚类结果表明，在相似系数为0.812处，51份红籽瓜种质可分为8个组群。第Ⅰ、Ⅱ、Ⅲ组为野生西瓜，第Ⅳ、Ⅶ组为菜用西瓜，第Ⅴ、Ⅷ组为籽用西瓜，第Ⅵ组为栽培种与野生种杂交的中间类型籽瓜。基于RAPD标记数据主成分分析结果显示，前3个主成分累积方差贡献率为44.54%，其反映红籽瓜种质间的亲缘信息与聚类分析结果基本一致。红籽瓜种质资源的亲缘关系与地理来源关系不大。

关键词: 北沙参, 北沙参, SRAP, 分子标记, 体系优化

Abstract:

In this study, RAPD markers were used to analyze genetic relationships of 51 red-seed edible seed watermelon accessions. Twelve primers with excellent reproducibility and 80 bands were obtained totally, 74 of these were polymorphism bands, and the polymorphism rate was 92.50%. The genetic similarity coefficients were between 0.41 and 0.99, which indicated that RAPD markers could reveal the higher genetic diversity of these tested materials. The systematical cluster analysis conducted with UPGMA showed that 51 accessions were grouped into 8 clusters at coefficients 0.812. Principal component analysis showed that 3 principal constituents contribute 44.54% of cumulative variance, in accord with systematical cluster analysis. This study revealed that there was not significant relationship between these germplasm and source place.

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